小麦SWEET和SUT家族基因鉴定与表达分析

doi:10.3969/j.issn.1004-1524.20240599

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (9): 1825-1839.DOI: 10.3969/j.issn.1004-1524.20240599

小麦SWEET和SUT家族基因鉴定与表达分析

张均¹(), 张博¹, 胡碧博¹, 刘京亮¹, 张晓宇¹, 李春阳¹, 熊盛婷¹, 郭彬彬¹, 王秀存², 马超¹^,^*()

¹ 河南科技大学农学院, 河南洛阳 471000
² 洛阳市农业技术推广服务中心, 河南洛阳 471099

收稿日期:2024-07-09 出版日期:2025-09-25 发布日期:2025-10-15
作者简介:*马超,E-mail:machao840508@163.com
张均(1982—),女,河南洛阳人,博士,副教授,主要从事作物生理与分子生物学研究。E-mail:zhangjun0105@126.com
通讯作者: 马超
基金资助:
国家自然科学基金(32372227);河南省高等学校青年骨干教师培养计划(2021GGJS050);河南省科技研发计划联合基金项目(232103810021);河南科技大学大学生创新创业训练计划项目(2024425);河南科技大学大学生创新创业训练计划项目(2024427)

Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.)

ZHANG Jun¹(), ZHANG Bo¹, HU Bibo¹, LIU Jingliang¹, ZHANG Xiaoyu¹, LI Chunyang¹, XIONG Shengting¹, GUO Binbin¹, WANG Xiucun², MA Chao¹^,^*()

¹ College of Agriculture, Henan University of Science and Technology, Luoyang 471000, Henan, China
² Luoyang Agricultural Technology Extension Service Center, Luoyang 471099, Henan, China

Received:2024-07-09 Online:2025-09-25 Published:2025-10-15
Contact: MA Chao

摘要/Abstract

摘要：

糖外排转运蛋白(sugars will eventually be exported transporter, SWEET)和蔗糖转运蛋白(sucrose transporter, SUT)是植物的2种糖转运蛋白。为分析TaSWEET和TaSUT基因在小麦基因组中的进化特征、功能及其对外源糖的响应,本研究对小麦TaSWEET和TaSUT家族成员进行了全基因组鉴定,并对其系统发育关系、基因结构、启动子顺式作用元件和表达特性进行了分析。结果表明,小麦中包含101个TaSWEET基因和16个TaSUT基因,二者均分为4个亚家族。基因结构显示,TaSWEET和TaSUT家族成员的外显子和内含子数量存在差异,数量为4~18个。保守基序分析显示,TaSWEET家族和TaSUT家族成员中存在20个基序,各亚家族内的基序结构相似。染色体分布模式和同源性分析表明,TaSWEET家族和TaSUT家族成员在3个亚基因组上数量分布均匀,全基因组片段重复和串联重复促进了小麦TaSWEET扩增。亚细胞定位预测显示,多数TaSWEET家族成员和全部TaSUT家族成员定位于质膜上,TaSWEET家族成员含4~7个跨膜螺旋结构,TaSUT家族成员含8~12个跨膜螺旋结构。启动子元件分析表明,TaSWEET和TaSUT家族成员的启动子区富含大量生长发育类、激素响应类和非生物胁迫类顺式作用元件。小麦基因表达模式分析显示,大部分TaSWEET和TaSUT家族成员在小麦叶片中表达量较低,在叶、芽、根、穗和籽粒中分别有28、25、33、39、21个成员高表达。通过qRT-PCR检测发现,经外源糖处理后,TaSWEET和TaSUT家族基因在根中表达量全部下调,在叶中有3个基因在果糖处理下表达量上调,1个基因在3种外源糖处理表达量均上调。上述结果为深入研究TaSWEET和TaSUT基因在调控小麦生长发育与在外源糖处理下的响应提供了理论依据。

关键词: 小麦, TaSWEET家族, TaSUT家族, 糖转运蛋白, 基因表达

Abstract:

