大麦籽粒相关性状全基因组关联分析

doi:10.3969/j.issn.1004-1524.20230754

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (5): 997-1002.DOI: 10.3969/j.issn.1004-1524.20230754

大麦籽粒相关性状全基因组关联分析

栾海业¹(), 朱琳洁¹, 李钰¹, 孟炜¹, 刘雨倩¹, 徐肖², 刘方方¹, 沈会权²^,^*()

1.盐城师范学院,江苏盐城 224002
2.江苏沿海地区农业科学研究所,江苏盐城 224002

收稿日期:2023-06-09 出版日期:2024-05-25 发布日期:2024-05-29
作者简介:栾海业(1984—),男,山东潍坊人,博士研究生,主要从事麦类作物育种研究。E-mail: luanhaiye@163.com
通讯作者: * 沈会权,E-mail: 244693584@qq.com
基金资助:
国家大麦青稞产业技术体系(CARS-05);江苏省自然科学基金项目(BK20201215);盐城市农业重点研发计划(YCBN202308)

Genome-wide association analysis of barley grain related traits

LUAN Haiye¹(), ZHU Linjie¹, LI Yu¹, MENG Wei¹, LIU Yuqian¹, XU Xiao², LIU Fangfang¹, SHEN Huiquan²^,^*()

1. Yancheng Teachers University, Yancheng 224002, Jiangsu, China
2. Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, Jiangsu, China

Received:2023-06-09 Online:2024-05-25 Published:2024-05-29

摘要/Abstract

摘要：

大麦籽粒形状和千粒重是决定大麦产量及用途的主要因素,挖掘相关候选基因对于提高大麦产量和品质具有重要意义。以国内外具有代表性的242份大麦自然群体为研究对象,对粒长、粒宽和千粒重进行全基因组关联分析。研究结果表明,大麦籽粒大小和千粒重表型变异丰富,变异系数范围为6.65%~19.98%。粒长、粒宽与千粒重均呈极显著正相关,且千粒重与粒宽的相关性高于粒长的。3个性状共检测到52个相关的SNP位点,在7条染色体均有分布。通过比对分析,筛选了6个相关性状的候选基因,在7号染色体上的乙烯响应因子与粒宽、千粒重均相关,表明该基因存在一因多效的功能。研究结果为大麦产量、品质的遗传改良提供了基因资源和理论基础。

关键词: 大麦, 籽粒相关性状, 全基因组关联分析, 候选基因

Abstract:

Grain size and thousand grain weight is the main factors that determine the barley yield and uses. It is of great importance to mine candidate genes related to grain for improving the yield and quality of barley. In this study, genome-wide association study (GWAS) was carried out to detect the candidate genes associated with grain traits in natural population containing 242 barley germplasm resources. The results showed that these traits showed rich phenotypic variation, with the coefficient of variation ranged from 6.65%-19.98%. Thousand grain weight was significantly correlated with grain length and grain width. The correlation coefficient between thousand grain weight and grain width was appreciably higher than thousand grain weight and grain length. Fifty-two SNP locus were detected by GWAS on 7 chromosomes. BLAST analysis showed that six candidate genes related to grain traits were selected. The ethylene response factor on chromosome 7 was simultaneously correlated with grain width and thousand grain weight, indicating that there was a multi-effect function of this gene. The results of this study provide new genetic resources and theoretical basis for the genetic improvement of barley yield and quality.

Key words: barley, grain trait, genome-wide association analysis, candidate gene

中图分类号:

S512.3

栾海业, 朱琳洁, 李钰, 孟炜, 刘雨倩, 徐肖, 刘方方, 沈会权. 大麦籽粒相关性状全基因组关联分析[J]. 浙江农业学报, 2024, 36(5): 997-1002.

LUAN Haiye, ZHU Linjie, LI Yu, MENG Wei, LIU Yuqian, XU Xiao, LIU Fangfang, SHEN Huiquan. Genome-wide association analysis of barley grain related traits[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 997-1002.

图/表 6

参考文献 20

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性状 Trait	最小值 Min	最大值 Max	平均值 Mean±SD	变异系数 CV/%
粒宽Grain width/mm	2.80	4.10	3.55±0.29	8.01
粒长Grain length/mm	6.75	10.80	9.15±0.0.61	6.65
千粒重Thousand grain weight/g	21	65	46.4±9.4	19.98

性状 Trait	最小值 Min	最大值 Max	平均值 Mean±SD	变异系数 CV/%
粒宽Grain width/mm	2.80	4.10	3.55±0.29	8.01
粒长Grain length/mm	6.75	10.80	9.15±0.0.61	6.65
千粒重Thousand grain weight/g	21	65	46.4±9.4	19.98

性状 Trait	粒宽 Grain width	粒长 Grain length
粒宽Grain width
粒长Grain length	-0.019	1
千粒重	0.749^**	0.364^**
Thousand grain weight

性状 Trait	粒宽 Grain width	粒长 Grain length
粒宽Grain width
粒长Grain length	-0.019	1
千粒重	0.749^**	0.364^**
Thousand grain weight

性状 Traits	染色体 Chromosome	物理距离 Position/Mb	等位基因 Allele	P值 P value
粒宽Grain width	chr4H	6.36	G/A	2.07×10^-5
	chr7H	525.64	C/T	1.29×10^-5
粒长Grain length	chr1H	29.88	T/C	5.60×10^-7
	chr3H	48.33	A/C	6.14×10^-5
	chr5H	541.96	A/G	2.76×10^-11
千粒重Thousand grain weight	chr1H	358.25	G/A	1.06×10^-6
	chr7H	525.64	C/T	3.93×10^-5

大麦籽粒相关性状全基因组关联分析

Genome-wide association analysis of barley grain related traits

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性状 Traits	基因名称 Gene name	物理距离 Physical position/bp	基因注释 Gene annotation
粒宽 Grain width	HORVU.MOREX.r3.4HG0333750	6 367 244—6 367 823	异三聚体G蛋白β亚基 β-subunit (Gβ) of heterotrimeric G protein
	HORVU.MOREX.r3.7HG0719680	525 633 815—525 635 110	乙烯响应转录因子Ethylene-responsive transcription factor
粒长 Grain length	HORVU.MOREX.r3.1HG0012250	29 883 924—29 888 635	AP2-乙烯响应转录因子 AP2-like ethylene-responsive transcription factor
	HORVU.MOREX.r 3.3HG0236930	48 332 444—48 334 763	细胞分裂素氧化酶/脱氢酶 Cytokinin oxidase/dehydrogenase
	HORVU.MOREX.r3.5HG0516880	541 960 847—541 962 427	锌指蛋白Zinc finger protein, putative
千粒重	HORVU.MOREX.r3.1HG0053590	358 248 159—358 253 865	赖氨酸特异性脱甲基酶Lysine-specific demethylase
Thousand grain weight	HORVU.MOREX.r3.7HG0719680	525 633 815—525 635 110	乙烯响应转录因子 Ethylene-responsive transcription factor

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