西瓜蔗糖转运蛋白SUT家族的鉴定及其在果实发育和逆境响应中的表达分析

doi:10.3969/j.issn.1004-1524.20230188

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (1): 94-102.DOI: 10.3969/j.issn.1004-1524.20230188

西瓜蔗糖转运蛋白SUT家族的鉴定及其在果实发育和逆境响应中的表达分析

寿伟松(), 王铎, 沈佳, 许昕阳, 张跃建, 何艳军()

浙江省农业科学院蔬菜研究所,浙江杭州 310021

收稿日期:2023-02-17 出版日期:2024-01-25 发布日期:2024-02-18
作者简介:寿伟松(1972—),男,浙江绍兴人,硕士,副研究员,主要从事西甜瓜育种与栽培研究。E-mail: shouws@zaas.ac.cn
通讯作者: * 何艳军,E-mail: hyj1009@163.com
基金资助:
国家自然科学基金(32202478);浙江省自然科学基金(LY22C150010);浙江省农业新品种选育重大科技专项子课题任务(2021C02065-3-1-1)

Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

SHOU Weisong(), WANG Duo, SHEN Jia, XU Xinyang, ZHANG Yuejian, HE Yanjun()

Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-02-17 Online:2024-01-25 Published:2024-02-18

摘要/Abstract

摘要：

蔗糖转运蛋白(SUTs)作为蔗糖主动转运的主要载体,在分配同化物从源到库组织的转运中起着关键作用。尽管SUTs的特性及其生物学功能已在高等植物中得到了深入研究,但该基因家族尚未在西瓜中进行鉴定。本研究在西瓜基因组中共鉴定了4个ClSUT基因,并对其基因和蛋白结构、保守基序、亚细胞定位、染色体分布、进化关系、启动子元件等进行了全面分析。基因结构分析表明,亚族Ⅰ的ClSUT3和ClSUT4基因均只含有一个内含子。所有ClSUT均被预测定位在质膜中,它们均含有一个保守的MFS-2结构域,并且含有与之对应的5个保守基序。系统发育分析发现拟南芥、水稻和西瓜中的SUT分为5个亚族,西瓜ClSUT与拟南芥SUT的亲缘关系更近,被聚类在亚族Ⅰ、亚族Ⅱ和亚族Ⅴ。还在西瓜ClSUT基因的启动子中鉴定到一些与激素和逆境响应相关的作用元件。此外,转录组数据表明,西瓜ClSUT基因的表达对渗透和盐胁迫呈现多样性,很可能参与西瓜对这些逆境的响应。

关键词: 西瓜, 蔗糖转运蛋白SUT, 果实发育, 非生物胁迫, 表达分析

Abstract:

Sucrose transporter (SUTs) gene, as the main active transport vector of sucrose, plays a key role in the transport of allotment assimilates from source tissue to sink tissue. Although the characteristics and biological functions of SUTs have been well studied in higher plants, the SUT gene family has not been identified in watermelon. In this study, four ClSUT genes were identified in watermelon genome, and their gene and protein structure, conserved motifs, subcellular localization, chromosome distribution, evolutionary relationships and promoter cis-elements were comprehensively analyzed. Gene structure analysis showed that ClSUT3 and ClSUT4 grouped into subgroup Ⅰ and each only contained one intron. All ClSUT proteins were predicted to be localized in the plasma membrane. All ClSUT proteins contain a conserved MFS domain corresponding to five motifs. Phylogenetic analysis revealed that SUT proteins in Arabidopsis, rice and watermelon were divided into 5 subgroups. ClSUTs in watermelon have more closely relationship with SUT proteins in Arabidopsis and were clustered in subgroup Ⅰ, Ⅱ and Ⅴ. In addition, We also identified several functional cis-elements related to hormone and stress response in the watermelon ClSUT gene promoter. And transcriptome data indicated that watermelon ClSUT genes were variously expressed in response to osmotic and salt stress, which may be involved in watermelon responses to these stresses.

Key words: watermelon, sucrose transporter SUT, fruit development, abiotic stress, expression analysis

中图分类号:

S651

寿伟松, 王铎, 沈佳, 许昕阳, 张跃建, 何艳军. 西瓜蔗糖转运蛋白SUT家族的鉴定及其在果实发育和逆境响应中的表达分析[J]. 浙江农业学报, 2024, 36(1): 94-102.

SHOU Weisong, WANG Duo, SHEN Jia, XU Xinyang, ZHANG Yuejian, HE Yanjun. Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 94-102.

图/表 8

表1 西瓜ClSUT基因的基本信息

Table 1 The basic information of ClSUTs in watermelon

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome location	基因方向 Gene direction	氨基酸长度 Protein length/aa	等电点 Isoelectric point	蛋白分子量 Molecular weight/ku	亚细胞定位 Subcellular localization
ClSUT1	Cla97C08G155070.1	Chr08:23133250.. 23138158	-	507	9.36	54.98	质膜 Plasma membrane
ClSUT2	Cla97C09G183410.1	Chr09:36640778.. 36646361	+	604	5.67	64.78	质膜 Plasma membrane
ClSUT3	Cla97C11G215990.1	Chr11:13836471.. 13838050	-	495	8.55	53.15	质膜 Plasma membrane
ClSUT4	Cla97C11G216280.1	Chr11:16625895.. 16627473	+	495	8.84	53.25	质膜 Plasma membrane

图1 西瓜ClSUT的进化、保守基序和结构域分析 A,西瓜ClSUT进化分析;B,西瓜ClSUT保守基序分析;C,西瓜ClSUT保守结构域分析。

Fig.1 The phylogenetic, conserved motif, and domain analysis of ClSUTs in watermelon A, Phylogenetic tree of ClSUTs in watermelon; B, Conserved motifs of ClSUTs in watermelon; C, Conserved domains of ClSUTs in watermelon.

图2 西瓜ClSUT的基因结构

Fig.2 The gene structure of ClSUTs in watermelon

图3 拟南芥、水稻和西瓜SUT的系统发育分析

Fig.3 The phylogenetic analyses of SUTs in Arabidopsis, rice and watermelon

图4 西瓜ClSUT基因的染色体定位

Fig.4 The chromosomal localization of ClSUTs in watermelon

图5 西瓜ClSUT基因的启动子顺式元件分析

Fig.5 The cis-elements in the promoters of ClSUTs in watermelon

图6 西瓜ClSUT基因在西瓜PI296341-FR(A)和97103(B)果实不同发育时期的表达热图 DAP代表授粉后果实的天数。

Fig.6 Expression heatmap of ClSUTs in watermelon PI296341-FR(A) and 97103 (B) at different fruit developmental periods DAP represents days after pollination.

图7 西瓜ClSUT基因在渗透(A)和干旱(B)胁迫下的表达水平热图 CK,对照材料;OS,渗透胁迫下西瓜材料;Y34,干旱敏感西瓜材料;M20,抗旱西瓜材料。

Fig.7 The expression heatmap of ClSUT in different watermelon in response to osmotic (A) and drought (B) stress CK, Control; OS, Watermelon root tissue under osmotic stress; Y34, Non-drought resistant material; M20, Drought-resistant material.

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西瓜蔗糖转运蛋白SUT家族的鉴定及其在果实发育和逆境响应中的表达分析

Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

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