不同地理种群稻纵卷叶螟的解毒酶活性及对药剂的敏感性

doi:10.3969/j.issn.1004-1524.2017.01.11

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 73-80.DOI: 10.3969/j.issn.1004-1524.2017.01.11

不同地理种群稻纵卷叶螟的解毒酶活性及对药剂的敏感性

王彩云^{1, 2}, 杨亚军², 徐红星², 郑许松², 田俊策², 鲁艳辉², 吕仲贤^{2, *}

1.浙江师范大学化学与生命科学学院,浙江金华 321004;
2.浙江省农业科学院植物保护与微生物研究所/浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地,浙江杭州 310021;

收稿日期:2016-05-06 出版日期:2017-01-15 发布日期:2017-02-23
通讯作者: 吕仲贤,E-mail:luzxmh2004@aliyun.com
作者简介:王彩云(1991—),女,湖北随州人,硕士研究生,主要从事害虫生物防治研究。E-mail:1529899731@qq.com
基金资助:
国家水稻产业技术体系(CARS-01-17); 国家自然科学基金项目(31501669)

Detoxified enzymatic activities and insecticidal susceptibility of Cnaphalocrocis medinalis from different geographic populations

WANG Caiyun^{1, 2}, YANG Yajun², XU Hongxing², ZHENG Xusong², TIAN Junce², LU Yanhui², LYU Zhongxian^{2, *}

1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-05-06 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 解毒酶活性及对药剂的敏感性是害虫种群重要的特性,明确稻纵卷叶螟不同种群之间的差异性有利于稻纵卷叶螟防控措施的制定。试验分别测定了广西合浦和南宁、湖南长沙和浙江杭州4个地理种群稻纵卷叶螟幼虫解毒酶活性和其对不同药剂(毒死蜱、氯虫苯甲酰胺、甲维盐、多杀菌素)的敏感性,探讨了不同地理种群间解毒酶活性及药剂敏感性的差异。结果表明,稻纵卷叶螟4个不同地理种群间酯酶、谷胱甘肽转移酶和多功能氧化酶具有种群差异性。长沙种群具有较高的酶活性,而合浦种群酯酶活性最低,南宁种群多功能氧化酶活性最低,合浦、南宁和杭州种群具有相似的谷胱甘肽转移酶活性。稻纵卷叶螟4个不同地理种群对不同药剂的敏感性水平有一定的差异,对同种药剂的敏感性差异在1~4倍之间。在所检测的几种药剂中,南宁和杭州种群对毒死蜱的敏感性显著高于合浦和长沙种群;南宁种群对氯虫苯甲酰胺的敏感性显著低于其他种群;合浦种群对甲维盐的敏感性最高;长沙种群对多杀菌素的敏感性最低;浙江杭州种群对多杀菌素敏感性最高。稻纵卷叶螟幼虫解毒酶活性和其对不同药剂的敏感性具有地理种群差异性,应根据不同地理种群的相关特性,因地制宜地制定相关防控方案。

关键词: 稻纵卷叶螟, 种群差异性, 解毒酶, 敏感性, 杀虫剂

Abstract: Detoxified enzymatic activity and insecticidal susceptibilities are important characteristics of the insect populations. Understanding these characteristics of Cnaphalocrocis medinalis from different populations will promote the formulation of tactics on C. medinalis control. Detoxified enzymatic activity and insecticidal susceptibilities of Cnaphalocrocis medinalis from different geographic populations, Hepu (HP) and Nanning (NN) populations from Guangxi, Changsha (CS) population from Hunan and Hangzhou (HZ) population from Zhejiang were determined to clarify the population differences of C. medinalis, and the differences among four populations were discussed in this study. Results showed that activities of esterase, multi-function oxidase and glutathione S-transferase (GST) varied among C. medinalis larvae from 4 geographic populations. CS population had the highest enzymatic activities, while HP population had a lowest esterase activity, NN population had a lowest multi-function oxidase activity and HP, NN, and HZ population had similar GST activity. Bioassay data showed that the differences were observed in the susceptibilities of 4 C. medinalis populations against insecticides, and the susceptibility of C. medinalis against a same insecticide differed with 1-4 folds among 4 populations. Among the four detected insecticides (chlorpyrifos, chlorantraniliprole, emamectin benzoate and spinosad), NN and HZ populations had higher susceptibility against chlorpyrifos than HP and CS populations, NN population had the lowest susceptibility against chlorantraniliprole, HP population had the highest susceptibility against emamectin benzoate, CS population had the lowest susceptibility against spinosad. Results indicated that C. medinalis had the geographic differences on the detoxified enzymatic activity and insecticidal susceptibilities, the differences of geographic populations would be considered into the formulation of the tactics on the C. medinalis control.

Key words: Cnaphalocrocis medinalis, difference on population, detoxified enzyme, susceptibility, insecticide

中图分类号:

S435.112⁺.1

王彩云, 杨亚军, 徐红星, 郑许松, 田俊策, 鲁艳辉, 吕仲贤. 不同地理种群稻纵卷叶螟的解毒酶活性及对药剂的敏感性[J]. 浙江农业学报, 2017, 29(1): 73-80.

WANG Caiyun, YANG Yajun, XU Hongxing, ZHENG Xusong, TIAN Junce, LU Yanhui, LYU Zhongxian. Detoxified enzymatic activities and insecticidal susceptibility of Cnaphalocrocis medinalis from different geographic populations[J]. , 2017, 29(1): 73-80.

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不同地理种群稻纵卷叶螟的解毒酶活性及对药剂的敏感性

Detoxified enzymatic activities and insecticidal susceptibility of Cnaphalocrocis medinalis from different geographic populations

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