›› 2019, Vol. 31 ›› Issue (1): 74-79.DOI: 10.3969/j.issn.1004-1524.2019.01.10

• OAnimal Science • Previous Articles     Next Articles

Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

GUO Liang1,2,3, LI Chundong1,2, XU Weiliang1,2,3, GUO Yuansheng1,2,3   

  1. 1. Xilingol Vocational College, Xilinhot 026000,China;
    2. Xilingol Institute of Bioengineering, Xilinhot 026000,China;
    3.Xilingol Food Testing and Risk Assessment Center, Xilinhot 026000,China
  • Received:2018-03-21 Online:2019-01-25 Published:2019-04-09

Abstract: The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of TaqMan PCR. The results showed that the technique based on the primers and probe was specific for species identification and quantification, and was able to detect 1 pg template. This biotechnological method based on TaqMan PCR was appropriate to identify porcine-derived materials in farm products and foods.

Key words: porcine-derived material, primer, probe, TaqMan PCR

CLC Number: