Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

doi:10.3969/j.issn.1004-1524.2019.01.13

›› 2019, Vol. 31 ›› Issue (1): 98-103.DOI: 10.3969/j.issn.1004-1524.2019.01.13

• Plant Protection • Previous Articles Next Articles

Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

CHEN Xuan¹, ZHANG Tianye¹, YANG Jian², ZHANG Hengmu², CHEN Jianping^2,*

1.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
2.Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-03-25 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: Eukaryotic translation elongation factor (eEFs) is an important multifunctional protein. eEF1β was subunit of the eukaryotic translation elongation factor-1 (eEFs-1) complex and played an important role in protein biosynthesis. The eEF1β gene was obtained by cloning with RT-PCR from wheat and named as TaeEF1β. The amino acid sequences of TaeEF1β were highly conserved and the conserved domain was located in 137-226 aa. The results of qRT-PCR showed that TaeEF1β were up-regulated in the CWMV-infected plants. In this study, the expression of TaeEF1β in the stems, leaves and roots of wheat and different stages of CWMV infection was also detected by qRT-PCR.

Key words: eukaryotic translation elongation factor, Chinese wheat mosaic virus, homology analysis

CLC Number:

S435.12

CHEN Xuan, ZHANG Tianye, YANG Jian, ZHANG Hengmu, CHEN Jianping. Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.[J]. , 2019, 31(1): 98-103.

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Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

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