Genome-wide identification and expression analysis of soybean NIPs

doi:10.3969/j.issn.1004-1524.2018.07.01

›› 2018, Vol. 30 ›› Issue (7): 1101-1109.DOI: 10.3969/j.issn.1004-1524.2018.07.01

• Crop Science • Previous Articles Next Articles

Genome-wide identification and expression analysis of soybean NIPs

WU Wei¹, FENG Zhijuan², XU Shengchun², LIU Na², ZHANG Guwen², HU Qizan², GONG Yaming^{2, *}

1.Zhejiang Provincial Seed Management Station, Hangzhou 310020, China;
2. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-11-06 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: Nodulin 26-like intrinsic protein (NIPs) was one subclass of aquaporins which played vital roles in abiotic stress responses. Based on soybean genome database, a total of 13 GmNIP genes were identified on the genome level. Chromosome location analysis showed that these GmNIPs located on 11 chromosomes. Physical and chemical properties analyses revealed that the amino acid number of GmNIP proteins ranged from 261 to 296, with molecular masses of 27 to 32 ku and isoelectronic points of 6 to 10. Protein multiple alignments showed that all GmNIPs contained the typical regions of aquaporins: six putative trans-membrane domains (TM1 to TM6) and two NPA (Asp-Pro-Ala box) domains. Phylogenetic analysis showed that the classification of GmNIPs from soybean was consistent with Arabidopsis and rice NIPs. Gene structure analysis revealed that all GmNIPs were consisted of five exons and four introns. Promoter region analysis indicated that the upstream promoter region of most GmNIPs had stress and hormone responsive elements. Tissue expression analysis suggested that five GmNIPs had relatively high transcripts. Furthermore, the expression profile of GmNIP1;5 in response to drought stress was analyzed by the real-time PCR.

Key words: soybean, NIP, aquaporin, genome-wide identification, tissue expression, drought stress response

CLC Number:

S643.1

WU Wei, FENG Zhijuan, XU Shengchun, LIU Na, ZHANG Guwen, HU Qizan, GONG Yaming. Genome-wide identification and expression analysis of soybean NIPs[J]. , 2018, 30(7): 1101-1109.

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Genome-wide identification and expression analysis of soybean NIPs

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