Comparison of crude polysaccharides extraction from marine diatom under different methods

doi:10.3969/j.issn.1004-1524.2018.02.15

›› 2018, Vol. 30 ›› Issue (2): 280-289.DOI: 10.3969/j.issn.1004-1524.2018.02.15

• Food Science • Previous Articles Next Articles

Comparison of crude polysaccharides extraction from marine diatom under different methods

CHEN Lihua, WANG Xin^*, CHENG Jiayang

School of Environment and Energy, Peking University Shenzhen Graduate school, Shenzhen 518055, China

Received:2017-04-28 Online:2018-02-20 Published:2018-02-11

Abstract

Abstract: Crude polysaccharide was extracted from marine diatom biomass cultured outdoor by using several methods (water extraction, acid extraction, alkali extraction, ultrasonic assisted extraction and freeze thawing assisted extraction method). The extraction method was optimized by comparing extraction rates, total polysaccharides content, protein content and sulfate groups content of the crude polysaccharides under different methods. The results showed that the lowest extraction rate was found in the case of water extraction, and the highest value was found in the case of concentrated alkaline (4% NaOH) method. The total polysaccharides content of the crude polysaccharides extracted by physical methods (ultrasonic, freeze+thawing combined with heated water method) were relatively higher than that by chemical methods, with the highest value under freeze thawing +90 ℃ method and the lowest value under acid extraction method. However, the sulfate groups content showed the highest value under acid method and the lowest under concentrated alkaline method. Considering the extraction rate of crude polysaccharides and components of crude polysaccharides, the total polysaccharides content in unit diatom biomass was calculated and showed a trend as: concentrated alkaline+freeze thawing>concentrated alkaline+freeze thawing+90 ℃>concentrated alkaline>acid extraction>ultrasonic+freeze thawing +90 ℃>alkali extraction>ultrasonic+freeze thawing>freeze thawing+90 ℃; and the highest sulfate groups content was found when using concentrated alkaline+freeze thawing and the lowest one was alkali extraction. Therefore, it could be concluded from these results that concentrated alkaline+freeze thawing was the most suitable method for crude polysaccharides extraction from marine diatom which could keep the sulfate groups content at a relatively high level. The concentrated alkaline+freeze thawing method was further used for crude polysaccharides extraction from four isolated diatom species. Results suggested that the total polysaccharides content in unit dry mass of Entomoneis sp. was higher than that of the other three diatom species (24%). However, Chaetoceros sp. showed the highest sulfate groups content (5.05%), and Entomoneis sp. showed the lowest (0.51%).

Key words: crude polysaccharide, marine diatom, total polysaccharides, sulfate groups

CLC Number:

S986.5

CHEN Lihua, WANG Xin, CHENG Jiayang. Comparison of crude polysaccharides extraction from marine diatom under different methods[J]. , 2018, 30(2): 280-289.

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Comparison of crude polysaccharides extraction from marine diatom under different methods

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