啶氧菌酯降解菌的分离鉴定及其降解特性

doi:10.3969/j.issn.1004-1524.2016.09.14

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (9): 1550-1557.DOI: 10.3969/j.issn.1004-1524.2016.09.14

啶氧菌酯降解菌的分离鉴定及其降解特性

吴龙飞¹, 罗香文^{1, 2}, 刘勇^{1, 2}, 李梦晴¹, 杜娇², 张德咏^{1, 2, *}

1.湖南农业大学植物保护学院,湖南长沙 410128;
2.湖南省植物保护研究所, 湖南长沙 410125

收稿日期:2016-04-19 出版日期:2016-09-15 发布日期:2016-11-23
通讯作者: 张德咏,E-mail: dyzhang78@163.com
作者简介:吴龙飞(1990—),男,湖南郴州人,硕士研究生,主要研究方向为农田污染物生物修复。E-mail: wlf814@163.com
基金资助:
公益性行业(农业)科研专项(201203098); 国家自然科学基金(31272067); 现代农业产业技术体系专项资金(CARS-25-B-05)

Isolation and degrading characteristics of picoxystrobin degrading strain

WU Long-fei¹, LUO Xiang-wen^{1, 2}, LIU Yong^{1, 2}, LI Meng-qing¹, DU Jiao², ZHANG De-yong^{1, 2, *}

1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Plant Protection Institute, Changsha 410125, China

Received:2016-04-19 Online:2016-09-15 Published:2016-11-23

摘要/Abstract

摘要： 通过富集培养法筛选分离到1株能以啶氧菌酯为唯一碳源的降解菌株PID-1,采用形态学、生理生化方法,并结合16S rDNA序列系统发育分析,将菌株PID-1初步鉴定为沼泽红假单胞菌(Rhodopseudomonas palustris)。菌株PID-1降解啶氧菌酯的最佳条件为pH 7和35 ℃。在该降解条件下,培养5 d,菌株PID-1对100 mg·L^-1啶氧菌酯的降解率可达83.54%。将啶氧菌酯经PID-1降解后的物质经质谱扫描,通过谱库检索,发现其降解中间产物包括1-(1,5-dimethylhexyl-)-4-methyl-benzene、2,5-bis (1,1-dimethylethyl)-phenol、butyl 2-methyoxyethyl ester、bis (tert-butyldimethylsilyl) ester、1-(3-n-propoxyphenyl)-2-propanone oxime和2-nitro-4-(trifluoromethyl) phenol。

关键词: 啶氧菌酯, 沼泽红假单胞菌, 降解途径

Abstract: The strain PID-1 was screened and isolated by enrichment culture, and was primarily identified as Rhodopseudomonas palustris based on morphology, physiological and biochemical characteristics as well as phylogenetic analysis of 16S rDNA sequences. The optimal degrading conditions of PID-1 were pH 7 and 35 ℃. Under this condition, up to 83.54% of 100 mg·L^-1 picoxystrobin could be degraded by PID-1. The metabolics of picoxystrobin degraed by PID-1 were identified by mass spectrum as 1-(1,5-dimethylhexyl-)-4-methyl-benzene, 2,5-bis (1,1-dimethylethyl)-phenol, butyl 2-methyoxyethyl ester, bis (tert-butyldimethylsilyl) ester, 1-(3-n-propoxyphenyl)-2-propanone oxime and 2-nitro-4-(trifluoromethyl) phenol.

Key words: picoxystrobin, Rhodopseudomonas palustris, degrading pathway

中图分类号:

X592

吴龙飞, 罗香文, 刘勇, 李梦晴, 杜娇, 张德咏. 啶氧菌酯降解菌的分离鉴定及其降解特性[J]. 浙江农业学报, 2016, 28(9): 1550-1557.

WU Long-fei, LUO Xiang-wen, LIU Yong, LI Meng-qing, DU Jiao, ZHANG De-yong. Isolation and degrading characteristics of picoxystrobin degrading strain[J]. , 2016, 28(9): 1550-1557.

