Cloning and functional characterization of a Na+/H+ antiporter gene from mulberry

doi:10.3969/j.issn.1004-1524.2017.06.03

›› 2017, Vol. 29 ›› Issue (6): 874-881.DOI: 10.3969/j.issn.1004-1524.2017.06.03

• Crop Science • Previous Articles Next Articles

Cloning and functional characterization of a Na⁺/H⁺ antiporter gene from mulberry

LIU Yan, JI Dongfeng, SHEN Guoxin, WEI Jia, LIN Tianbao, ZHU Yan, LYU Zhiqiang^*

Institute of Sericulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-08-23 Online:2017-06-20 Published:2017-09-07

Abstract

Abstract: Salinity is a major factor that adversely affects the growth and yield of plants worldwide. Plants Na⁺/H⁺ antiporters (NHX) play significant role in adaption to salt stress by compartmentalizing excess cytosolic Na⁺ into vacuoles and maintaining ionic equilibrium. Here we cloned an orthologous of NHX1 gene from You1#, a popular mulberry variety used especially in Southwest China. The length of MaNHX cDNA was 2 016 bp with a deduced molecular mass of 59.28 ku and pI 6.67. The consensus amiloride binding motif (⁸²LFFIYLLPPI⁹¹) was observed in the third putative transmembrane domain. Heterologous expression of MaNHX in Arabidopsis enhanced plants salt tolerance, which was verified by retaining higher chlorophyll content, higher antioxidase activity, slower reudction of malondialdehyde (MDA) content in transgenic lines than those in WT plants when confronted with the NaCl stress. Consequently, our work suggested that MaNHX was a candidate salt tolerance determinant gene in future mulberry breeding.

Key words: Na⁺/H⁺ antiporter pump, salinity stress, mulberry, transgenic

CLC Number:

S718.46

LIU Yan, JI Dongfeng, SHEN Guoxin, WEI Jia, LIN Tianbao, ZHU Yan, LYU Zhiqiang. Cloning and functional characterization of a Na⁺/H⁺ antiporter gene from mulberry[J]. , 2017, 29(6): 874-881.

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Cloning and functional characterization of a Na⁺/H⁺ antiporter gene from mulberry

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