一株乳酸乳球菌对棒曲霉素的去除作用

doi:10.3969/j.issn.1004-1524.2018.07.18

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1237-1243.DOI: 10.3969/j.issn.1004-1524.2018.07.18

一株乳酸乳球菌对棒曲霉素的去除作用

朱瑞瑜^{1, 2}, 黄聪辉¹, 朱燕瑾¹, 尤玉如^{1, 2}, 李玲^{1, 3}, 孙律¹

1.浙江科技学院生物与化学工程学院,浙江杭州 310023;
2.浙江省农产品化学与生物加工技术重点实验室,浙江杭州 310023;
3.浙江省农业生物资源生化制造协同创新中心,浙江杭州 310023

收稿日期:2018-02-20 出版日期:2018-07-20 发布日期:2018-08-02
作者简介:朱瑞瑜(1986—),女,浙江宁波人,博士,讲师,主要从事食品安全方面的研究。E-mail: zhuruiyu7@126.com
基金资助:
浙江省自然科学基金(LQ16C200003)

Patulin reduction by Lactococcus lactis strain

ZHU Ruiyu^{1, 2}, HUANG Conghui¹, ZHU Yanjin¹, YOU Yuru^{1, 2}, LI Ling^{1, 3}, SUN Lü¹

1. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China;
2. Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Products, Hangzhou 310023, China;
3. Zhejiang Collaborative Innovation Center of Chemical and Biological Manufacturing for Agricultural Biological Resources, Hangzhou 310023, China

Received:2018-02-20 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 以筛选得到的可有效去除棒曲霉素的食品生产用菌株乳酸乳球菌MG1363为实验材料,通过改变环境温度、pH对其降解特性进行研究,并通过灭活菌体、提取粗酶液等手段,对乳酸乳球菌MG1363降解棒曲霉素的机理进行初步探究。结果表明,温度、pH对乳酸菌去除棒曲霉素活力的影响显著(P<0.05),去除棒曲霉素的较佳反应环境为30 ℃、pH值5.5~6.0。乳酸乳球菌MG1363既能物理吸附棒曲霉素,也能通过酶解作用降解棒曲霉素,且发挥降解活性的酶为胞内酶。乳酸乳球菌MG1363酶解棒曲霉素为其自身固有的特性,但棒曲霉素可诱导乳酸乳球菌的降解活性。

关键词: 乳酸乳球菌, 棒曲霉素, 机理

Abstract: In the present study, a strain of food-grade microorganism Lactococcus lactis MG1363 was screened for its ability of patulin degradation. The effects of environmental temperature and pH value on patulin degradation by L. lactis MG1363 were assessed, and the mechanisms of patulin degradation were also studied by bacterial cells inactivation and crude enzyme extraction. The results indicated that temperature and pH influenced patulin degradation significantly (P<0.05), and the optimal condition was 30 ℃, pH value 5.5-6.0. The mechanisms of patulin degradation by L. lactis MG1363 involved both physical adsorption and enzymatic degradation, and the degradation relevant enzymes were located inside the bacterial cells. The property of patulin-degrading by L. lactis MG1363 was constitutive, nevertheless, the enzymatic activity of patulin degradation was enhanced by low amount of patulin.

Key words: Lactococcus lactis, patulin, mechanism

中图分类号:

S182
TS201.3

朱瑞瑜, 黄聪辉, 朱燕瑾, 尤玉如, 李玲, 孙律. 一株乳酸乳球菌对棒曲霉素的去除作用[J]. 浙江农业学报, 2018, 30(7): 1237-1243.

ZHU Ruiyu, HUANG Conghui, ZHU Yanjin, YOU Yuru, LI Ling, SUN Lü. Patulin reduction by Lactococcus lactis strain[J]. , 2018, 30(7): 1237-1243.

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一株乳酸乳球菌对棒曲霉素的去除作用

Patulin reduction by Lactococcus lactis strain

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