›› 2019, Vol. 31 ›› Issue (9): 1502-1508.DOI: 10.3969/j.issn.1004-1524.2019.09.14

• Horticultural Science • Previous Articles     Next Articles

Study on preservation and germination of Cymbidium hybridum pollen

ZHANG Xiaoying, FU Qiaojuan*, ZHAO Fukang, LI Chunnan, RUAN Ruoxin   

  1. Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
  • Received:2019-04-25 Online:2019-09-25 Published:2019-10-11

Abstract: To solve the problem of the flowering worst of orchid and provide reference for orchid hybridization breeding. This study have used the Cymbidium hybridum species JOIC and KK560 as test materials to screen the optimal medium for in vitro pollen germination, and detected the in vitro germination rate of Cymbidium hybridum pollen with different storage temperature and storage time to determine the appropriate pollen preservation method. The results showed that the optimum medium for in vitro germination of JOIC pollen was 0.5 MS+30% sucrose, and the optimum medium for in vitro germination of KK560 pollen was 0.01% H3BO3+0.2% CaCl2+30% sucrose. Pollinium should be dried in the shade with silicone for 24 h before preservation and be preserved in -20 ℃ for six months.

Key words: Cymbidium hybridum, pollen preservation, pollen viability, in vitro germination

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