Abstract: A new gene， named as SgP5CS， was cloned by homologous cloning method from Suaeda glauca. Bioinformatics analysis showed that the open reading frame of SgP5CS gene was 2 151 bp， encoding 716 amino acids. The isoelectric point and the relative molecular weight of coded protein were 5.71 and 77.4318 u， respectively. Hydrophobic analysis indicated that SgP5CS was a hydrophilic protein and had no transmembrane domain. Nucleotide sequence Blast by ClustalX indicated that the coded protein of SgP5CS shared an identity of 93% with P5CS of Salicornia europaea. Δ1pyrroline5carboxylate synthetase （P5CS） is a key enzyme in the synthesis of proline which is one of the important osmotic regulators in plants. Our research laid a foundation to further study the P5CS function in Suaeda glauca.

Key words: Suaeda glauca, P5CS, proline