烤烟品种中川208和CV87根际细菌群落分析

doi:10.3969/j.issn.1004-1524.20240631

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (7): 1512-1520.DOI: 10.3969/j.issn.1004-1524.20240631

烤烟品种中川208和CV87根际细菌群落分析

耿锐梅¹(), 赵清海², 曹长代³, 李峰⁴, 王大海², 贺鹏霖⁴, 刘洋², 李军民³, 徐蕊⁴, 宋志美⁵, 胡海洲⁵^,^*(), 张玉¹^,^*()

1.中国农业科学院烟草研究所,山东青岛 266101
2.山东日照烟草有限公司,山东日照 276826
3.山东潍坊烟草有限公司,山东潍坊 261205
4.山东临沂烟草有限公司,山东临沂 276003
5.青岛中烟种子有限责任公司,山东青岛 266100

收稿日期:2024-07-12 出版日期:2025-07-25 发布日期:2025-08-20
作者简介:耿锐梅(1980—),女,云南宣威人,博士,助理研究员,主要从事烟草遗传育种研究。E-mail:gengruimei@caas.cn
通讯作者: ^*张玉,E-mail:zhangyu02@caas.cn;
胡海洲,E-mail:huhaizhou@caas.cn
基金资助:
中国烟草总公司“揭榜挂帅”项目(110202103014);青岛中烟种子有限责任公司研发项目(ycskY202212102-c1);中国烟草总公司山东省公司重点项目(202207);中国农业科学院科技创新工程(ASTIP-TRIC01)

Analysis of rhizosphere bacterial flora of flue-cured tobacco varieties Zhongchuan 208 and CV87

GENG Ruimei¹(), ZHAO Qinghai², CAO Changdai³, LI Feng⁴, WANG Dahai², HE Penglin⁴, LIU Yang², LI Junmin³, XU Rui⁴, SONG Zhimei⁵, HU Haizhou⁵^,^*(), ZHANG Yu¹^,^*()

1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China
2. Shandong Rizhao Tobacco Company Ltd., Rizhao 276826, Shandong, China
3. Shandong Weifang Tobacco Company Ltd., Weifang 261205, Shandong, China
4. Shandong Linyi Tobacco Company Ltd., Lingyi 276003, Shandong, China
5. Qingdao Zhongyan Seed Co., Ltd., Qingdao 266100, China

Received:2024-07-12 Online:2025-07-25 Published:2025-08-20

摘要/Abstract

摘要：

根际微生物易受到植物生长时期和基因型的影响。为探究不同烤烟品种根际细菌菌群的差异及其相互作用,以烤烟品种中川208和CV87为对象,基于16S rRNA测序技术获得根际细菌菌群序列,利用多样性分析、组成型分析等方法,对不同生长时期两个品种的烟草根际细菌菌群进行详细比较与分析。研究发现,基因型和生育期影响根际细菌的群落结构和细菌间的相互关系。中川208的根际细菌群落多样性在初花期最高,而CV87是在成熟期最高。中川208和CV87的根际细菌物种间分别有435和282个edge(即相关性数据),说明中川208根际细菌的类群及其物种间的互作较CV87更加丰富和复杂。在中川208的团棵期、旺长期,固氮弧菌属(Azoarcus)的丰度较高,有助于中川208更有效地获取氮素。在CV87的根际细菌中,Ramlibacter在这两个生长期也显示出较高的丰度,可激活CV87植株的抗氧化系统,增强植株对病害的抵抗力。研究结果可为深入理解烟草根际微生物的组成和进一步深入揭示烟草根际微生物的互作机制提供基础数据和理论参考。

关键词: 烤烟, 根际微生物, 多样性分析, 组成型分析

Abstract:

The root microbiota is susceptible to the influence of the plant growth stages and genotypes. To explore the differences in the rhizosphere bacterial flora of flue-cured tobacco varieties and their interactions, the new tobacco variety Zhongchuan 208 and CV87 were used as test materials in the present study. By utilizing 16S rRNA sequencing, sequences of the rhizosphere bacterial flora were obtained. Through methods such as diversity and compositional analysis, a detailed comparison of the rhizosphere bacterial flora of two flue-cured tobacco varieties at various growth stages was conducted. It was revealed that genotypes and growth stages affected the structure of rhizosphere bacterial communities and the relationships between bacteria. For Zhongchuan 208, the highest diversity was observed during the early blooming stage, while for CV87, the highest diversity was found during the maturity stage. There were 435 and 282 edges (correlation data) between species in the rhizosphere bacterial flora of Zhongchuan 208 and CV87, respectively, indicating that the interactions within rhizosphere bacterial flora of Zhongchuan 208 were richer and more complex than those of CV87. During the rosette and vigorous growth stages, the genus Azoarcus in the rhizosphere of Zhongchuan 208 was relatively abundant, which may help the plant obtain nitrogen through nitrogen fixation, thereby promoting the plant growth. In the rhizosphere of CV87, Ramlibacter maintained a relatively high abundance during the rosette and vigorous growth stages, which may participate in the activation of the antioxidant system of plant to enhance the resistance to diseases and other processes. The findings provided both data and theoretical basis for a deeper understanding of the compositional patterns and the interaction mechanisms of the rhizosphere microbiome of flue-cured tobacco.