SWEETs(sugars will eventually be exported transporter) and SUTs(sucrose transporters) are two sugar transporters in plants that play important roles in carbohydrate transport. In order to analyze the evolutionary characteristics, functions and responses of TaSWEET and TaSUT genes in the wheat genome and their response to exogenous sugars, this study conducted genome-wide identification of wheat TaSWEET and TaSUT, and analyzed their phylogenetic relationships, gene structure, promoter sequence elements, and expression characteristics. 101 TaSWEET genes and 16 TaSUT genes had been identified in wheat genome, both of which could be divided into four subfamilies. The genetic structure exhibited differences in the number of exons and introns among the TaSWEET family and TaSUT family members,ranging from 4 to 18. Conservative motif analysis displayed that the TaSWEET family and TaSUT family members had 20 motifs, the motif structures within each subfamily were similar. Chromosome distribution pattern and homology analysis indicated that TaSWEET family and TaSUT family members were evenly distributed in the three subgenomes, and the segmental and tandem repeats of the whole genome promoted the amplification of TaSWEET genes. Subcellular localization prediction proved that most of the TaSWEET and all TaSUT members were located in the plasma membrane. TaSWEET contained 4-7 transmembrane helical structures, while TaSUT contained 8-12. The analysis of promoter elements forecasted that the TaSWEET and TaSUT families were rich in growth and development, hormone response and abiotic stress cis-acting elements. Wheat gene expression pattern analysis demonstrated that most of the TaSWEET and TaSUT family members expressed at low levels in wheat leaves, while 28, 25, 33, 39 and 21 members expressed at high levels in leaf, shoot, root, spike and grain, respectively. Through qRT-PCR detection, it was found that the expression levels of TaSWEET and TaSUT family genes were all down-regulated in roots after exogenous sugar treatment, while 3 genes were up-regulated in leaves under fructose treatment, and one gene was up-regulated under all three suger treatments. The above results provide a theoretical basis for further studying the regulation of growth and development of TaSWEET and TaSUT genes in wheat and their response to exogenous sugar treatment.

Key words: wheat, TaSWEET family, TaSUT family, sugar transporter, gene expression

中图分类号:

S512

张均, 张博, 胡碧博, 刘京亮, 张晓宇, 李春阳, 熊盛婷, 郭彬彬, 王秀存, 马超. 小麦SWEET和SUT家族基因鉴定与表达分析[J]. 浙江农业学报, 2025, 37(9): 1825-1839.

ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.)[J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.

图/表 11

表1 部分TaSWEET和TaSUT家族基因qRT-PCR所用的引物

Table 1 Primers used for qRT-PCR analysis of partial TaSWEET family and TaSUT family genes

登录号 Accession number	基因名称 Gene name	正向引物序列 Forward primer sequence(5'→3')	反向引物序列 Reverse primer sequence(5'→3')
TraesCS4A02G016400.1	TaSUT6	GTGGTGTGGGTGTCAAGCA	GGGCGAGGGAGACGATCT
TraesCSU02G136300.1	TaSUT16	GTCGCCGCTTCCCTCA	GACTTGCAGCCATCGCATAT
TraesCS3D02G403300.1	TaSWEET30	AAGACCAAGAGCGTGGAGTACA	GGTAGATGGCGTAGAGGATGA
TraesCS4A02G140500.1	TaSWEET32	TCTCTGCTCCGCTCTCACC	GTAGACGCTCCAGATGCCG
TraesCS6A02G218800.1	TaSWEET50	ACCGTCATCGGCGTCATAG	GTACTGCTCCACCGTCTTCTTT
TraesCS6B02G421800.1	TaSWEET62	CAAGACCAAGAGTGTGGAGTACA	CTCCTTGACCTCGCTTGC
TraesCS7A02G147300.1	TaSWEET72	AGCTGCCTGCTGTGGATG	GTGACGAGGGCGATGAGG
TraesCS1B02G283900.1	Actin	GTTCCAATCTATGAGGGATACACGC	GAACCTCCACTGAGAACAACATTACC

图1 小麦TaSWEET和TaSUT家族成员的系统发育关系

Fig.1 Phylogenetic relationships among members of TaSWEET family and TaSUT family in wheat

图2 小麦TaSWEET和TaSUT家族的基因结构与蛋白质保守基序 A,TaSWEET和TaSUT家族基因的系统进化树;B,TaSWEET和TaSUT家族蛋白的保守基序;C,TaSWEET和TaSUT家族基因的结构。CDS,编码序列;UTR,非翻译区。

Fig.2 Gene structure and conserved protein motifs in the TaSWEET family and TaSUT family of wheat A, Phylogenetic tree of the TaSWEET and TaSUT family genes; B, Conserved motifs of TaSWEET and TaSUT family proteins; C, Gene structure of TaSWEET and TaSUT family. CDS, Coding sequence; UTR, Untranslated region.

