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Induction of Renanthera Tom Thumb “Qilin” protocorm\|like bodies using leaves and roots as explants#br#

  

  1. (1 Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China; 2 College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; 3 South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China)
  • Online:2015-07-25 Published:2015-08-03

Abstract: In order to establish the high efficient regeneration system of Renanthera Tom Thumb “Qilin”, its leaves and roots were used as explants to investigate the effects of different growth regulators combinations and different treatments of roots on protocorm\|like bodies(PLBs)formation. The results showed that leaves of Renanthera Tom Thumb “Qilin” cultivated in different combinations of concentrations of 6\|BA and NAA could induce PLBs easily. The highest induction rate reached 6333% and the best culture medium was 1/2 MS+015 g · L-1 citric acid+6 mg · L-1 6\|BA+02 mg · L-1 NAA. While on the other hand, it was difficult for roots to induce PLBs. It was indicated that leaves were more suitable for rapid propagation in vitro as explants.

Key words: Renanthera, explant, protocorm\, like bodies, Ochidaceae