关键词: 黑李, 免疫诱导剂, 蛋白质组学, GO分类, 代谢途径分析

Abstract: The plum variety Friar plum was used as test material. Black plum leaves were treated with immunity inducer Baokangling No.1. Proteomic analysis and determination of physiological and biochemical indexes of treated leaves were carried out. The results showed that area, thickness, chlorophyll content and PPO activities of black plum leaf treated with immunity inducer Baokangling No.1 all increased. The cross section of blade showed that the palisade tissue, spongy tissue and lower epidermis were obviously thickened after treatment with immunity inducer, which was probably responsible for leaf thickening and increased chlorophyll content. Proteomic analysis showed that a total of 157 differentially expressed proteins were screened, including 75 up-regulated proteins (up to 1.5 fold and P<0.05) and 82 down-regulated proteins (down 1.5 fold and P<0.05).GO classification and KEGG pathway analysis showed that differentially up-regulated protein (or genes) were enriched and assigned to the chloroplast, chloroplast membrane and chloroplast matrix function, whereas differentially down regulated proteins (or genes) were enriched and assigned to chloroplast matrix, the chloroplast membrane and photosystem Ⅰ. Both of them were involved in photosynthesis. Taken together, the immunity inducer Baokangling No.1 may promote the growth of black plum leaves via regulating the expression of proteins related to photosynthesis.

Key words: black plum, immunity inducer, proteomics, GO classification, pathway analysis