参考文献

[1] MALIŃSKI Z T. Studies on in vitro activity of picoxystrobin and its mixtures with other fungicides against Ramulispora herpotrichoides and Ramulispora acuformis [J]. Pestycydy , 2008 (1/2): 75-86.
[2] GAURILČIKIENE I, RONIS A. The effect of strobilurin fungicides on the development of foliar diseases of winter wheat[J]. Agronomy Research , 2006 (Suppl. 1): 177-180.
[3] 阮宏椿, 杨秀娟, 石妞妞,等. 不同杀菌剂对水稻稻曲病菌的室内毒力及田间药效[J]. 福建农业学报, 2013, 28(6):580-583.
RUAN H C, YANG X J, SHI N N, et al. Toxicity and field efficiency of several fungicides against Ustilaginoidea virens [J]. Fujian Journal of Agricultural Sciences , 2013, 28(6): 580-583. (in Chinese with English abstract)
[4] WANG Y, YANG Y, ZHOU Q, et al. Dissipation and residue of picoxystrobin in banana and soil under field conditions[J]. Polish Journal of Environmental Studies , 2014, 23(4):1361-1365.
[5] FAI P B, GRANT A. A rapid resazurin bioassay for assessing the toxicity of fungicides[J]. Chemosphere , 2009, 74(9):1165-1170.
[6] STENRØD M, KLEMSDAL S S, NORLI H R, et al. Effects of picoxystrobin and 4-n-nonylphenol on soil microbial community structure and respiration activity[J]. PloS ONE , 2013, 8(6): e66989.
[7] 谢道燕, 杨振国, 田梅金,等. 7种杀菌剂对桑树及家蚕的安全性评价[J]. 中国农学通报, 2014, 30(22): 310-315.
XIE D Y, YANG Z G ,TIAN M J, et al. Safety evaluation of seven fungicides to Morus alba and Bombyx mor [J]. Chinese Agricultural Science Bulletin , 2014, 30(22):310-315. (in Chinese with English abstract)
[8] MAJONE M, VERDINI R, AULENTA F, et al. In situ, groundwater and sediment bioremediation: barriers and perspectives at European contaminated sites[J]. New Biotechnology , 2015, 32(1):133-146.
[9] HOWELL C C, SEMPLE K T, BENDING G D. Isolation and characterisation of azoxystrobin degrading bacteria from soil[J]. Chemosphere , 2013, 95(1):370-378.
[10] CYCOŃ M, WÓJCIK M, PIOTROWSKA-SEGET Z. Biodegradation of the organophosphorus insecticide diazinon by Serratia , sp. and Pseudomonas , sp. and their use in bioremediation of contaminated soil[J]. Chemosphere , 2009, 76(4):494-501.
[11] HOLT J G. Bergrey&#x0dbc0;&#x0ddb3;s manual of determinative bacteriology[M]. 9th eds. Baltimore: The Willianms & Wilkin s , 1993.
[12] UEYAMA J, KIMATA A, KAMIJIMA M, et al. Urinary excretion of 3-phenoxybenzoic acid in middle-aged and elderly general population of Japan [J] . Environmental Research , 2009, 109(2): 175-180.
[13] TAMURA K, PETERSON D, PETERSON N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology & Evolution , 2011, 28(10): 2731-2739.
[14] 张继伟, 张博, 冯文英,等. 啶氧菌酯原药中主要杂质的制备、鉴定和定量分析[J]. 世界农药, 2015 (4):50-54.
ZHANG J W, ZHANG B, FENG W Y, et al. Synthesis, identification and quantitative analysis of the main impurity of picoxystrobin[J]. World Pesticides , 2015 (4): 50-54. (in Chinese with English abstract)
[15] ZHANG S, YIN L, LIU Y, et al. Cometabolic biotransformation of fenpropathrin by Clostridium species strain ZP3[J]. Biodegradation , 2011, 22(5):869-875.
[16] 张德咏, 谭新球, 罗香文,等. 一株能降解有机磷农药甲胺磷的光合细菌HP-1的分离及生物学特性的研究[J]. 生命科学研究, 2005, 9(3):247-253.
ZHANG D Y, TAN X Q, LUO X W, et al. Isolation of photosynthetic bacteria HP-1 with degradation of organic-phosphorus insecticides and studies on its biodegradation ability and capacity of increasing growth[J]. Life Science Research , 2005, 9(3): 247-253. (in Chinese with English abstract)
[17] 李乐, 李兰生, 孙涛,等. 固定化光合细菌降解氧化乐果[J]. 农业环境科学学报, 2006, 25(增刊): 721-724.
LI L, LI L S, SUN T, et al. Degradation of omethoate by immobilized photosynthetic bacteria[J]. Journal of Agro-Environment Science , 2006, 25(Suppl.): 721-724. (in Chinese with English abstract)
[18] ZHANG S B, ZHANG D Y, YONG L, et al. Degradation characteristics and pathway of fenpropathrin by Rhodopseudomonas sp. strain PSB07-6.[J]. Fresenius Environmental Bulletin , 2009, 18(11):2060-2065.
[19] YIN L B, LIU Y, ZHANG D Y, et al. Isolation and characterization of Rhodopseudomonas sp. S9-1 capable of degrading pyrazosulfuron-ethyl[J]. Advanced Materials Research , 2011, 356-360: 1152-1163.
[20] 揭晶, 赵越. 光合细菌应用的研究进展[J]. 广东药学院学报, 2006, 22(1):113-114.
JIE J, ZHAO Y. Research progress on application of photosynthetic bacteria[J]. Journal of Guangdong College of Pharmacy , 2006, 22(1): 113-114. (in Chinese)
[21] WOL/EJKO E, L/OZOWICKA B, KACZYŃSKI P, et al. The influence of effective microorganisms (EM) and yeast on the degradation of strobilurins and carboxamides in leafy vegetables monitored by LC-MS/MS and health risk assessment [J]. Environmental Monitoring and Assessment , 2016, 188: 64.
[22] CLINTON B, WARDEN A C, HABOURY S, et al. Bacterial degradation of strobilurin fungicides: a role for a promiscuous methyl esterase activity of the subtilisin proteases?[J]. Biocatalysis & Biotransformation , 2011, 29(4):119-129.

啶氧菌酯降解菌的分离鉴定及其降解特性

Isolation and degrading characteristics of picoxystrobin degrading strain

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