Obtaining and genetic analysis of hybrid progeny between Brassica napus cv. Xiangyou 15 and Brassica campestris ssp. pekinensis

doi:10.3949/j.issn.1004-1524.2021.07.02

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1170-1176.DOI: 10.3949/j.issn.1004-1524.2021.07.02

• Crop Science • Previous Articles Next Articles

Obtaining and genetic analysis of hybrid progeny between Brassica napus cv. Xiangyou 15 and Brassica campestris ssp. pekinensis

CHEN Jipeng(), LIU Xiaolin, LI Shengqiang, LIU Xianjun, HU Yueqing, CHEN Tao

Key Laboratory of Regulation of Crop Growth and Development in Jiangxi Province, College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China

Received:2020-10-01 Online:2021-07-25 Published:2021-08-06

Abstract

Abstract:

In the present study, Brassica napus cv. Xiangyou 15 and B. campestris ssp. pekinensis were used as parents to obtain the interspecific hybrid, and the second generation was gained by self-crossing to obtain rapeseed germplasm with valuable traits. And the meiosis of pollen mother cells (PMCs) were observed via microscope. The results showed that it was easier to obtain hybrid progenies when B. napus was used as female parent, but it was more difficult when B. campestris was used as female parent. The plant type and leaf morphology of progenies from reciprocal crosses were similar to those of B. napus. At the diakinesis, the majority of the chromosomes formed bivalents or univalents, and a few of chromosomes formed multivalents. At metaphase and anaphase, laggard chromosomes and anaphase bridges were observed in PMCs. The stamen did not develop very well and a lot of pollens were not fertile, and the seed setting rate was relatively low in the first generation. The pollen fertility and seed setting rate of the second generation were significantly (P<0.01) higher than those of the first generation. It is expected that the hybrid fertility will be restored well via selfing crossing, which can be used as genetic resources for further genetic improvement of B. napus.

Key words: Brassica napus, Brassica campestris, interspecific hybridization, meiosis, fertility

CLC Number:

S565.4

CHEN Jipeng, LIU Xiaolin, LI Shengqiang, LIU Xianjun, HU Yueqing, CHEN Tao. Obtaining and genetic analysis of hybrid progeny between Brassica napus cv. Xiangyou 15 and Brassica campestris ssp. pekinensis[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1170-1176.

Figures/Tables 4

References 20

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杂交组合 Combination	授粉花蕾数 Flowers pollinated	形成角数 Pods per plant	形成种子数 Seeds per pod	成苗数 Plants number	杂种数 Hybrids number	亲和指数 Affinity index/%
AACC×AA	345	228	380	256	156	45
AA×AACC	275	30	42	31	12	4

杂交组合 Combination	授粉花蕾数 Flowers pollinated	形成角数 Pods per plant	形成种子数 Seeds per pod	成苗数 Plants number	杂种数 Hybrids number	亲和指数 Affinity index/%
AACC×AA	345	228	380	256	156	45
AA×AACC	275	30	42	31	12	4

杂交组合 Combination	株高 Plant height/cm	第一分枝高度 Branching position/cm	一级分枝数 Number of first branches	可育花粉比例 Ratio of fertile pollens/%	每株角果数 Pods per plant	每个角果种子粒数 Seeds per pod
AACC×AA	205.2±26.0	45.2±10.4	13.5±3.7	100.0±0	458.5±84.5	25.6±4.8
AA×AACC	152.9±15.5	20.8±8.5	10.1±3.0	97.1±2.1	202.5±58.2	17.5±4.0
F₁	228.5±25.0	40.8±12.7	16.5±2.0	49.4±5.6	480.8±52.2	7.4±3.8
F₁'	197.2±18.3	38.6±17.2	11.5±2.5	47.8±4.0	352.8±47.5	4.5±2.4
F₂	211.3±17.5	45.2±11.8	15.6±5.4	61.7±7.4	461.4±63.3	10.2±5.5
F₂'	215.5±15.6	40.6±13.4	12.4±4.5	55.9±3.7	361.1±21.9	7.1±2.1

杂交组合 Combination	株高 Plant height/cm	第一分枝高度 Branching position/cm	一级分枝数 Number of first branches	可育花粉比例 Ratio of fertile pollens/%	每株角果数 Pods per plant	每个角果种子粒数 Seeds per pod
AACC×AA	205.2±26.0	45.2±10.4	13.5±3.7	100.0±0	458.5±84.5	25.6±4.8
AA×AACC	152.9±15.5	20.8±8.5	10.1±3.0	97.1±2.1	202.5±58.2	17.5±4.0
F₁	228.5±25.0	40.8±12.7	16.5±2.0	49.4±5.6	480.8±52.2	7.4±3.8
F₁'	197.2±18.3	38.6±17.2	11.5±2.5	47.8±4.0	352.8±47.5	4.5±2.4
F₂	211.3±17.5	45.2±11.8	15.6±5.4	61.7±7.4	461.4±63.3	10.2±5.5
F₂'	215.5±15.6	40.6±13.4	12.4±4.5	55.9±3.7	361.1±21.9	7.1±2.1

杂交组合 Combination	2n	花粉母细胞数 Number of pollen mother cells	各染色体联会形式的数量(比例) Number (proportion) of various associations
杂交组合 Combination	2n	花粉母细胞数 Number of pollen mother cells	9Ⅰ	7Ⅰ	5Ⅰ	1Ⅲ	1Ⅳ
F₁	29	58	27(47%)	18(31%)	11(19%)	2(3%)	0(0)
F₁'	29	60	26(43%)	19(32%)	11(18%)	2(3%)	2(3%)
F₂	25~27	57	24(42%)	17(30%)	12(21%)	3(5%)	1(2%)
F₂'	25~27	63	30(48%)	19(30%)	9(15%)	2(3%)	3(5%)

Obtaining and genetic analysis of hybrid progeny between Brassica napus cv. Xiangyou 15 and Brassica campestris ssp. pekinensis

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