Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken

doi:10.3969/j.issn.1004-1524.2017.08.09

›› 2017, Vol. 29 ›› Issue (8): 1290-1296.DOI: 10.3969/j.issn.1004-1524.2017.08.09

• Animal Science • Previous Articles Next Articles

Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken

MU Tong, ZHANG Juan^*, ZHAO Ping, GU Yaling, LIU Liyuan, YANG Yanjun, AN Kelong, WANG You

College of Agriculture, Ningxia University, Yinchuan 750021, China

Received:2017-01-12 Online:2017-08-20 Published:2017-09-06

Abstract

Abstract: By using real-time quantitative PCR (qRT-PCR) expression of ELOVL2 and ELOVL5 in six different tissues (liver, muscle, heart, testis, ovary, abdominal fat) of Ningxia local varieties of Jingyuan chicken were determined, and the statistical analysis was carried out by SAS 8.2 software. The results showed that the expression of ELOVL2 and ELOVL5 genes were observed in six different tissues from Jingyuan chicken. The relative expression of ELOVL2 gene in liver was the highest, 71.52±0.41, significantly higher than that in other tissues (P<0.05), followed by the ovaries, testicles, abdominal fat, heart and muscle. There was a difference between the latter five tissues, but the difference was not significant (P>0.05). The relative expression of ELOVL5 gene in different tissues of Jingyuan chicken was as followed: liver>abdominal fat>ovary>testicle>heart>muscle. The highest expression level in liver was 110.94±0.02, which was significantly higher than that in other tissues (P<0.05), and the expression in abdominal fat was significantly higher than that in ovary, testis, heart and muscle (P<0.05). In addition, there was no significant difference in the expression in heart and muscle (P>0.05). The expression of ELOVL2 and ELOVL5 genes in different tissues was analyzed and compared. The results showed that the expression of two genes in abdominal fat was significantly different (P<0.05), and the expression of ELOVL5 gene in abdominal fat was higher. The research results showed that there were differences in the expression of ELOVL2 and ELOVL5 genes in six tissues from Jingyuan chicken, and liver tissue was the most obvious, which lays the foundation for the development of local technology platform to build local varieties of molecular breeding and genetic evaluation of germplasm resources.

Key words: Jingyuan chicken, ELOVL2, ELOVL5, relative expression

CLC Number:

S831

MU Tong, ZHANG Juan, ZHAO Ping, GU Yaling, LIU Liyuan, YANG Yanjun, AN Kelong, WANG You. Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken[J]. , 2017, 29(8): 1290-1296.

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Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken

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