Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents

doi:10.3969/j.issn.1004-1524.2018.09.15

›› 2018, Vol. 30 ›› Issue (9): 1548-1554.DOI: 10.3969/j.issn.1004-1524.2018.09.15

• Plant Protection • Previous Articles Next Articles

Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents

ZHONG Yongjun^{1, 2}, HE Xinwei^{2, *}, YU Dayong², JIANG Jingjing², JIANG Jingyong³

1. Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China;
2. School of Life Sciences, Taizhou University, Taizhou 318000, China;
3. Taizhou Academy of Agricultural Sciences, Linhai 317000, China

Received:2017-12-18 Online:2018-09-25 Published:2018-10-15
Contact: 何昕蔚,E-mail: 710166263@qq.com
Supported by:
浙江省自然科学基金青年基金(LQ18D050001); 台州市科技计划(1701ny06,14NY02); 台州市大学生科技创新项目

Abstract

Abstract: To control the bacterial canker disease, a strain of Pseudomonas syringae pv. actinidae (Psa) from main kiwifruit planting orchards in Taizhou was isolated by using streak plate method in this study. Biovar specific primer detection results showed that the Psa strain was biovar 3. Through the comparison of antibacterial rings, 23 fungicides were examined. The results showed that the antibacterial effect of different fungicides on the Psa strain was quite different, and tetracycline, ethylicin and eugenol exhibited a strong antibacterial effect (the diameter of inhibitory ring≥15 mm). In addition, the antibacterial effect of nano zinc oxide (nano-ZnO) on the Psa strain was examined by minimum inhibitory concentration (MIC) and in vitro time-kill experiments. The results showed that the MIC value of nano-ZnO was 50 μg·mL^-1, and the antibacterial potential increased with the concentration of nano-ZnO and the time of action.

Key words: kiwifruit bacterial canker, Pseudomonas syringae pv. actinidae, biovar, drug sensitivity test, nano zinc oxide

CLC Number:

S436.634

ZHONG Yongjun, HE Xinwei, YU Dayong, JIANG Jingjing, JIANG Jingyong. Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents[J]. , 2018, 30(9): 1548-1554.

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Molecular identification of kiwifruit canker pathogen in Taizhou and screening of its control agents

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