›› 2010, Vol. 22 ›› Issue (4): 0-447.

• 论文 •    

番茄黄化曲叶病毒病抗性鉴定与遗传分析

郑积荣1,王慧俐2   

  1. 1杭州市农业科学研究院, 浙江 杭州310024;2杭州万向职业技术学院,浙江 杭州310023
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-25 发布日期:2010-07-25

Resistance identification and genetic analysis of Tomato yellow leaf curl virus(TYLCV)

ZHENG Ji-rong;WANG Hui-li   

  1. 1Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 2College of Wanxiang Vocational Technology, Hangzhou 310023, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-25 Published:2010-07-25

摘要: 以抗病亲本P2(161-2-3-3-5-2-2-10)和感病亲本P1(9905-3-4-3-2-1-4-1-0) 为材料,通过有性杂交、自交和回交,获得P1,P2,F1,F2,BC1P1和BC1P2等6个世代的番茄材料,并以番茄黄化曲叶病毒为毒源, 进行苗期人工接种和大田自然发病鉴定,以探讨抗源对黄化曲叶病毒病的抗性遗传规律。结果表明,在接种20 d左右,感病植株开始发病,接种30 d后感病植株已充分发病;田间植株在开花结果期时,感病植株大部分花穗凋萎,结果稀少。各世代抗感植株均表现出规律性分布;卡方测验结果表明, 抗源材料P2对黄化曲叶病毒的抗性表现为单基因显性。

关键词: 番茄黄化曲叶病毒病, 人工接种, 自然发病鉴定, 抗性遗传

Abstract: Parent P2 (161-2-3-3-5-2-2-10) resistant to Tomato yellow leaf curl virus(TYLCV) and parent P1 (9905-3-4-3-2-1-4-1-0) susceptible to TYLCV were selected as parent materials and P1, P2, F1 (P1×P2), F2, and BC1P1 (F1×P1) and BC1P2 (F1×P2) were obtained through sexual hybridization, self cross and backcross. In order to explore the regularity of inheritance of resistance to TYLCV of materials above, TYLCV were artificially inoculated to them and natural infection of TYLCV were identified in the field at the same time. The results showed that plants sensitive to TYLCV began to show typical symptoms 20 d after inoculation and the disease fully developed 30 d after inoculation. In the blooming and fruit setting period of plants,most flowers of plants sensitive to TYLCV was wilt and only few fruits were left. The resistant and susceptible plants of individual generation showed regular distribution. Chi-square test showed that the resistance of parent P2 (161-2-3-3-5-2-2-10) to TYLCV was determined only by one dominant gene.

Key words: Tomato yellow leaf curl virus (TYLCV), artificial inoculation, natural disease identification, inheritance of resistance