图3 小麦TaSWEET和TaSUT家族成员的保守基序

Fig.3 Conserved motifs of TaSWEET family and TaSUT family members in wheat

图4 小麦TaSWEET和TaSUT家庭基因的染色体分布红色曲线代表基因之间的串联复制。染色体条带颜色深浅表示基因密度的高低。Un指染色体位置未知。

Fig.4 Chromosomal distribution of TaSWEET family and TaSUT family genes in wheat The red curve represents tandem duplication between genes. The color of the chromosome bands indicates the density of the genes. Un indicates that the chromosomal position is unknown.

图5 小麦TaSWEET和TaSUT家族基因的区域共线性关系红线连接TaSWEET和TaSUT基因中具有同源性的染色体区域。环1和环2的条纹和线表示碱基缺失的位置。环3为基因密度热图。

Fig.5 Regional colinearity relationships of TaSWEET family and TaSUT family genes in wheat The red line connects the chromosomal regions of the TaSWEET and TaSUT genes with homology. The bar and lines of rings 1 and 2 indicate the location of base deletions. Ring 3 is the gene density heatmap.

表2 小麦TaSWEET和TaSUT基因的Ka/Ks分析结果(Ka/Ks值>1)

Table 2 Ka/Ks analysis result of TaSWEET family and TaSUT family genes in wheat (Ka/Ks value>1)

序列1 Sequence 1	序列2 Sequence 2	非同义替换率 Ka	同义替换率 Ks	非同义替换/同义替换 Ka/Ks	平均S位点 Average S-sites	平均N位点 Average N-sites
TaSWEET46	TaSWEET85	0.333 0	0.287 9	1.156 6	217.500 0	628.500 0
TaSWEET46	TaSWEET91	0.333 1	0.287 6	1.158 1	217.666 7	628.333 3
TaSWEET39	TaSWEET85	0.335 2	0.288 8	1.160 8	217.666 7	628.333 3
TaSWEET39	TaSWEET91	0.335 7	0.287 4	1.168 2	217.833 3	628.166 7
TaSWEET42	TaSWEET91	0.347 2	0.283 5	1.224 9	218.166 7	627.833 3
TaSWEET42	TaSWEET85	0.345 8	0.280 4	1.233 4	218.000 0	628.000 0

图6 小麦TaSWEET和TaSUT家族基因启动子的顺式作用元件不同的顺式作用元件用不同颜色的圆点表示。

Fig.6 Cis-acting elements in the promoters of wheat TaSWEET family and TaSUT family genes Different cis-acting elements are represented by the dots of different colors.

图7 小麦TaSWEET和TaSUT家族基因在不同器官的表达水平图例颜色从下到上表示基因表达量从低到高。

Fig.7 Relative expression levels of wheat TaSWEET family and TaSUT family genes in different organs The legend color from bottom to top indicates gene expression from low to high.

图8 外源糖处理下小麦根中TaSWEET和TaSUT家族基因的相对表达量标“*”的表示与对照(CK)相比差异显著(p<0.05)。下同。

Fig.8 Relative expression levels of TaSWEET family and TaSUT family genes in response to exogenous sugar treatment in wheat roots “*” indicates significant (p<0.05) difference compared with the control (CK). The same as below.

图9 外源糖处理下小麦叶片中TaSWEET和TaSUT家族基因的相对表达量

Fig.9 Relative expression levels of TaSWEET family and TaSUT family genes in response to exogenous sugar treatment in wheat leaves

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小麦SWEET和SUT家族基因鉴定与表达分析

Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.)

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