金珠果梨S基因型的确定

›› 2010, Vol. 22 ›› Issue (4): 0-428.

• 论文 •

金珠果梨S基因型的确定

袁德义,谭晓风,曾艳玲,邹锋,谢鹏,张琳

中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙 410004

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-25 发布日期:2010-07-25

Identification of S-genotype of Jinzhuguo pear

YUAN De-yi;TAN Xiao-feng;ZENG Yan-ling;ZOU Feng;XIE Peng;ZHANG Lin

Key Lab of Non-wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-25 Published:2010-07-25

摘要/Abstract

摘要： 大多数梨属于配子体自交不亲和果树,自然授粉结实率低、品质差。通过确定梨品种S基因型可寻找简便快速克服自交不亲和性的方法。金珠果梨是从野生砂梨中选育出来的新种质。以金珠果梨幼叶为试验材料,对其基因组DNA进行S基因特异扩增、克隆、DNA序列测定及生物信息学分析等,结果表明：金珠果梨S基因型为S₃S₁₉。

关键词: Jinzhuguo pear, self-incompatibility, S-genotype

Abstract: Pear is a typical self-incompatible fruit tree. Its yield is low and quality is bad under natural pollination conditions. The approach of overcoming self-incompatibility, increasing production and improving quality could be found through identification of pear S genotype. The new germplasm Jinzhuguo is selected from wild Pyrus pyrifolia(Burm.f.)Nakai. Young leaves of Jinzhuguo pear was selected as materials, and special PCR, cloning, DNA sequencing and analysis of biological information were carried out with its genomic DNA. As a result, the S-genotype of Jinzhuguo pear was identified as S₃ S₁₉.

Key words: Jinzhuguo pear, self-incompatibility, S-genotype

袁德义;谭晓风;曾艳玲;邹锋;谢鹏;张琳. 金珠果梨S基因型的确定[J]. , 2010, 22(4): 0-428.

YUAN De-yi;TAN Xiao-feng;ZENG Yan-ling;ZOU Feng;XIE Peng;ZHANG Lin. Identification of S-genotype of Jinzhuguo pear[J]. , 2010, 22(4): 0-428.

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Identification of S-genotype of Jinzhuguo pear

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