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Detection of genetic stability in the regenerated plantlets of Jiangxi Yanshan red bud taro (Colocasia esculenta L. Schott var. cormosus cv. Hongyayu) using RAPD analysis and flow cytometry

  

  1. (College of Life Sciences, Shangrao Normal University, Shangrao 334001, China)
  • Online:2015-12-25 Published:2016-01-05

Abstract:  The RAPD analysis and flow cytometry detection of genetic stability of Jiangxi Yanshan red bud taro regenerated plantlets were studied, which provide a theoretical basis for conservation in vitro of its germplasm resource and factory production of plantlets. The experimental results showed that, the fluorescence intensity value of the first and the second peak of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro had no significant change by flow cytometry detection. DNA of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro were amplified by RAPD using 20 random primers. The amplified fragment size was 200-3 000 bp. The amplification number of 20 primers was 12, 10, 7, 8, 9, 14, 9, 14, 10, 8, 10, 10, 10, 10, 11, 8, 9, 10, 8, 15. 202 site(strip) were obtained, each primer produced 10.1 site, of which polymorphic loci was 160, the average number detected by each primer was 8 and the average percentage of polymorphic loci was 79.21%. Using NTsys210 software, clustering analysis of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro was studied, which were divided into two categories, one category had 1 kind, namely callus regenerated plantlets, another category included 8 kinds of regenerated plantlets and the control group. The results also showed that callus regenerated plantlets had significant differences compared with other 8 kinds of regenerated plantlets and the control group, while there was no difference between the other 8 regeneration plantlets and the control group.

Key words: Jiangxi Yanshan red bud taro(Colocasia esculenta L. Schott var. cormosus cv. Hongyayu), regenerated plantlets, genetic stability, RAPD analysis, flow cytometry detection