Abstract:

To investigate the genetic basis of segregation distortion in indicajaponica hybrid of rice, seven RFP (red fluorescent protein) transgenic lines of japonica rice were crossed with indica rice, and the inheritance of F1 pollens and F2 plants was analyzed using RFP genes integrated at different genomic sites as visual genetic markers. Based on results of the 7F5 transgenic line, a locus near the rice genomic site chr02: 33465170 was responsible for segregation distortion of F1 pollens in the genetic background of the hybrid between japonica rice Nipponbare and indica rice 9311. Pollens from indicajaponica hybrid of the 9A2 transgenic line segregated in 1∶1 (RFP+∶RFP-) ratio while the F2 plants showed segregation distortion, suggesting that a locus linked to the RFP site (chr04: 29587748) controlled segregation distortion of F2 plants by a mechanism irrelevant to the segregation ratio of F1 pollens. The RFP markers facilitated analysis of pollens and progeny plants of indicajaponica hybrids and provide a new means for exploring genetic mechanisms involved in segregation distortion．

Key words: red fluorescent protein (RFP), transgenic rice, indicajaponica hybrid, segregation distortion