大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析

doi:10.3969/j.issn.1004-1524.20231003

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (9): 2031-2041.DOI: 10.3969/j.issn.1004-1524.20231003

大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析

欧晋稳¹^,²(), 张古文², 冯志娟², 王斌², 卜远鹏², 徐钰², 茹磊¹, 刘娜¹^,²^,^*(), 龚亚明²

1.浙江农林大学园艺科学学院,浙江杭州 311300
2.农产品质量安全危害因子与风险防控国家重点实验室,浙江省农业科学院蔬菜研究所,浙江杭州 310021

收稿日期:2023-08-22 出版日期:2024-09-25 发布日期:2024-09-30
作者简介:*刘娜,E-mail:ln200811@163.com
欧晋稳(1997—),女,广西宁明人,硕士研究生,研究方向为农艺与种业。E-mail:18377198030@163.com
通讯作者: *刘娜,E-mail:ln200811@163.com
基金资助:
浙江省重点研发计划(2021C02052);浙江省重点研发计划(2022C02016);浙江省“十四五”蔬菜新品种选育重大科技专项(2021C02065-6);农产品质量安全危害因子与风险防控国家重点实验室(2021DG700024-ZZ202206);浙江省基础公益研究计划(10404020421KH4402G)

Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

OU Jinwen¹^,²(), ZHANG Guwen², FENG Zhijuan², WANG Bin², BU Yuanpeng², XU Yu², RU Lei¹, LIU Na¹^,²^,^*(), GONG Yaming²

1. College of Horticultural Science, Zhejiang A&F University, Hangzhou 311300, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-08-22 Online:2024-09-25 Published:2024-09-30

摘要/Abstract

摘要：

海藻糖在植物代谢、生长发育和抗逆性中起着重要作用。海藻糖-6-磷酸磷酸酶(TPP)基因对海藻糖的生物合成至关重要。大豆是重要豆类作物,种子含有丰富的蛋白质和油脂,其TPP基因家族少有报道。为分析TPP基因在大豆中的结构和功能,利用生物信息学方法在大豆全基因组中筛选得到15个GmTPP基因。系统发育分析表明,这15个GmTPP可分为3个亚家族,每个GmTPP含有9~11个内含子,同一亚家族的GmTPP基因的内含子数目相同。顺式作用元件分析表明,GmTPP基因参与植物激素和环境胁迫反应。此外,还利用转录组数据研究这些GmTPP在不同组织中的表达模式以及对不同的非生物胁迫的表达特征。结果显示,GmTPP在大豆各个组织器官中均有特定的表达模式,在盐胁迫、干旱胁迫处理下表达不同。该研究不仅为揭示GmTPP基因家族在大豆海藻糖调控中的作用奠定了基础,也为利用TPP基因家族提高大豆抗逆性提供一定的参考价值。

关键词: 海藻糖-6-磷酸磷酸酶基因GmTPP, 大豆, 非生物胁迫, 表达分析

Abstract:

Trehalose plays an important role in plant metabolism, growth, development and stress resistance. The gene for trehalose-6-phosphate phosphatase (TPP) is crucial for the biosynthesis of trehalose. Soybeans, an important legume crop, are rich in protein and oil in their seeds, and there have been few reports on their TPP gene family. To analyze the structure and function of TPP genes in soybeans, 15 GmTPP genes were identified using bioinformatics methods across the entire soybean genome. Phylogenetic analysis indicated that these 15 GmTPP genes could be categorized into three subfamilies, with each GmTPP containing 9-11 introns, and the number of introns being the same within the same subfamily. Analysis of cis-acting elements suggested that GmTPP genes were involved in plant hormone and environmental stress responses. Furthermore, we utilized transcriptome data to study the expression patterns of these GmTPP genes in different tissues and their expression characteristics under various abiotic stresses. The results showed that GmTPP genes had specific expression patterns in all soybean tissues and organs and exhibited different expressions under salt and drought stress treatments. This study not only lays the foundation for revealing the role of the GmTPP gene family in the regulation of trehalose in soybeans but also provides some reference value for improving soybean stress resistance using the TPP gene family.

Key words: trehalose-6-phosphate phosphatase gene GmTPP, soybean, abiotic stress, expression analysis

中图分类号:

S565.1

欧晋稳, 张古文, 冯志娟, 王斌, 卜远鹏, 徐钰, 茹磊, 刘娜, 龚亚明. 大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析[J]. 浙江农业学报, 2024, 36(9): 2031-2041.

