Abstract: Molecularly imprinted technology (MIT) was used for the synthesis of tetracyclines molecularly imprinting polymers (MIPs), which were used as the filler in the preparation of solid phase extraction column. Then high performance liquid chromatography (HPLC) was applied to determine the tetracyclines residues in milk. The MIPs with specific adsorption ability were synthesized by precipitation polymerization in acetone acetonitrile mixed solution, with doxycycline hydrochloride (DC) as the template molecule, methacrylic acid (MAA) as the function monomers and ethylene glycol dimethyl acrylic ester (EGDMA) as the crosslinking agent. Their ability of adsorbing tetracycline antibiotics was detected by HPLC, and the selected MIPs were used as filler to prepare a solid phase extraction column for the detection of tetracycline antibiotics residues in milk. It was shown that the tetracycline antibiotics presented a good linear correlation within 0.05-10.0 μg·mL^-1 and the recovery rates of tetracyclines were around 79.4%-86.3%, and the relative standard deviations were all less than 3.8%. The detection limits were 0.02 μg·mL^-1 for tetracycline and oxytetracycline, and was 0.05 μg·mL^-1 for chlortetracycline. The method was accurate, sensitive and specific.

Key words: molecular imprinted polymers, tetracycline antibiotics, solid phase extraction, determination method