饲料磷真消化率不同的山羊空肠菌群结构差异研究

doi:10.3969/j.issn.1004-1524.2019.07.04

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (7): 1057-1065.DOI: 10.3969/j.issn.1004-1524.2019.07.04

饲料磷真消化率不同的山羊空肠菌群结构差异研究

金磊, 王立志^*, 王之盛, 薛白, 彭全辉

四川农业大学动物营养研究所,四川成都 611130

收稿日期:2018-11-05 出版日期:2019-07-25 发布日期:2019-08-07
通讯作者: *王立志,E-mail: wanglizhi08@aliyun.com
作者简介:金磊(1992—),男,陕西西安人,硕士研究生,研究方向为反刍动物营养。E-mail: 1587870877@qq.com
基金资助:
国家重点研发计划(2017YFD0502005); 四川省肉牛创新团队(035Z2040)

Differences in jejunum microbes of goats with different phosphorus true digestibility

JIN Lei, WANG Lizhi^*, WANG Zhisheng, XUE Bai, PENG Quanhui

Animal Nutrition Institute, Sichuan Agricultural University, Chengdou 611130, China

Received:2018-11-05 Online:2019-07-25 Published:2019-08-07

摘要/Abstract

摘要： 动物对饲料磷消化率的提高对于降低养殖成本和减少环境污染均具有重要意义。通过高通量测序技术比较饲料磷真消化率不同的山羊空肠微生物结构的差异。本研究选取24只10月龄,雌性,平均体质量(24.25±2.47)kg的努比亚黑山羊作为试验动物,通过代谢试验筛选出高(HP)、低(LP)饲料磷真消化率组,每组各6只。分别采集HP、LP组山羊空肠内容物,提取微生物总DNA,利用高通量测序平台对细菌16S rRNA基因的高可变区进行测序,使用QIIME等软件对测序数据进行生物信息学分析。研究表明,在门水平,厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)在两组山羊空肠微生物中均为优势菌门,其中HP组厚壁菌门(Firmicutes)的相对丰度极显著低于LP组(P<0.01),而拟杆菌门(Bacteroidetes)的相对丰度极显著高于LP组(P<0.01);在属水平上,HP组Prevotella、Fibrobacter、Ruminococcus_2和Clostridium_sensu_stricto_1的相对丰度显著高于LP组(P<0.05),而Desulfovibrio和Victivallis的相对丰度显著低于LP组(P<0.05)。饲料磷真消化率不同的山羊空肠菌群结构存在显著差异。

关键词: 山羊, 磷真消化率, 空肠, 微生物

Abstract: Improving animal digestion and utilization efficiency of phosphorus were of great significance for reducing animal breeding costs and environmental pollution. The objective of this study was to compare the structure of jejunum microorganism of goats with different true digestibility of feed phosphorus using Illumina MiSeq sequencing technology. Twenty-four Nubian black goats, aged 10 months on average and weighed (24.25±2.47)kg, were selected as the experimental animals. The contents of jejunum of each goat in the HP and LP groups were collected and total genomic DNA was extracted, and the high variable region of 16S rRNA gene of bacteria was sequenced by high throughput sequencing platform. Bioinformatics analysis of sequencing data was carried out using QIIME software. At the phylum level, Firmicutes and Bacteroidetes were the dominant bacteria of the jejunum microbiota in goats, and the relative abundance of Firmicutes in HP group was extremely significantly lower than that in the LP group (P<0.01), while the relative abundance of Bacteroidetes was extremely significantly higher than that in the LP group(P<0.01). At the genus level, the relative abundances of Prevotella, Rikenellaceae_RC9_gut_group, Fibrobacter, Ruminococcus_2 and Clostridium_sensu_stricto_1 in HP group were significantly higher than that in the LP group (P<0.05), and the relative abundances of Desulfovibrio and Victivallis were significantly lower than that in LP group(P<0.05). There were significant differences in structure of jejunum microorganisms among goats with different true digestibility of feed phosphorus.

Key words: goat, true digestibility of phosphorus, jejunum, microorganism

中图分类号:

S827.5

金磊, 王立志, 王之盛, 薛白, 彭全辉. 饲料磷真消化率不同的山羊空肠菌群结构差异研究[J]. 浙江农业学报, 2019, 31(7): 1057-1065.

JIN Lei, WANG Lizhi, WANG Zhisheng, XUE Bai, PENG Quanhui. Differences in jejunum microbes of goats with different phosphorus true digestibility[J]. , 2019, 31(7): 1057-1065.

