Expression of ERβ in hypothalamic-pituitary-ovarian axis of Ganjia Tibetan sheep in estrus cycle

doi:10.3969/j.issn.1004-1524.2022.01.02

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (1): 10-16.DOI: 10.3969/j.issn.1004-1524.2022.01.02

• Animal Science • Previous Articles Next Articles

Expression of ERβ in hypothalamic-pituitary-ovarian axis of Ganjia Tibetan sheep in estrus cycle

BAO Yingying¹(), CHEN Weigang¹, GE Wenbo², HE Yuqin¹^,^*(), YANG Zhijie¹, ZHOU Kairen², YANG Dapeng², YANG Yawen¹, LIU Lili¹

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-10-17 Online:2022-01-25 Published:2022-02-05
Contact: HE Yuqin

Abstract

Abstract:

To evaluate the expression and distribution of estrogen receptor β (ERβ) in the hypothalamus-pituitary-ovarian (HPO) axis of Ganjia Tibetan sheep in estrum cycle, thirty-two sheep with healthy and non-pregnant Ganjia Tibetan sheep during the estrus cycle were collected from the Tibetan sheep farm. Real-time fluorescent quantitative polymerase, Western blot and immunohistochemical staining were used to detect the expression and distribution of ERβ mRNA and its protein in HPO axis of Ganjia Tibetan sheep at different stages of the estrus cycle. The results showed that ERβ mRNA and its protein were expressed and distributed in hypothalamus, pituitary and ovary during the whole estrus cycle (proestrus, estrus, metestrus and diestrus) of Ganjia Tibetan sheep. ERβ mRNA and its protein were significantly higher in the proestrus as compared with the other three stages (P<0.05) in hypothalamus. It was found that ERβ mRNA and protein in proestrus was significantly higher than the other three stages (P<0.05) in pituitary, and the highest expression was found in ovarian tissue in diestrus and significantly higher than the other three stages(P<0.05). The immunohistochemical results reported that the positive expression of ERβ was mainly distributed in large neuronal cytoplasm and axons. The stronger expression was observed in the eosinophilic cytoplasm in distal and intermediate parts of the pituitary, but weaker expression was found in nucleus. In ovary, ERβ positive reaction was distributed in cytoplasm of growth follicular granulosa cells and nuclei, follicular endometrium cells and luteal cells. The different expression of ERβ in the HPO axis in the estrus cycle of Ganjia Tibetan sheep suggests that ERβ has important significance for reproductive physiological activities such as the estrus cycle of Tibetan sheep.

Key words: Ganga Tibetan sheep, ERβ, hypothalamus-pituitary-ovarian axis, estrus cycle

CLC Number:

S826

BAO Yingying, CHEN Weigang, GE Wenbo, HE Yuqin, YANG Zhijie, ZHOU Kairen, YANG Dapeng, YANG Yawen, LIU Lili. Expression of ERβ in hypothalamic-pituitary-ovarian axis of Ganjia Tibetan sheep in estrus cycle[J]. Acta Agriculturae Zhejiangensis, 2022, 34(1): 10-16.

Figures/Tables 4

Table 1 Information of primer

基因 Gene	引物序列(5'~3') Primer sequences	产物大小 Product size/bp	Tm/℃	用途 Application	登录号 Login ID
ERβ	F: GCTCTGGTCTGGGTGATT R: TGTTCCATGCCCTTGTTA	119	58	目的基因 Target gene	NM 0010009737.1
β-actin	F:CTTCCAGCCTTCTCCTTCCTGG R:GCCAGGGCAGTGATCTCTTT	180	58	内参基因 Reference gene	HM067830.1

Fig.1 Relative expression levels of ERβ mRNA in HPO axis of Ganjia Tibetan sheep during the estrus cycle A, Hypothalamic;B, Pituitary;C, Ovarian. PE, Proestrus; E, Estrus; ME, Metestrus; DE, Diestrus. The different lowercase indicated significant difference (P<0.05). The same as below.

Fig.2 Relative expression levels of ERβ protein in in HPO axis of Ganjia Tibetan sheep during the estrus cycle

Fig.3 The location of positive reaction of ERβ in HPO axis of Ganjia Tibetan sheep during the estrus cycle A, F, K, Proestrus; B, G,L, Estrus; C, H,M, Metestrus; D,I,N, Diestrus; E,J,O, Negative control. GC, Glial cell; NC, Nerve cell; Nu, Neuronuclei; AC, Acidophilic cell; BC, Basophilic cell; CC, Chromophobe cell; PD, Pars distalis; PI, Pars intermedie; FA, Follicular antrum; GV, Germinal vesicle; LC, Luteal cell; TF, Theca folliculi; SG, Stratum granulosum.

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Expression of ERβ in hypothalamic-pituitary-ovarian axis of Ganjia Tibetan sheep in estrus cycle

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