关键词: 3' RACE, 鸭病毒性肝炎病毒, 序列比对分析, 3' 端非编码区, Poly(A)

Abstract: The authentic 3' end sequences of duck hepatitis virus(DHV)with four strains(ZYM，Z05，Z07 and Z10)were obtained by RACE．The sequence analysis showed that 3' end sequences of the four DHV strains contained 3D region, 3' untranslated region(3' UTR)and a poly(A) tail．The sequencing results showed that the amplified specific sequence was 597nt in length(not including polyA tail)，and the sequences of 3' UTR were all 314 nt in length after the terminator codon TGA．The aligning analysis showed that the 3' end sequences of DHV shared high nucleotide acid identity among the four DHV strains rangingfrom 96．3％ to 100％ while the homology with referenced strain ranged from93．5％ to 99．7％．Phylogenetic analysis of 3'end sequences indicated that these strains shared high nucleotide acid identity．

Key words: 3 'RACE, DHV, sequence analysis, 3' UTR, Poly(A)