关键词: 水稻纹枯病, 立枯丝核菌, T-DNA突变体, 抗性资源

Abstract: Isolated from Nipponbare T-DNA mutant library, 2 576 rice mutant lines were used to identify resistance resources to sheath blight (SB). Most of them were high-susceptible to rice SB by broadcasting the mixture of rice hulls and unhulled grains infested with the pathogen, and only 36 lines reached moderate resistant or resistant level, 180 lines were moderately susceptible. Further identification was carried out among 216 lines whose resistances were below the moderate level using insertion short toothpick infected with pathogen, the results showed there were 18 lines more resistant than the others among secondary screening lines. Final screening with micro-chamber inoculation to 18 lines in greenhouse was carried out and the results indicated there was no significant difference among different mutant lines, but there are 14 mutants whose resistant level were significantly higher than Nipponbare．

Key words: rice sheath blight, Rhizoctonia solani, T-DNA mutants, resistance resources