Abstract: High temperature is the main reason responsible for the reduction of silkworm production in summer or autumn. In order to observe the proteome change of silkworm haemolymph under long time heat treatment， two\|dimensional electrophoresis and mass spectrometry technologies were employed in the present study. The results showed that a total of 8779%， 673% and 549% protein spots showed additivity， over\|dominance and under\|dominance patterns， respectively. Twenty\|eight differentially expressed protein spots were identified by peptide mass fingerprinting. Among these， 5 spots including heat shock cognate protein （h2）， aminoacylase （h9）， hemolymph juvenile hormone binding protein （h14）， hydroxypyruvate isomerase （h23） and time interval measuring enzyme\|esterase A4 （h24）， were differentially expressed in hybrid and its parents. All of these illustrated that hybrid might directly produce high expression of heat shock cognate protein to resist high temperature stress， and accelerate the step of energy metabolism and growth and development to avoid high temperature damage.

Key words:  heat stress, silkworm, haemolymph, proteome, two\, dimensional electrophoresis, mass spectrometry