基于线粒体COⅠ基因序列的吸血蠓近似种鉴定

doi:10.3969/j.issn.1004-1524.2018.11.04

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (11): 1825-1831.DOI: 10.3969/j.issn.1004-1524.2018.11.04

基于线粒体COⅠ基因序列的吸血蠓近似种鉴定

岑常活, 徐宗意, 韩晓静, 常琼琼, 段琛, 侯晓晖^*

遵义医学院,贵州遵义 563000

收稿日期:2018-03-05 出版日期:2018-11-25 发布日期:2018-12-21
通讯作者: 侯晓晖,E-mail: hxh19801122@163.com
作者简介:岑常活(1994—),男,湖南永州人,硕士研究生,研究方向为蠓科昆虫分子鉴定研究。E-mail: 2438510534@qq.com
基金资助:
国家自然科学基金(81802040);遵义市科技创新人才团队培养项目(遵市科合〔2015〕40号);省级和校级大学生创新创业训练计划(201713653004,遵医科院20162303)

Molecular identification of genus Culicoides (Diptera: Ceratopogonidae) based on mtDNA CO Ⅰ gene

CEN Changhuo, XU Zongyi, HAN Xiaojing, CHANG Qiongqiong, DUAN Chen, HOU Xiaohui^*

Zunyi Medical University, Zunyi 563000, China

Received:2018-03-05 Online:2018-11-25 Published:2018-12-21

摘要/Abstract

摘要： 为了快速、准确地鉴别吸血蠓近似种,弥补传统形态学鉴定方法在种类鉴定中存在的操作繁琐、难度大等不足,提出了基于线粒体CO Ⅰ基因序列的吸血蠓近似种鉴定方法。以二囊亚属中的黑色库蠓(Culicoides pelius)、无害库蠓(Culicoides innoxius)、白带库蠓(Culicoides albifascia)、不显库蠓(Culicoides obsoletus)、东方库蠓(Culicoides orientalis)、苏格兰库蠓(Culicoides scoticus)和琉球库蠓(Culicoides actoni)等7种库蠓近似种的部分线粒体CO Ⅰ基因序列作为分析对象,基于Kimura 2-parameter模型分析遗传距离,同时应用MEGA 6.0软件以荒川库蠓(Culicoides arakawai)为外群构建系统发育树(邻接法和最大似然法)。经过Clustal X序列比对及人工校对、编辑后, 7种吸血蠓CO Ⅰ基因长度为494 bp,遗传距离在种内和种间具显著差异(P<0.05),系统发育树中不同库蠓种类各自构成单系(群),同种类不同地理种群聚为一支。本研究初步证实了线粒体CO Ⅰ基因序列可用于吸血蠓近似种的分子鉴定。

关键词: 蠓科, 吸血蠓, 二囊亚属, 近似种, CO I基因

Abstract: In order to make up for the deficiency of complex operation and big difficulty effectively in the traditional morphological species identification of sibling species of blood-sucking midges and differentiate these rapidly and accurately, molecular identification of seven closely related species of the genus Culicoides, C. pelius, C. innoxius, C. albifascia, C. obsoletus, C. orientalis, C. scoticus and C. actoni were studied based on mitochondrial cytochrome c oxidase subunit I gene (CO Ⅰ). Approximately 493 bp of CO Ⅰ gene were sequenced for those 7 species. Estimate genetic distances of CO Ⅰ gene, and statistical analysis showed that intraspecific genetic distances were different from that of interspecies significantly (P<0.05). The phylogenetic relationship within Culicoides indicates that the topologies of all the phylogenetic trees were quite similar. Distinguished between different species of Culicoides clearly, no cross between different species. The same species gathered closely with each other. These results were consistent with those of morphological taxonomy.

Key words: Ceratopogonidae, biting midges, subgenus Avaritia, sibling species, CO I gene

中图分类号:

S855
R384.5

岑常活, 徐宗意, 韩晓静, 常琼琼, 段琛, 侯晓晖. 基于线粒体COⅠ基因序列的吸血蠓近似种鉴定[J]. 浙江农业学报, 2018, 30(11): 1825-1831.

CEN Changhuo, XU Zongyi, HAN Xiaojing, CHANG Qiongqiong, DUAN Chen, HOU Xiaohui. Molecular identification of genus Culicoides (Diptera: Ceratopogonidae) based on mtDNA CO Ⅰ gene[J]. , 2018, 30(11): 1825-1831.

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基于线粒体COⅠ基因序列的吸血蠓近似种鉴定

Molecular identification of genus Culicoides (Diptera: Ceratopogonidae) based on mtDNA CO Ⅰ gene

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