Study on endophytic bacteria isolated from Eucommia ulmoides with antimicrobial activity against Fusarium graminearum

doi:10.3969/j.issn.1004-1524.2019.05.12

›› 2019, Vol. 31 ›› Issue (5): 766-776.DOI: 10.3969/j.issn.1004-1524.2019.05.12

• Plant Protection • Previous Articles Next Articles

Study on endophytic bacteria isolated from Eucommia ulmoides with antimicrobial activity against Fusarium graminearum

CHEN Xiaojie, WANG Qi, ZHANG Xinyue, DING Ting^*

School of Plant Protection, Anhui Agricultural University, Hefei 230036, China

Received:2018-10-29 Online:2019-05-25 Published:2019-05-23

Abstract

Abstract: This study was carried out to assess the inhibitory activities of endophytic bacteria from Eucommia ulmoides against Fusarium graminearum. The endophytic bacteria from E. ulmoides were isolated and purified, and the antimicrobial activities of endophytic bacteria against F. graminearum were examined using confront antibiotic culture experiment, disease-control pot experiment. Then, the antimicrobial strain was identified at molecular level and its resistance mechanism against F. graminearum was analyzed. The results indicated 104 endophytic bacteria were isolated from the leaves, stems and roots of E. ulmoides, and DZSG09, DZSJ16 and DZSG23 had strong inhibition against F. graminearum, inhibitory rate of DZSG23 on F. graminearum was 69.04%. Moreover, control efficacy of DZGS23 against F. graminearum was 40.89% by pot experiment after inoculation for 10 d. 16S rDNA sequence analysis showed that DZGS23 belonged to Bacillus. DZGS23 could cause the abnormity and tortuosity of mycelial of phytopathogen and so on. Lastly, bacterial suspension and secondary metabolite of DZSG23 had strong teratogenic effect on conidia of F. graminearum conidia. Deformity rates of conidia were 57.70% and 36.67% after treated with suspension of DZSG23 (1×10⁶ CFU·mL^-1) and its secondary metabolites (10 mg·mL^-1) for 36 h, and they could cause abnormality of conidia and made tubes swollen and contort. However, the bacterial suspension and secondary metabolites of DZSG23 weakly affected germination of F. graminearum conidia. The results afforded scientific basis for DZSG23 application.

Key words: Eucommia ulmoides, Bacillus, Fusarium graminearum, antimicrobial activity

CLC Number:

S641.2

CHEN Xiaojie, WANG Qi, ZHANG Xinyue, DING Ting. Study on endophytic bacteria isolated from Eucommia ulmoides with antimicrobial activity against Fusarium graminearum[J]. , 2019, 31(5): 766-776.

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Study on endophytic bacteria isolated from Eucommia ulmoides with antimicrobial activity against Fusarium graminearum

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