Cloning and expression analysis of a fibrillin gene in plant

doi:10.3969/j.issn.1004-1524.2019.09.01

›› 2019, Vol. 31 ›› Issue (9): 1399-1404.DOI: 10.3969/j.issn.1004-1524.2019.09.01

• Crop Science • Previous Articles Next Articles

Cloning and expression analysis of a fibrillin gene in plant

JIANG Yaoyao^1,2, LI Jing², CAI Nianjun², CHEN Jianping^3,*, ZHANG Hengmu^2,*

1. College of Forestry and Biotechnology, Zhejiang A&F University, Lin'an 311300, China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Institute of Plant Virology, Ningbo University, Ningbo 315211, China

Received:2019-04-25 Online:2019-09-25 Published:2019-10-11

Abstract

Abstract: Plant fibrillin (FBN), a large family of conserved proteins, is widely distributed in plant kingdom, but its biological properties and functions in Nicotiana benthamiana are still unclear. To analyze its expression and function, an FBN gene (NbFBN) was amplified and cloned from Nicotiana benthamiana by RT-PCR. Phylogenetic tree analysis showed that the gene was closely related to FBN1a and FBN1b in Arabidopsis thaliana. Homology analysis indicated that it was highly homologous to different FBN genes and its C-terminal part was particularly conserved in several kinds of plants. Quantitative analysis showed that NbFBN was highly expressed in leaves and flowers of Nicotiana benthamiana, and that the gene was found to be induced by drought stress and hormone ABA, indicating that the gene might be involved in response to the abiotic stresses.

Key words: plant fibrillin, homology analysis, quantitative PCR (qPCR), abiotic stresses

CLC Number:

JIANG Yaoyao, LI Jing, CAI Nianjun, CHEN Jianping, ZHANG Hengmu. Cloning and expression analysis of a fibrillin gene in plant[J]. , 2019, 31(9): 1399-1404.

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Cloning and expression analysis of a fibrillin gene in plant

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