植物乳杆菌ZJQ的鉴定及其细菌素的生化特性

›› 2010, Vol. 22 ›› Issue (2): 0-228.

• 论文 •

植物乳杆菌ZJQ的鉴定及其细菌素的生化特性

鲁渊,宋达峰,顾青^*

浙江工商大学食品与生物工程学院,浙江杭州 310035

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25

Identification of Lactobacillus plantarum ZJQ and characteristics of bacteriocin ZJQ

LU Yuan;SONG Da-feng;GU Qing*

College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

摘要/Abstract

摘要： 从婴儿粪便中分离得到一株能产抑菌物质的乳酸菌菌株。形态学、生理生化和16S rDNA序列分析表明该菌为一株革兰氏阳性植物乳杆菌(Lactobacillus plantarum ZJQ)。胰蛋白酶和蛋白酶K等酶学试验分析证明该菌所产抑菌物质为细菌素,初步纯化表明,该细菌素经100℃处理后仍有80%抑菌活性,具有良好的热稳定性；可抑制藤黄微球菌、金黄色葡萄球菌、大肠杆菌、沙门氏菌等,具有较广的抑菌谱。

关键词: 植物乳杆菌, 细菌素, 抑菌物质

Abstract: A bacteriocin-producing strain was isolated from infant feces. With the morphological observation, physiological and biochemical characteristics and sequencing of the 16S rDNA, this strain was identified to be a gram\|positive Lactobacillus plantarum. Bacteriocin activity was degraded by proteolytic enzymes (trypsin, proteinase K etc.), and it was stable at high temperatures (100℃, 30 min), in a range of pH 2-4. The bacteriocin showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Salmonella species.

Key words: Lactobacillus plantarum, bacteriocin, antibacterial substances

鲁渊;宋达峰;顾青*. 植物乳杆菌ZJQ的鉴定及其细菌素的生化特性[J]. , 2010, 22(2): 0-228.

LU Yuan;SONG Da-feng;GU Qing*. Identification of Lactobacillus plantarum ZJQ and characteristics of bacteriocin ZJQ[J]. , 2010, 22(2): 0-228.

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植物乳杆菌ZJQ的鉴定及其细菌素的生化特性

Identification of Lactobacillus plantarum ZJQ and characteristics of bacteriocin ZJQ

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