PCR-DGGE assisted selection of ammonia degrading bacteria

doi:10.3969/j.issn.1004-1524.2017.02.15

›› 2017, Vol. 29 ›› Issue (2): 286-291.DOI: 10.3969/j.issn.1004-1524.2017.02.15

• Plant Protection • Previous Articles Next Articles

PCR-DGGE assisted selection of ammonia degrading bacteria

ZHANG Zhichao¹, SUN Hong², WU Yifei², WANG Xin², YAO Xiaohong², LIU Yong², TANG Jiangwu^{2, *}, GE Xiangyang^{1, *}

1. State Key Laboratory of Microbiology, Huazhong Agricultural University, Wuhan 430030, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-05-30 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: In the present study, strains able to efficiently degrade ammonia in livestock waste water were screened with traditional enrichment selection method combined with PCR-denaturing gradient gel electrophoresis (DGGE). Twenty-two strains were screened out after domestication under different ammonia concentrations. Among them, 3 strains exhibited relatively higher ammonia degradation abilities, and were named as ZZC-3, ZZC-4 and ZZC-14, respectively, which were identified as Gordonia sp., Denitrobacter sp. and Rhodococcus sp. by 16S rDNA sequence analysis. The PCR-DGGE results confirmed that the Denitrobacter sp. had the highest concentrations in the enriched solution of waste water. Therefore, strain ZZC-4 was selected as the target strain. After incubation for 144 h, the treatment with strain ZZC-4 reached the highest degradation efficiency, which was 90.8% for ammonia nitrogen and 94.7% for COD, respectively.

Key words: PCR-DGGE, ammonia degradation, Denitrobacter sp.

CLC Number:

X703

ZHANG Zhichao, SUN Hong, WU Yifei, WANG Xin, YAO Xiaohong, LIU Yong, TANG Jiangwu, GE Xiangyang. PCR-DGGE assisted selection of ammonia degrading bacteria[J]. , 2017, 29(2): 286-291.

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PCR-DGGE assisted selection of ammonia degrading bacteria

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