Key words: flue-cured tobacco, rhizosphere microbiome, diversity analysis, taxonomic composition

中图分类号:

S572

耿锐梅, 赵清海, 曹长代, 李峰, 王大海, 贺鹏霖, 刘洋, 李军民, 徐蕊, 宋志美, 胡海洲, 张玉. 烤烟品种中川208和CV87根际细菌群落分析[J]. 浙江农业学报, 2025, 37(7): 1512-1520.

GENG Ruimei, ZHAO Qinghai, CAO Changdai, LI Feng, WANG Dahai, HE Penglin, LIU Yang, LI Junmin, XU Rui, SONG Zhimei, HU Haizhou, ZHANG Yu. Analysis of rhizosphere bacterial flora of flue-cured tobacco varieties Zhongchuan 208 and CV87[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1512-1520.

图/表 6

图1 不同生育期各烟草品种的根际细菌菌群组成 A,根际细菌菌群的偏最小二乘法判别分析(PLS-DA),括号中的数字为整体差异的解释率;B,中川208根际细菌菌群的辛普森指数(Simpson index);C,CV87根际细菌菌群的辛普森指数(Simpson index)。PC1,第1主成分;PC2,第2主成分。箱线图中的框内横线表示中位数,上下边缘分别代表上下四分位数,边缘上的延长线在无异常值时至极值,但最长不超过1.5倍上下四分位数的分布区间。柱上无相同字母的表示差异显著(P<0.05)。

Fig.1 Rhizosphere bacterial flora of different tobacco genotypes at different growth period A, Partial least squares discriminant analysis (PLS-DA) of the rhizosphere bacterial flora based on genotype and growth period, with the percentage of explained variance in overall differences shown in parentheses. B, The Simpson index of the rhizosphere bacterial flora for Zhongchuan 208. C, The Simpson index of the rhizosphere bacterial flora for CV87. PC1, The first principle component; PC2, The second principle component. The horizontal line inside the box in the boxplot represents the median, and the upper and lower edges represent the upper and lower quartiles, respectively. The whiskers on the edges extend to the extreme values when there are no outliers, but at most to 1.5 times the range of the upper and lower quartiles. Bars marked without the same letters indicate significant difference at P<0.05.

图2 中川208(左)和CV87(右)根际细菌群落物种组成在属水平上的韦恩图

Fig.2 Venn diagram of rhizosphere bacterial species composition (genus level) of Zhongchuan 208 and CV87

图3 中川208和CV87根际细菌在门水平(上)和属水平(下)的物种组成 Proteobacteria,变形菌门;Bacteroidota,拟杆菌门;Actinobacteriota,放线菌门;Acidobacteriota,酸杆菌门;Firmicutes,厚壁菌门;Verrucomicrobiota,疣微菌门;Patescibacteria,髌骨菌门;Chloroflexi,绿弯菌门;Gemmatimonadota,芽单胞菌门;Cyanobacteria,蓝菌门;others,其他;Novosphingobium,新鞘氨醇杆菌属;Sphingobium,鞘脂菌属;Sphingomonas,鞘氨醇单胞菌属;norank_f_Microscillaceae,微颤蓝细菌科未分类属;Pseudomonas,假单胞菌属;Bacillus,芽孢杆菌属;Mucilaginibacter,黏液杆菌属;Arthrobacter,节杆菌属。

Fig.3 Rhizosphere bacterial species composition at the phylum level (up) and genus level (down) of Zhongchuan 208 and CV87

图4 不同生育期中川208(上)和CV87(下)根际细菌群落的物种差异 Bacillus,芽孢杆菌属;norank_f_Microscillaceae,微颤蓝细菌科未分类属;Sphingomonas,鞘氨醇单胞菌属;Mucilaginibacter,黏液杆菌属;Pseudomonas,假单胞菌属;Streptomyces,链霉菌属;Arthrobacter,节杆菌属;Massilia,马赛菌属;norank_f_Chitinophagaceae,噬几丁质菌科未定名属;Devosia,德沃斯氏菌属;Novosphingobium,新鞘氨醇杆菌属;Sphingobium,鞘脂菌属;Flavobacterium,黄杆菌属。

