Abstract: In this research, effects of different carbon and nitrogen sources, initial pH value, culture temperature and culture time on the activities of cellulase, amylase and xylanase produced by Aspergillar sulphureus SQ-3 were studied. By investigating the influence of different baking temperature on survivability of this fungus and the activities of these enzymes, the results showed that the optimum culture medium for producing enzymes was as follows: 25.7% of corn stalk powder, 5.8% of soybean meal, 8.5% of wheat bran, 28 ℃ as fermentation temperature and initial pH value of 7.0. Under these conditions, higher activities of cellulase, amylase and xylanase were obtained, and they were 453.3 U·g^-1, 3.9×10³ U·g^-1 and 1.48×10⁵ U·g^-1, respectively. The number of viable spores under the baking temperature of 40 ℃ was over 1.1×10⁸ CFU·g^-1 and the activities of xylanase, cellulase and amylase were well preserved. The results indicated that the fermentation conditions listed above were suited to lower-cost and efficient culture for enzymes produced by Aspergillar sulphureus SQ-3.

Key words: Aspergillar sulphureus, cellulase, amylase, xylanase