浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析

doi:10.3969/j.issn.1004-1524.2017.06.05

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 888-893.DOI: 10.3969/j.issn.1004-1524.2017.06.05

浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析

杨华^1,2, 何祥祥^1,2,3, 肖英平^1,2, 钱鸣蓉^1,2, 张巧艳¹, 唐标^1,2,*

1.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
2.浙江省植物有害生物防治省部共建国家重点实验室培育基地,浙江杭州310021;
3.西北农林科技大学食品科学与工程学院,陕西杨凌 712100

收稿日期:2017-05-12 出版日期:2017-06-20 发布日期:2017-09-07
通讯作者: 唐标,E-mail: tb_411@163.com
作者简介:杨华(1972—),男,浙江绍兴人,硕士,高级畜牧师,研究方向为畜产品质量安全。E-mail: yanghua806@hotmail.com
基金资助:
浙江省农业科学院青年人才培养项目(2017R19R08E01); 浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地(2011DS700124-ZZ1703); 浙江省重点研发项目(2015C02041)

Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province

YANG Hua^1,2, HE Xiangxiang^1,2,3, XIAO Yingping^1,2, QIAN Mingrong^1,2, ZHANG Qiaoyan¹, TANG Biao^1,2,*

1. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

Received:2017-05-12 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 大肠埃希菌是食品中普遍污染的条件致病菌,其耐药性非常严重,耐药基因具有极大的传播风险。为了解浙江省地区禽源大肠埃希菌耐药性特点及分布规律,试验从浙江省3个地区市售冷鲜鸡中分离了59株大肠埃希菌并测定了耐药谱,发现有24种耐药谱,显示了分离株耐药类型的多样性。同时对分离株进行了Multilocus sequence typing (MLST)分子分型,获得38个已知ST型,并发现2个新的ST型。在此基础上,针对7个看家基因进行了系统发育分析,分离株显示了一定的地区差异性。该试验结果可为浙江省禽源大肠埃希菌的耐药性评价和溯源分析提供参考依据。

关键词: 大肠埃希菌, 细菌耐药性, 多位点序列分型(MLST), 系统发育树

Abstract: Escherichia coli is a common opportunistic pathogen in food. Its drug resistance is severe in which the related resistance genes can easily spread. In this study, 59 strains of E. coli from refrigerated chicken in three regions of Zhejiang Province were isolated. Using drug resistant spectrum determination, 24 types of drug resistant spectrum were found, which showed that the drug resistant types of E. coli isolates were multifarious. Meanwhile, all the E. coli isolates were analyzed with MLST genotyping. The results showed that a total of 38 ST types were obtained, and two new ST types were found as well. On this basis, the phylogenetic analysis of 7 housekeeping genes showed a certain degree of the regional differences. This research results can provide scientific reference for the evaluation of drug resistance and traceability analysis of avian Escherichia coli in Zhejiang province, and a wider region in China.

Key words: Escherichia coli, bacterial drug resistance, multilocus sequence typing (MLST), phylogenetic tree

中图分类号:

S852.61

杨华, 何祥祥, 肖英平, 钱鸣蓉, 张巧艳, 唐标. 浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析[J]. 浙江农业学报, 2017, 29(6): 888-893.

YANG Hua, HE Xiangxiang, XIAO Yingping, QIAN Mingrong, ZHANG Qiaoyan, TANG Biao. Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province[J]. , 2017, 29(6): 888-893.

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浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析

Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province

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