LED绿光对小贯小绿叶蝉取食行为的干扰

doi:10.3969/j.issn.1004-1524.20240268

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (9): 2051-2059.DOI: 10.3969/j.issn.1004-1524.20240268

LED绿光对小贯小绿叶蝉取食行为的干扰

乔利¹(), 陈磊¹, 蒋月丽², 周洲¹, 耿书宝¹, 王丽娟³^,^*()

1.信阳农林学院农学院,河南信阳 464000
2.河南省农业科学院植物保护研究所,河南郑州 450002
3.中国林业科学研究院林业研究所,北京 100091

收稿日期:2024-03-27 出版日期:2024-09-25 发布日期:2024-09-30
作者简介:*王丽娟,E-mail: wlj307@caf.ac.cn
乔利(1980—),女,河南鲁山人,博士,副教授,主要从事昆虫生态与害虫防治研究。E-mail: 2017180004@xyafu.edu.cn
通讯作者: *王丽娟,E-mail: wlj307@caf.ac.cn
基金资助:
国家重点研发计划(2017YFD0200907);国家自然科学基金(32001902);河南省重点研发与推广专项(222102110109);信阳农林学院科技创新团队(XNKJTD-007)

Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda

QIAO Li¹(), CHEN Lei¹, JIANG Yueli², ZHOU Zhou¹, GENG Shubao¹, WANG Lijuan³^,^*()

1. School of Agronomy, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China
2. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-03-27 Online:2024-09-25 Published:2024-09-30

摘要/Abstract

摘要：

小贯小绿叶蝉是我国茶区普遍发生的害虫之一,其为害严重影响了茶叶的产量和品质。为减少化学农药带来的残留,探索茶园叶蝉防治新方法,采用LED绿光(波长520~525 nm)干扰其取食过程,并通过刺吸电位图谱(EPG)仪记录分析不同光照强度和照射时间对小贯小绿叶蝉取食行为的影响。结果表明,小贯小绿叶蝉取食过程中所产生的波型有NP波、A波、C波、E波、S波、F波和R波,其中NP波占比最大。与自然光(对照)相比,LED绿光对小贯小绿叶蝉取食行为有明显的抑制作用,相同时间内,光照强度越大,刺吸百分比越低,刺吸次数越多,对小贯小绿叶蝉取食行为的干扰越明显。该研究结果可为灯光防治茶园叶蝉的应用提供技术支持。

关键词: LED绿光, 取食行为, 小贯小绿叶蝉, 刺吸电位图谱

Abstract:

Empoasca onukii Matsuda is one of the common pests in tea areas in China, and its harm seriously affects the yield and quality of tea. In order to reduce the residues of chemical pesticides and explore new control methods of leafhoppers in tea garden, LED green light (wavelength 520-525 nm) was used to interfere the feeding process of E. onukii, which was sensitive to light, the effects of light intensity and irradiation time on the feeding behavior of E. onukii were analyzed by means of electrical penetration graph (EPG) recording. The results showed that, the waveform produced by the feeding process of E. onukii were NP wave, A wave, C wave, E wave, S wave, F wave and R wave, among which NP wave accounted for the largest proportion. Compared with natural light (control), LED green light significantly inhibited the feeding behavior of E. onukii. At the same time, the higher the light intensity, the lower the percentage of spiking, and the more stinging and sucking times, the more obvious interference to the feeding behavior of E. onukii. The results of this study could provide technical support for the application of light prevention and control of leafhopper in tea garden.

Key words: LED green light, feeding behavior, Empoasca onukii Matsuda, electrical penetration graph (EPG)

中图分类号:

S433.3

乔利, 陈磊, 蒋月丽, 周洲, 耿书宝, 王丽娟. LED绿光对小贯小绿叶蝉取食行为的干扰[J]. 浙江农业学报, 2024, 36(9): 2051-2059.

QIAO Li, CHEN Lei, JIANG Yueli, ZHOU Zhou, GENG Shubao, WANG Lijuan. Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2051-2059.