Fig.4 Multi-species differences of rhizosphere bacterial species of Zhongchuan 208 (up) and CV87 (down) at different growth stages

图5 中川208(A)和CV87(B)的根际细菌组别 Acidobacteriota,酸杆菌门;Actinobacteriota,放线菌门;Bacteroidota,拟杆菌门;Cyanobacteria,蓝菌门;Chloroflexi,绿弯菌门;Firmicutes,厚壁菌门;Gemmatimonadota,芽单胞菌门;Myxococcota,黏细菌门;Nitrospirota,硝化螺旋菌门;Patescibacteria,髌骨细菌门;Protebocteria,变形菌门;Verrucomicrobiota,疣微菌门;Nocardioides,诺卡菌属;Streptomyces,链霉菌属;Paenarthrobacter,拟节杆菌属;Arthrobacter,节杆菌属;Flavisolibacter,黄色土源菌属;Pedobacter,土地杆菌属;Mucilaginibacter,黏液杆菌属;Chitinophaga,噬几丁质菌属;Bryobacter,热酸菌属;Bacillus,芽孢杆菌属;Gemmatimonas,芽单胞菌属;Bradyrhizobium,慢生根瘤菌属;Pseudomonas,假单胞菌属;Azoarcus,固氮弧菌属;Mesorhizobium,中慢生根瘤菌属;Devosia,德沃斯氏菌属;Massilla,马赛菌属;Sphingobium,鞘脂菌属;Sphingomonas,鞘氨醇单胞菌属;Novosphingobium,新鞘氨醇杆菌属;Sphingobacterium,鞘氨醇杆菌属;Flavobacterium,黄杆菌属;Bacteroides,拟杆菌属;Bacillus,芽孢杆菌属;Variovorax,贪噬菌属。图上每个圈代表一个属水平上的物种,不同的颜色代表不同的门水平归属,圈上的标记即为物种名称,其中未能明确分类的物种(比如norank物种和unclassfide物种)未展示名称。物种之间的连线代表两者具有相关性,连线的粗细代表相关性的高低,连线的颜色代表相关性类型,红色代表正相关,绿色代表负相关。

Fig.5 Root microbiome of Zhongchuan 208 (A) and CV87 (B) Each circle in the graph represents a species at the genus level, with different colors representing different phylum-level affiliations. The labels on the circles are the names of the species, and species that cannot be clearly classified (such as norank species and unclassified species) are not shown with names. The lines between species represent correlations, with the thickness of the lines indicating the strength of the correlation, and the color of the lines representing the type of correlation. The red lines indicate positive correlations and the green lines indicate negative correlations.

表1 典型代表物种的丰度变化

Table 1 Changes of abundance in typical representative species

物种 Species	中川208根际样品中各物种的丰度 Species abundance in rhizosphere samples of Zhongchuan 208				CV87根际样品中各物种的丰度 Species abundance in rhizosphere samples of CV87
物种 Species	ZC1	ZC2	ZC3	ZC4	CV1	CV2	CV3	CV4
固氮弧菌属Azoarcus	827	879	491	627	291	91	80	106
芽孢杆菌属Bacillus	987	210	6 886	1 783	244	221	1 357	498
Dongia	1 056	923	423	390	1 256	619	237	190
Candidatus_Solibacter	381	299	440	786	476	379	414	345
黏液杆菌属Mucilaginibacter	784	820	1 619	3 084	1 034	608	1 564	1 215
Occallatibacter	271	263	653	911	566	359	706	415
Ramlibacter	358	628	190	257	525	646	244	288
Aquicella	1 121	801	511	672	951	613	334	340
黄杆菌属Flavobacterium	433	586	550	162	744	2 589	2 270	806
土地杆菌属Pedobacter	68	310	952	171	138	962	876	567
新鞘氨醇杆菌属Novosphingobium	2 772	2 019	2 036	1 225	1 997	5 400	4 724	2 523
贪噬菌属Variovorax	116	183	263	169	116	824	459	205
鞘氨醇单胞菌属Sphingomonas	1 188	2 015	1 603	4 427	1 365	2 916	1 868	3 234
马赛菌属Massilia	1 854	1 069	642	650	567	957	768	911
芽单胞菌属Gemmatimonas	357	812	362	933	420	661	508	898

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烤烟品种中川208和CV87根际细菌群落分析

Analysis of rhizosphere bacterial flora of flue-cured tobacco varieties Zhongchuan 208 and CV87

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