Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species

doi:10.3969/j.issn.1004-1524.2020.01.05

›› 2020, Vol. 32 ›› Issue (1): 35-42.DOI: 10.3969/j.issn.1004-1524.2020.01.05

• Animal Science • Previous Articles Next Articles

Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species

YUAN Donghao¹, YANG Tianyan^1,*, MENG Wei², ZHENG Yao¹, ZHENG Deyu¹

1. Fishery College, Zhejiang Ocean University, Zhoushan 316022, China;
2. Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, Ministry of Agriculture and Rural Affairs, Marine Fishery Research Institute of Zhejiang Province, Zhoushan 316021, China

Received:2019-06-13 Online:2020-01-25 Published:2020-03-11

Abstract

Abstract: Mitochondrial DNA cytochrome b gene sequence fragments of four Sillago species (Sillago aeolus, S. asiatica, S. sihama and S. japonica) were sequenced and analyzed to compare the sequence differences, phylogenetic relationships and taxonomic status among them. The results showed that the average nucleotide composition of the four species was T 29.9%, C 29.2%, A 21.5% and G 19.3%, and the average interspecific genetic distance was between 0.157 and 0.280. The maximum likelihood (ML) phylogenetic tree showed that S. japonica and S. asiatica clustered together first, and then clustered with S. sihama and S. aeolus, respectively. S.aeolus was the latest to divergent from other Sillago species. The genetic divergence time of four Sillago fishes based on the nucleotide divergence rate of Cyt b gene sequence was 785-1400 years ago in the Miocene period.

Key words: Sillago, mitochondrial cytochrome b gene, sequence variance, phylogenetics, divergence time

CLC Number:

S917

YUAN Donghao, YANG Tianyan, MENG Wei, ZHENG Yao, ZHENG Deyu. Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species[J]. , 2020, 32(1): 35-42.

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Sequence variation and molecular phylogeny of mitochondrial Cyt b gene segments from four Sillago species

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