Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

doi:10.3969/j.issn.1004-1524.2022.11.19

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (11): 2504-2511.DOI: 10.3969/j.issn.1004-1524.2022.11.19

• Food Science • Previous Articles Next Articles

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

LIN Yuqing¹^,²(), LU Shengmin², ZHOU Wanyi², XING Jianrong², YANG Ying²^,^*()

1. College of Food Science and Technonlogy, Nanjing Agricultural University, Nanjing 210095, China
2. Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-03-28 Online:2022-11-25 Published:2022-11-29
Contact: YANG Ying

Abstract

Abstract:

To investigate the preliminary structure and probiotic properties of three Dendrobium officinale leaf polysaccharides in the Yandang Mountain area, and provide basis for high-value utilization of D. officinale leaf resources, leaves of D. officinale Yanhu No.1, D. officinale Yanhu No.3 and D. officinale Shenglan No.8 were used as raw materials, and pure polysaccharides were obtained, which were named as DOP1, DOP2 and DOP3. The relative molecular weight of DOP1, DOP2, and DOP3 were 2.56×10⁵, 1.35×10⁵ and 3.26×10⁵, respectively. All of them were composed of arabinose, galactose, glucose and mannose, but with different molar ratios. DOP1 had the tightest organizational structure in aqueous solution, followed by DOP3, and DOP2 was the loosest. The proliferation of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus rhamnosus were promoted by all three polysaccharides, among which DOP3 had the most significant effect. DOP2 and DOP3 significantly inhibited the growth of Escherichia coli and Listeria monocytogenes, while DOP1 only inhibited the growth of L. monocytogenes. The relative abundance of Parabacteroides and Koalabacteria were increased and the relative abundance of conditional pathogenic bacteria such as Klebsiella and Bliophila were decreased under the treatment of three polysaccharides in human colon flora. In addition, DOP3 also significantly inhibited the growth of Morganella and Pseudomonas. All three polysaccharides could promote the production of acetic acid, propionic acid and butyric acid, among which DOP2 and DOP3 had better effects than DOP1. The results showed all of these three polysaccharides had probiotic functions, among which DOP3 had a better comprehensive effect and had the potential to be used as a new probiotic factor.

Key words: Dendrobium officinale leaf, polysaccharide, colonic flora, short-chain fatty acid, probiotic property

CLC Number:

LIN Yuqing, LU Shengmin, ZHOU Wanyi, XING Jianrong, YANG Ying. Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2504-2511.

Figures/Tables 8

References 28

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名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	植物乳杆菌 Lactobacillus plantarum	戊糖乳杆菌 Lactobacillus pentosus	干酪乳杆菌 Lactobacillus casei	鼠李糖乳杆菌 Lactobacillus rhamnosus
Glu(CK)	1.8±0.6 b	12.9±1.2 b	6.4±0.4 b	1.3±0.1 de
DOP1	3.2±0.5 b	7.0±1.2 c	2.3±0.5 d	1.2±0.1 de
DOP2	3.3±0.2 b	4.6±0.8 d	3.5±0.4 d	2.9±0.8 bc
DOP3	2.8.±6.0 b	6.1±1.4 cd	2.7±0.3 d	1.1±0.1 e
Glu+DOP1	2.7±0.6 b	13.0±1.4 b	9.1±1.2 a	2.9±0.5 ab
Glu+DOP2	3.9±0.3 b	15.8±4.7 b	7.4±1.1 b	3.2±0.7 a
Glu+DOP3	14.9±0.4 a	18.7±0.9 a	6.7±0.5 b	2.0±0.1 cd

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

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Figures/Tables 8

References 28

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碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	大肠埃希菌 Escherichia coli	金黄色葡萄球菌 Staphylococcus aureus	单增李斯特菌 Listeria monocytogenes
Glu(CK)	6.5±0.7 a	2.8±0.1 a	10.0±0.9 a
DOP1	5.4±0.6 a	3.0±0.1 a	8.0±0.4 b
DOP2	3.2±0.6 b	3.5±0.5 a	7.0±0.1 b
DOP3	1.6±0.2 c	3.8±0.8 a	6.6±0.8 b

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