Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot

doi:10.3969/j.issn.1004-1524.2019.07.10

›› 2019, Vol. 31 ›› Issue (7): 1105-1111.DOI: 10.3969/j.issn.1004-1524.2019.07.10

• Plant Protection • Previous Articles Next Articles

Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot

LIU Zhihui¹, HAO Rongrong¹, XU Yongfeng², YANG Chengde^1,*, ZHANG Junlian³

1. College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China;
2. Zhangye Station of Plant Protection and Quarantine, Zhangye 734000,China;
3. Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070,China;

Received:2018-12-27 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: In this study, Fusarium solani was used as indicative fungi, 30 antagonistic bacteria whose inhibition rates were higher than 70.00% from 120 endophytic bacteria were selected with the method of plate confrontation. Among them, 265ZY1, 265ZY3 and 265XY6 had strong antagonistic effects on F. solani, the inhibition rates reached 72.20%, 73.00% and 72.80%, respectively. Combining them with the ratio of 1∶1∶1, the inhibition rate reached 79.75%. The results showed that 265ZY1, 265ZY3 and 265XY6 had excellent biological functions, which could be developed as biocontrol bacteria. With the observation of morphology and 16S rDNA gene sequence analysis, 265ZY1, 265ZY3 and 265XY6 were identified as Bacillus vallismortis, Bacillus amyloliquefaciens and Bacillus methylotrophicus, respectively.

Key words: Fusarium solani, microbial antagonist, Bacillus vallismortis, Bacillus amyloliquefaciens, Bacillus methylotrophicus, control effect

CLC Number:

S435.32

LIU Zhihui, HAO Rongrong, XU Yongfeng, YANG Chengde, ZHANG Junlian. Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot[J]. , 2019, 31(7): 1105-1111.