图/表 6

参考文献 30

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光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.01±0.47 a	6.00±0.80 a	4.98±1.67 b	11.16±1.42 b	5.65±1.98 ab	4.57±1.47 ab	85.63±2.40 c
120	1.14±0.41 b	5.50±1.11 ab	6.44±1.49 b	10.52±0.90 b	6.19±2.94 a	1.52±1.12 c	88.70±2.56 c
160	1.12±0.66 b	5.05±0.94 ab	5.13±0.57 b	8.23±0.67 c	4.20±2.11 abc	1.35±0.22 c	94.92±3.55 b
200	1.20±0.34 b	3.90±0.83 bc	4.83±0.51 b	5.97±1.29 d	2.60±0.51 bc	1.79±1.21 c	99.71±2.53 b
240	0.97±0.36 b	2.86±0.83 c	1.78±0.31 c	3.90±0.42 e	2.18±0.73 c	2.94±1.38 bc	105.38±2.11 a
对照CK	1.61±0.69 ab	5.38±1.35 ab	8.54±1.72 a	15.86±0.62 a	5.05±2.81 abc	5.15±0.93 a	78.41±5.94 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.01±0.47 a	6.00±0.80 a	4.98±1.67 b	11.16±1.42 b	5.65±1.98 ab	4.57±1.47 ab	85.63±2.40 c
120	1.14±0.41 b	5.50±1.11 ab	6.44±1.49 b	10.52±0.90 b	6.19±2.94 a	1.52±1.12 c	88.70±2.56 c
160	1.12±0.66 b	5.05±0.94 ab	5.13±0.57 b	8.23±0.67 c	4.20±2.11 abc	1.35±0.22 c	94.92±3.55 b
200	1.20±0.34 b	3.90±0.83 bc	4.83±0.51 b	5.97±1.29 d	2.60±0.51 bc	1.79±1.21 c	99.71±2.53 b
240	0.97±0.36 b	2.86±0.83 c	1.78±0.31 c	3.90±0.42 e	2.18±0.73 c	2.94±1.38 bc	105.38±2.11 a
对照CK	1.61±0.69 ab	5.38±1.35 ab	8.54±1.72 a	15.86±0.62 a	5.05±2.81 abc	5.15±0.93 a	78.41±5.94 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.68±0.78 ab	8.62±1.11 a	8.46±2.23 b	26.06±2.34 ab	10.04±1.23 a	8.74±1.95 ab	175.41±5.21 d
120	1.94±0.38 b	7.48±0.86 ab	8.28±1.73 b	22.82±2.98 bc	8.67±1.84 ab	6.70±2.27 b	184.12±2.85 c
160	2.25±0.62 ab	6.84±1.07 ab	6.24±0.56 c	20.10±2.74 c	7.16±1.77 bc	6.57±1.45 b	190.85±3.70 bc
200	1.89±0.33 b	5.69±1.50 bc	6.93±0.91 bc	15.70±3.11 d	6.09±1.40 c	6.08±2.35 b	197.63±7.66 b
240	1.70±0.27 b	4.42±0.93 c	4.63±0.42 d	8.57±0.96 e	4.94±0.62 c	6.35±1.70 b	209.40±2.64 a
对照CK	3.02±1.07 a	8.09±1.57 a	11.13±0.66 a	27.28±2.92 a	6.03±1.03 c	11.39±2.34 a	173.07±3.53 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.68±0.78 ab	8.62±1.11 a	8.46±2.23 b	26.06±2.34 ab	10.04±1.23 a	8.74±1.95 ab	175.41±5.21 d
120	1.94±0.38 b	7.48±0.86 ab	8.28±1.73 b	22.82±2.98 bc	8.67±1.84 ab	6.70±2.27 b	184.12±2.85 c
160	2.25±0.62 ab	6.84±1.07 ab	6.24±0.56 c	20.10±2.74 c	7.16±1.77 bc	6.57±1.45 b	190.85±3.70 bc
200	1.89±0.33 b	5.69±1.50 bc	6.93±0.91 bc	15.70±3.11 d	6.09±1.40 c	6.08±2.35 b	197.63±7.66 b
240	1.70±0.27 b	4.42±0.93 c	4.63±0.42 d	8.57±0.96 e	4.94±0.62 c	6.35±1.70 b	209.40±2.64 a
对照CK	3.02±1.07 a	8.09±1.57 a	11.13±0.66 a	27.28±2.92 a	6.03±1.03 c	11.39±2.34 a	173.07±3.53 d

光照强度 Light intensity/lx	A波 A wave	S波 S wave	C波 C wave	E波 E wave	F波 F wave	R波 R wave	NP波 NP wave
80	2.55±0.35 b	9.80±0.64 b	10.78±1.14 b	35.42±1.19 b	12.72±2.32 ab	10.96±1.10 bc	277.78±3.46 c
120	2.06±0.53 b	9.19±0.79 bc	11.31±0.42 b	31.02±5.93 b	11.95±4.48 ab	9.89±1.57 c	284.60±8.02 bc
160	2.16±0.34 b	8.48±0.35 c	9.19±0.33 c	23.06±1.63 c	13.29±3.29 ab	12.76±1.19 b	291.08±4.04 b
200	2.06±0.20 b	8.44±0.36 c	9.24±0.80 c	21.09±1.97 c	10.34±1.94 b	14.42±1.55 a	294.41±3.92 b
240	1.95±0.29 b	4.86±0.40 d	6.04±1.15 d	8.63±0.66 d	9.23±1.66 b	10.56±2.52 bc	318.75±2.20 a
对照CK	4.34±0.59 a	13.05±1.68 a	15.07±1.36 a	46.27±8.08 a	15.05±1.06 a	15.16±1.60 a	251.06±11.84 d

LED绿光对小贯小绿叶蝉取食行为的干扰

Interference of LED green light on the feeding behavior of Empoasca onukii Matsuda

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