›› 2009, Vol. 21 ›› Issue (6): 0-564.

• 论文 •    

湖羊高繁殖力候选基因ESR的研究

董文艳1,陈阿琴2,王争光1,俞颂东1,*
  

  1. 1 浙江大学 动物科学学院,浙江 杭州 310029;2上海海洋大学 水产与生命学院,上海201306
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-25 发布日期:2009-11-25

Estrogen receptor as a candidate gene for prolificacy of Hu sheep

DONG Wen-yan;CHEN A-qin;WANG Zheng-guang;YU Song-dong;*   

  1. 1 College of Animal Science,Zhejiang University,Hangzhou 310029,China;2 College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-25 Published:2009-11-25

摘要:

利用 PCR-SSCP技术对高繁殖力绵羊品种湖羊雌激素受体(estrogen receptor,ESR)基因第一外显子的部分序列进行单核苷酸多态性研究。结果表明,湖羊ESR基因中存在 3 种基因型 AA,BB,AB,且A等位基因频率为0.554,B 等位基因频率为0.446。经测序,BB型与AA型相比在外显子 1 第 363 位发生 1 处C→G的碱基突变。据产羔数统计,AB 基因型和 BB 基因型的湖羊产羔数分别比 AA 基因型多0.98只(P<0.01)、1.47只(P<0.01)。说明ESR基因可能是控制湖羊多羔性能的一个主效基因或与之存在紧密遗传连锁的一个标记。



关键词: 湖羊, 高繁殖力, 候选基因, 雌激素受体基因, PCR-SSCP

Abstract: Single nucleotide polymorphism in exon 1 of the estrogen receptor (ESR) gene was detected by PCR-SSCP in high fecundity Hu sheep breed. The results indicated that there were three genotypes (AA,BB,AB) in Hu sheep and the frequency of allele A and B was 0.554 and 0.446,respectively. Comparison with the AA genotype,sequencing revealed a C→G mutation at 363 bp of exon 1 of ESR gene in the BB genotype. The Hu sheep with AB genotype or BB genotype had 0.98 and 1.47 more lambs than those with AA genotype,respectively. These results showed that the estrogen receptor locus was a major gene that controlled the prolificacy in Hu sheep or a mark in close linkage with such a gene.

Key words: Hu sheep(Ovis aries), prolificacy, candidate gene, estrogen receptor gene, PCR-SSCP