References

[1] 王信,程亮,王亚艺,等.青海省马铃薯黑胫病病原菌的鉴定[J].浙江农业学报,2018,30(8):1369-1375.
WANG X, CHENG L, GAO X S, et al.Identification of blackleg pathogen on potato(Solanum tuberosum L.)in Qinghai[J].Acta Agriculturae Zhejiangensis,2018,30(8):1369-1375.(in Chinese with English abstract)
[2] 许苗, 叶文武, 王淑琛, 等. 快速检测马铃薯干腐病病原接骨木镰孢的环介导等温扩增技术[J]. 植物病理学报, 2018, 48(1): 55-60.
XU M, YE W W, WANG S C, et al.Rapid diagnosis of potato dry rot caused by Fusarium sambucinum using a loopmediated isothermal amplification assay[J]. Acta Phytopathologica Sinica, 2018, 48(1): 55-60.(in Chinese with English abstract)
[3] 李金花, 王蒂, 柴兆祥, 等. 甘肃省马铃薯镰刀菌干腐病优势病原的分离鉴定[J]. 植物病理学报, 2011, 41(5): 456-463.
LI J H, WANG D, CHAI Z X, et al.Isolation and identification of the dominant pathogens causing potato Fusarium dry rot in Gansu Province[J]. Acta Phytopathologica Sinica, 2011, 41(5): 456-463.(in Chinese with English abstract)
[4] 魏巍, 赵冬梅, 张岱, 等. 马铃薯干腐病优势病原菌Fusarium sambucinum分子检测技术的建立[J]. 植物保护, 2017, 43(4): 115-119.
WEI W, ZHAO D M, ZHANG D, et al.Molecular identification of dominant pathogen Fusarium sambucinum causing potato dry rot[J]. Plant Protection, 2017, 43(4): 115-119.(in Chinese with English abstract)
[5] 裴慧兰, 王爱军. 马铃薯干腐病发生规律及防治措施[J]. 现代农业科技, 2017(7): 123, 127.
PEI H L, WANG A J. Occurrence and control measures of potato dry rot[J]. Modern Agricultural Science and Technology, 2017(7): 123, 127.(in Chinese with English abstract)
[6] 孙现超, 彭健芳, 张宁, 等. 马铃薯干腐病菌拮抗放线菌JY-22的抑菌作用及菌株鉴定[J]. 植物保护学报, 2013, 40(1): 38-44.
SUN X C, PENG J F, ZHANG N, et al.Inhibitory effect and identification of an antagonistic actinomyces strain JY-22 against Fusarium solani causing potato dry rot[J]. Acta Phytophylacica Sinica, 2013, 40(1): 38-44.(in Chinese with English abstract)
[7] 赵冬梅, 魏巍, 张岱, 等. 马铃薯干腐病室内药剂筛选及防病研究[J]. 湖北农业科学, 2017, 56(17): 3268-3271, 3279.
ZHAO D M, WEI W, ZHANG D, et al.Studies on screening for fungicides and disease prevention of potato dry rot in laboratory[J]. Hubei Agricultural Sciences, 2017, 56(17): 3268-3271, 3279.(in Chinese with English abstract)
[8] GERBORE J, BENHAMOU N, VALLANCE J, et al.Biological control of plant pathogens: advantages and limitations seen through the case study of Pythium oligandrum[J]. Environmental Science and Pollution Research, 2014, 21(7): 4847-4860.
[9] 苏博, 江胡彪, 乔建礼, 等. 玉米纹枯病生防菌DZSY21的抗病机制[J]. 浙江农业学报, 2017, 29(3): 450-459.
SU B, JIANG H B, QIAO J L, et al.Resistance mechanism of bio-control strain DZSY21 against maize sheath blight[J]. Acta Agriculturae Zhejiangensis, 2017, 29(3): 450-459.(in Chinese with English abstract)
[10] 蒋欢, 彭玉梅, 闫玉芳, 等. 榨菜根肿病生防细菌的筛选、鉴定及评价[J]. 植物保护, 2018, 44(2): 104-110.
JIANG H, PENG Y M, YAN Y F, et al.Screening, identification and evaluation of antagonistic bacteria against clubroot of mustard[J]. Plant Protection, 2018, 44(2): 104-110.(in Chinese with English abstract)
[11] 韩长志. 植物病害生防菌的研究现状及发展趋势[J]. 中国森林病虫, 2015, 34(1): 33-37, 25.
HAN C Z.Research status and development trend of microbial antagonists against plant diseases[J]. Forest Pest and Disease, 2015, 34(1): 33-37, 25.(in Chinese with English abstract)
[12] 王瑞霞, 贺运春, 赵廷昌, 等. 马铃薯环腐病生防菌株P1的鉴定、防病效果及促生作用研究[J]. 植物病理学报, 2010, 40(1): 66-73.
WANG R X, HE Y C, ZHAO T C, et al.Identification, disease-preventing role and growth-promoting effect of biocontrol strain P1 to potato bacteria ring rot[J]. Acta Phytopathologica Sinica, 2010, 40(1): 66-73.(in Chinese with English abstract)
[13] 畅涛, 王涵琦, 杨成德, 等. 马铃薯坏疽病Phoma foveata生防菌的筛选及鉴定[J]. 中国生物防治学报, 2014, 30(2): 247-252.
CHANG T, WANG H Q, YANG C D, et al.Screening and identification of antagonist bacteria against Phoma foveata on potato[J]. Chinese Journal of Biological Control, 2014, 30(2): 247-252.(in Chinese with English abstract)
[14] 关小敏, 孟品品, 刘星, 等. 马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究[J]. 干旱地区农业研究, 2015, 33(3): 90-95.
