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Screening and identification of high\|efficiency cadmium\|removing fungus

  

  1. (College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China)
  • Online:2015-04-25 Published:2015-04-30

Abstract: In order to obtain strains able to efficiently removing cadmium, cadmium\|contaminated soil samples were collected via random sampling method. The first screening test of cadmium\|resistant strains was carried out through pour plate method, and then the selected stains were inoculated in PDA solid medium containing different concentrations of cadmium for further screening. The removal capacities of cadmium\|resistant strain were determined through fermentation tests. It was shown that nine fungi were isolated within the first screening, and were named as PC\|1 to PC\|9 accordingly. In the heavy metal concentration gradients and fermentation tests, strain PC\|8 exhibited the highest Cd\|removing efficiency, as it grew well in the PDA solid medium containing 32 mmol·L-1 Cd2+. Genetic stability tests proved that the cadmium\|resistance trait of PC\|8 was controlled by genetic character. Based on morphological observation and ITS sequence homology comparison, the strain PC\|8 was primarily identified as Curvularia lunata.

Key words: Cd\, resistant strain;fungus;screening;identification;Curvularia lunata