Abstract: The objective of this study was to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. phaseolicola（Psp） and Curtobacterium flaccumfaciens pv. flaccumfaciens（Cff）. Based on the argK gene of Psp in GenBank， the primers PSPF1/PSPR2 were designed. The duplex PCR assay was developed using the combining primers PSPF1/PSPR2 and CffF1/CffR2， which were specific primers for Cff gene. The reaction conditions were optimized and the specificity and sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was confirmed in the artificially inoculated soybean samples imported. Thus， the duplex PCR developed in this study could be used for the simultaneous detection of the two pathogens from imported soybean.

Key words:  Pseudomonas savastanoi pv. phaseolicola, Curtobacterium flaccumfaciens pv. flaccumfaciens, duplex PCR, detection method