Abstract: A method of solid phase extraction\|high performance liquid chromatography with fluorescent detector （HPLC\|FLD） was developed for the determination of benzo［a］pyrene（B［a］P） in Tegillarca granosa. The analytical procedure included extraction using n\|hexane by ultra\|sonic oscillation， purification using a B［a］P special SPE column and detection by a fluorescent detector. The method exhibited a good linearity over the range of 001-1 μg·mL-1 for benzopyrene with r=0999 2. The average recovery rate was 8147%～8471% and the relative standard deviation was 48%～88% （n=6）. Under optimized conditions， the detection limit of this method was 03 μg·kg-1， and the detection limits of the detector was 10 ng·mL-1. In conclusion， the proposed method exhibited advantages of easy to operate， fast to perform， and high sensitivity and accuracy.

Key words: benzopyrene, Tegillarca granosa, high liquid chromatography\, fluorescent detection, solid phase extraction