OU Jinwen, ZHANG Guwen, FENG Zhijuan, WANG Bin, BU Yuanpeng, XU Yu, RU Lei, LIU Na, GONG Yaming. Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2031-2041.

图/表 9

参考文献 41

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基因 Gene	基因序列号 Gene ID number	染色体 Chromosome	内含子数目 Number of introns	氨基酸数 Number of amino acids	分子量 Molecular mass/ku	等电点 Isoelectric point(pI)	亚细胞定位 Subcellular localization
GmTPP1	Glyma.04G103600	Gm04	9	371	41.438 67	9.35	叶绿体Chloroplast
GmTPP2	Glyma.04G119700	Gm04	11	340	37.565 88	5.52	叶绿体Chloroplast
GmTPP3	Glyma.04G237900	Gm04	9	367	41.772 01	8.86	叶绿体Chloroplast
GmTPP4	Glyma.05G179800	Gm05	10	277	32.010 92	8.89	过氧化物酶体Peroxisomal
GmTPP5	Glyma.06G104800	Gm06	9	370	41.444 76	9.46	叶绿体Chloroplast
GmTPP6	Glyma.06G126300	Gm06	10	367	42.046 28	9.09	叶绿体Chloroplast
GmTPP7	Glyma.08G137500	Gm08	10	290	33.448 61	8.83	过氧化物酶体Peroxisomal
GmTPP8	Glyma.09G231400	Gm09	11	389	43.419 69	8.54	细胞质Cytoplasmic
GmTPP9	Glyma.11G239300	Gm11	10	363	41.038 08	9.31	叶绿体Chloroplast
GmTPP10	Glyma.12G005200	Gm12	11	389	43.276 45	7.66	叶绿体Chloroplast
GmTPP11	Glyma.13G088300	Gm13	9	372	41.824 2	9.29	叶绿体Chloroplast
GmTPP12	Glyma.14G171700	Gm14	9	379	42.540 85	9.47	叶绿体Chloroplast
GmTPP13	Glyma.16G025600	Gm16	9	203	23.115 63	6.53	细胞质Cytoplasmic
GmTPP14	Glyma.17G138700	Gm17	11	362	41.039 38	5.79	细胞核Nuclear
GmTPP15	Glyma.18G018100	Gm18	11	365	41.200 30	9.31	叶绿体Chloroplast

基因 Gene	基因序列号 Gene ID number	染色体 Chromosome	内含子数目 Number of introns	氨基酸数 Number of amino acids	分子量 Molecular mass/ku	等电点 Isoelectric point(pI)	亚细胞定位 Subcellular localization
GmTPP1	Glyma.04G103600	Gm04	9	371	41.438 67	9.35	叶绿体Chloroplast
GmTPP2	Glyma.04G119700	Gm04	11	340	37.565 88	5.52	叶绿体Chloroplast
GmTPP3	Glyma.04G237900	Gm04	9	367	41.772 01	8.86	叶绿体Chloroplast
GmTPP4	Glyma.05G179800	Gm05	10	277	32.010 92	8.89	过氧化物酶体Peroxisomal
GmTPP5	Glyma.06G104800	Gm06	9	370	41.444 76	9.46	叶绿体Chloroplast
GmTPP6	Glyma.06G126300	Gm06	10	367	42.046 28	9.09	叶绿体Chloroplast
GmTPP7	Glyma.08G137500	Gm08	10	290	33.448 61	8.83	过氧化物酶体Peroxisomal
GmTPP8	Glyma.09G231400	Gm09	11	389	43.419 69	8.54	细胞质Cytoplasmic
GmTPP9	Glyma.11G239300	Gm11	10	363	41.038 08	9.31	叶绿体Chloroplast
GmTPP10	Glyma.12G005200	Gm12	11	389	43.276 45	7.66	叶绿体Chloroplast
GmTPP11	Glyma.13G088300	Gm13	9	372	41.824 2	9.29	叶绿体Chloroplast
GmTPP12	Glyma.14G171700	Gm14	9	379	42.540 85	9.47	叶绿体Chloroplast
GmTPP13	Glyma.16G025600	Gm16	9	203	23.115 63	6.53	细胞质Cytoplasmic
GmTPP14	Glyma.17G138700	Gm17	11	362	41.039 38	5.79	细胞核Nuclear
GmTPP15	Glyma.18G018100	Gm18	11	365	41.200 30	9.31	叶绿体Chloroplast

大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析

Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

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