GUAN X M, MENG P P, LIU X, et al.Isolation and identification of antagonistic strains against black scurf of potato and conditions for their growth[J]. Agricultural Research in the Arid Areas, 2015, 33(3): 90-95.(in Chinese with English abstract)
[15] 陈书珍, 陈泰祥, 季绪霞, 等. 甘肃省岷县马铃薯窖藏干腐病鉴定及药剂筛选[J]. 草业科学, 2017, 34(11): 2218-2225.
CHEN S Z, CHEN T X, JI X X, et al.Identification of the pathogen of the potato dry rot disease and screening of fungicides in the Minxian district of Gansu Province[J]. Pratacultural Science, 2017, 34(11): 2218-2225.(in Chinese with English abstract)
[16] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998.
[17] 东秀珠,蔡妙英.常见细菌鉴定手册[M].北京:科学出版社,2001:43-168.
[18] 张春秋, 马光恕, 廉华, 等. 木霉对黄瓜幼苗生理特性及枯萎病防治效果的影响[J]. 植物保护, 2018, 44(5): 238-246.
ZHANG C Q, MA G S, LIAN H, et al.Effect of Trichoderma on physiological characteristics of cucumber seedlings and control effect against Fusarium wilt[J]. Plant Protection, 2018, 44(5): 238-246.(in Chinese)
[19] 颜学明, 刘小芳, 吴尧, 等. 一株马铃薯干腐病病原菌的鉴定与药剂筛选[J]. 湖北农业科学, 2015, 54(22): 5607-5609, 5656.
YAN X M, LIU X F, WU Y, et al.Identification of a strain pathogens isolated from potato dry rot and fungicides screening[J]. Hubei Agricultural Sciences, 2015, 54(22): 5607-5609, 5656.(in Chinese with English abstract)
[20] SCHISLER D A, SLININGER P J, OLSEN N L.Appraisal of selected osmoprotectants and carriers for formulating Gram-negative biocontrol agents active against Fusarium dry rot on potatoes in storage[J]. Biological Control, 2016, 98: 1-10.
[21] SCHISLER D A.Selection and performance of bacterial strains for biologically controlling fusarium dry rot of potatoes incited bygibberella pulicaris[J]. Plant Disease, 1994, 78(3): 251.
[22] SCHISLER D A, SLININGER P J, HANSON L E, et al.Potato cultivar, pathogen isolate and antagonist cultivation medium influence the efficacy and ranking of bacterial antagonists of fusarium dry rot[J]. Biocontrol Science and Technology, 2000, 10(3): 267-279.
[23] 娄义, 郭俏, 彭楚, 等. 3株芽孢杆菌对番茄的促生作用及对番茄根域微生物的影响[J]. 应用生态学报, 2018, 29(1): 260-268.
LOU Y, GUO Q, PENG C, et al.Effects of three Bacillus strains on growth promoting and rhizosphere soil microflora of tomato[J]. Chinese Journal of Applied Ecology, 2018, 29(1): 260-268.(in Chinese with English abstract)
[24] 张猛,王琼,万东光,等.1株死谷芽孢杆菌的分离、鉴定及防治西瓜枯萎病的效果[J].江苏农业科学,2017,45(8):97-100.
ZHANG M, WANG Q, WAN D G, et al.Isolation, identification and control of a Fusarium oxysporum[J]. Jiangsu Agricultural Sciences, 2017, 45(8):97-100.(in Chinese)
[25] 张国漪, 丁传雨, 任丽轩, 等. 菌根真菌和死谷芽孢杆菌生物有机肥对连作棉花黄萎病的协同抑制[J]. 南京农业大学学报, 2012, 35(6): 68-74.
ZHANG G Y, DING C Y, REN L X, et al.The synergetic inhibition effect of arbuscular mycorrhizal fungi cooperated Bacillus vallismortis organic fertilizer on cotton Verticillium wilt[J]. Journal of Nanjing Agricultural University, 2012, 35(6): 68-74.(in Chinese with English abstract)
[26] 魏新燕, 黄媛媛, 黄亚丽, 等. 甲基营养型芽孢杆菌BH21对葡萄灰霉病菌的拮抗作用[J]. 中国农业科学, 2018, 51(5): 883-892.
WEI X Y, HUANG Y Y, HUANG Y L, et al.Antagonism of bacillus methylotrophicus strain BH21 to Botrytis cinerea[J]. Scientia Agricultura Sinica, 2018, 51(5): 883-892.(in Chinese with English abstract)
[27] 周登博, 陈宇丰, 井涛, 等. 抗香蕉枯萎病甲基营养型芽孢杆菌的鉴定及定殖[J]. 中国农学通报, 2017, 33(36): 145-151.
ZHOU D B, CHEN Y F, JING T, et al.Identification and colonization of bacillus methylotrophicus against banana fusarium wilt disease[J]. Chinese Agricultural Science Bulletin, 2017, 33(36): 145-151.(in Chinese with English abstract)
[28] 潘虹余, 金玮鋆, 张晓蒙, 等. 解淀粉芽孢杆菌B15抑菌物质对葡萄灰霉病灰葡萄孢的抑菌机理[J]. 微生物学报, 2018, 58(7): 1245-1254.
PAN H Y, JIN Y J, ZHANG X M, et al.Inhibition of antifungal substances from Bacillus amyloquefaciens B15 against Botrytis cinerea: the agent of “gray mold” of grape[J]. Acta Microbiologica Sinica, 2018, 58(7): 1245-1254.(in Chinese with English abstract)

Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot

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