浙江农业学报 ›› 2019, Vol. 31 ›› Issue (5): 704-708.DOI: 10.3969/j.issn.1004-1524.2019.05.04

• 动物科学 • 上一篇    下一篇

白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析

徐小钦1,2,3, 杜雪1, 李国勤1,4, 张龙2, 陶争荣1, 卢立志1,4,*   

  1. 1.浙江省农业科学院 畜牧兽医研究所,浙江 杭州310021;
    2.西华师范大学 生态研究院,四川 南充637000;
    3.南京农业大学 动物科技学院,江苏 南京 210095;
    4.农业农村部农产品信息溯源重点实验室,浙江 杭州 310021
  • 收稿日期:2018-09-28 出版日期:2019-05-25 发布日期:2019-05-23
  • 通讯作者: *卢立志,E-mail: lulizhibox@163.com
  • 作者简介:徐小钦(1988—),女,四川南充人,博士,研究方向为动物遗传育种。E-mail: xuxiaoqin21@126.com
  • 基金资助:
    浙江省农业(畜禽)新品种选育重大科技专项(2016C02054-16);浙江省农业科学院地方科技合作项目(WZ2017002)

Molecular cloning and bioinformatics analysis of white king pigeon (Columba livia) ovalbumin-related protein Y gene

XU Xiaoqin1,2,3, DU Xue1, LI Guoqin1,4, ZHANG Long2, TAO Zhengrong1, LU Lizhi1,4,*   

  1. 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Institute of Ecology, China West Normal University, Nanchong 637000, China;
    3. College of Animal Sciences & Technology, Nanjing Agricultural University, Nanjing 210095, China;;
    4.Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou 310021,China
  • Received:2018-09-28 Online:2019-05-25 Published:2019-05-23

摘要: 旨在获得白羽王鸽(Columba livia)卵清蛋白关联蛋白Y基因(OVALY)的cDNA序列,并进行生物信息分析。研究结果显示:OVALY基因cDNA全长为2 068 bp,其中5'非编码区序列180 bp,3'非编码区序列720 bp,开放阅读框1 164 bp,编码的蛋白分子量约44.19 ku,含氨基酸残基388个(GenBank∶KX230792);与NCBI数据库中朱鹮(Nipponianippon)OVALY基因序列同源性最高(81%),与白尾鹰(Haliaeetusalbicilla)和沙鸡(Pteroclesgutturalis)OVALY基因序列的同源性分别为80%和79%。经潜在糖基化位点和磷酸化位点预测分析发现,OVALY蛋白结构中潜在糖基化位点和磷酸化位点分别为4个和22个;经CDD(conserved domain database)数据库分析发现,OVALY蛋白具有丝氨酸蛋白酶抑制剂家族的反应中心区域,属于丝氨酸蛋白酶抑制剂超家族。

关键词: 白羽王鸽, 卵清蛋白关联蛋白Y(OVALY), RACE

Abstract: The experiment was conducted to clone the whole cDNA of OVALY gene in Columba liviaand analyze the bioinformatic information of the cDNA sequence.The nucleotide sequence of a full-length 2 068 bp cDNA for OVALY was obtained, containing 5'UTR (180 bp), CDS (1 164 bp) and 3'UTR (720 bp), and coding 388 amino acids (44.19 ku, GenBank accession number: KX230792). The OVALY cDNA sequence of white king pigeon was most similar to Nipponianippon, and the homology was 81%. This sequence was also similar to Haliaeetusalbicilla and Pteroclesgutturalis, and the homologies were 80% and 79%, respectively. 4 N-glycosylated sites and 22 phosphorylation sites were detected in these amino acids. Conserved domain analysis of OVALY protein showed this protein had a conserved domain for serpin superfamily.

Key words: Columba livia, OVALY, RACE

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