11种荆芥种质资源ISSR分析

doi:10.3969/j.issn.1004-1524.2018.04.13

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 614-621.DOI: 10.3969/j.issn.1004-1524.2018.04.13

11种荆芥种质资源ISSR分析

王向东¹, 马艳芝^{2, *}, 客绍英²

1.唐山市农业科学研究院,河北唐山 063001;
2.唐山师范学院生命科学系,河北唐山 063000

收稿日期:2017-08-04 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 马艳芝,E-mail:mayanzhiwxd@163.com
作者简介:王向东(1976—),男,河北承德人,博士,助理研究员,从事作物遗传与育种研究。E-mail:cauwang@qq.com
基金资助:
河北省教育厅项目(ZC2016004); 唐山市科技局项目(15130266a); 唐山师范学院团队支撑重点项目(2017B05)

Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR

WANG Xiangdong¹, MA Yanzhi^{2, *}, KE Shaoying²

1. Tangshan Academy of Agricultural Science, Tangshan 063001, China;
2. Department of Life Sciences, Tangshan Normal University, Tangshan 063000, China

Received:2017-08-04 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 利用ISSR分子标记技术对不同地区的11种荆芥种质资源进行分析,以此来探讨我国荆芥种质资源的遗传多样性。以11种荆芥幼苗为材料,CTAB法提取其基因组DNA;采用正交试验优化ISSR技术体系影响因素;利用100条ISSR引物进行ISSR扩增,最后计算供试材料之间的遗传距离并进行聚类分析。结果表明,最优ISSR-PCR体系,在25 μL体系中,模板DNA 50 ng,引物0.75 μmol·L^-1,Taq DNA聚合酶1 U;100条引物中55条引物可扩增出结果,共扩增出458条条带,平均每个引物扩增出8.3条,其中246条为多态性带,多态性比率为53.71%;11个荆芥种质资源的遗传距离在0.208 3~0.709 1;在遗传距离为0.50处,可将供试的11种荆芥材料分为2大类;在遗传距离为0.40处,可将供试的11种荆芥材料分为5大类,研究结果表明我国荆芥种质资源遗传多样性较低。

关键词: 荆芥, 种质资源, ISSR, 遗传多样性

Abstract: The aim of the study was to analyze the genetic diversity of 11 Schizonepeta tenuifolia Briq.using ISSR, which would investigate the genetic diversity of Schizonepeta tenuifolia Briq. germplasm resources in China. With 11 kinds of Schizonepeta tenuifolia Briq. seedlings as materials, genomic DNA was extracted using the CTAB method. An orthogonal design was used to optimize the ISSR-PCR amplification system. 100 ISSR primers were used in the ISSR amplification. At last it counted the genetic distance between the tested materials and finally the cluster analysis was done. The results showed that a suitable ISSR reaction system was constructed with 25 μL reaction system containing 50 ng DNA, 0.75 μmol·L^-1 primer and 1 U Taq DNA polymerase. There were 55 primers amplified from the 100 primers, 458 bands were amplified, 8.3 bands were amplified from each primer, of which 246 bands were polymorphic, and the polymorphic rate was 53.71%. The genetic distances of 11 Schizonepeta tenuifolia Briq. germplasm resources were between 0.2083-0.7091. When the genetic distance was 0.50, 11 kinds of materials could be divided into 2 categories. When the genetic distance was 0.40, 11 kinds of materials could be divided into 5 categories. In conclusion, the genetic diversity of germplasm resources of Schizonepeta tenuifolia Briq. was lower in China.

Key words: Schizonepeta tenuifolia Briq., germplasm resources, ISSR, genetic diversity

中图分类号:

Q943

王向东, 马艳芝, 客绍英. 11种荆芥种质资源ISSR分析[J]. 浙江农业学报, 2018, 30(4): 614-621.

WANG Xiangdong, MA Yanzhi, KE Shaoying. Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR[J]. , 2018, 30(4): 614-621.

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11种荆芥种质资源ISSR分析

Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR

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