参考文献

[1] SUTTLE N, SUTTLE N.Mineral nutrition of livestock[J]. Cabi Bookshop, 2009, 215(6):1-8.
[2] LEI X G, STAHL C H.Nutritional benefits of phytase and dietary determinants of its efficacy[J]. Journal of Applied Animal Research, 2000, 17(1): 97-112.
[3] NOVAK J M, WATTS D W, HUNT P G, et al.Phosphorus movement through a coastal plain soil after a decade of intensive swine manure application[J]. Journal of Environment Quality, 2000, 29(4): 1310-1315.
[4] TILMAN D.Forecasting agriculturally driven global environmental change[J]. Science, 2001, 292(5515): 281-284.
[5] KEBREAB E, HANSEN A V, STRATHE A B.Animal production for efficient phosphate utilization: from optimized feed to high efficiency livestock[J]. Current Opinion in Biotechnology, 2012, 23(6): 872-877.
[6] BREVES G, SCHRÖDER B. Comparative aspects of gastrointestinal phosphorus metabolism[J]. Nutrition Research Reviews, 1991, 4(1): 125-140.
[7] SHEN Y R, FAN M Z, AJAKAIYE A, et al.Use of the regression analysis technique to determine the true phosphorus digestibility and the endogenous phosphorus output associated with corn in growing pigs[J]. The Journal of Nutrition, 2002, 132(6): 1199-1206.
[8] DILGER R N, ADEOLA O.Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing chicks fed conventional and low-phytate soybean meals[J]. Poultry Science, 2006, 85(4): 661-668.
[9] SCHRÖDER B, BREVES G. Mechanisms and regulation of calcium absorption from the gastrointestinal tract in pigs and ruminants: comparative aspects with special emphasis on hypocalcemia in dairy cows[J]. Animal Health Research Reviews, 2006, 7(1/2): 31-41.
[10] LEYTEM A B, THACKER P A.Phosphorus utilization and characterization of excreta from swine fed diets containing a variety of cereal grains balanced for total phosphorus[J]. Journal of Animal Science, 2010, 88(5): 1860-1867.
[11] YANG S L, MA S C, CHEN J, et al.Bacterial diversity in the rumen of Gayals (Bos frontalis), Swamp buffaloes (Bubalus bubalis) and Holstein cow as revealed by cloned 16S rRNA gene sequences[J]. Molecular Biology Reports, 2010, 37(4): 2063-2073.
[12] JAMI E, MIZRAHI I.Similarity of the ruminal bacteria across individual lactating cows[J]. Anaerobe, 2012, 18(3): 338-343.
[13] MESCHY F.Recent progress in the assessment of mineral requirements of goats[J]. Livestock Production Science, 2000, 64(1): 9-14.
[14] BRAVO D, SAUVANT D, BOGAERT C, et al.III. Quantitative aspects of phosphorus excretion in ruminants[J]. Reproduction Nutrition Development, 2003, 43(3): 285-300.
[15] PETERSEN G I, STEIN H H.Novel procedure for estimating endogenous losses and measurement of apparent and true digestibility of phosphorus by growing pigs[J]. Journal of Animal Science, 2006, 84(8): 2126-2132.
[16] FAN M Z, ARCHBOLD T, SAUER W C, et al.Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs[J]. The Journal of Nutrition, 2001, 131(9): 2388-2396.
[17] AKINMUSIRE A S, ADEOLA O.True digestibility of phosphorus in canola and soybean meals for growing pigs: Influence of microbial phytase[J]. Journal of Animal Science, 2009, 87(3): 977-983.
[18] AMMERMAN C B, FORBES R M, GARRIGUS U S, et al.Ruminant utilization of inorganic phosphates[J]. Journal of Animal Science, 1957, 16(4): 796-810.
[19] GUO W, LI Y, WANG L Z, et al.Evaluation of composition and individual variability of rumen microbiota in yaks by 16S rRNA high-throughput sequencing technology[J]. Anaerobe, 2015, 34: 74-79.
[20] CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al.QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 2010, 7(5): 335-336.
[21] EDGAR R C, HAAS B J, CLEMENTE J C, et al.UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics, 2011, 27(16): 2194-2200.
[22] SCHLOSS P D, WESTCOTT S L, RYABIN T, et al.Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Applied and Environmental Microbiology, 2009, 75(23): 7537-7541.
[23] HUSE S M, WELCH D M, MORRISON H G, et al.Ironing out the wrinkles in the rare biosphere through improved OTU clustering[J]. Environmental Microbiology, 2010, 12(7): 1889-1898.
[24] EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics, 2010, 26(19): 2460-2461.
[25] COLE J R, WANG Q, CARDENAS E, et al.The Ribosomal Database Project: improved alignments and new tools for rRNA analysis[J]. Nucleic Acids Research, 2009, 37(S1): 141-145.
[26] QUAST C, PRUESSE E, YILMAZ P, et al.The SILVA ribosomal RNA gene database project: improved data processing and web-based tools[J]. Nucleic Acids Research, 2012, 41(D1): 590-596.
[27] LOZUPONE C, LLADSER M E, KNIGHTS D, et al.UniFrac: an effective distance metric for microbial community comparison[J]. The ISME Journal, 2011, 5(2): 169-172.
[28] LANGILLE M G I, ZANEVELD J, CAPORASO J G, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature Biotechnology, 2013, 31(9): 814-821.
[29] FU L L, JIANG B H, LIU J L, et al.Genome sequence analysis of a flocculant-producing bacterium, Paenibacillus shenyangensis[J]. Biotechnology Letters, 2016, 38(3): 447-453.
[30] LIU ZHENGQUN, ZHANG ZUXIANG, ZHANG ZUXIANG, et al.Effects of basal diet composition on true total tract digestibility of phosphorus in dicalcium phosphate and monocalcium phosphate for growing pigs[J]. Chinese Journal of Animal Nutrition, 2016, 28(8):2542-2550.
[31] ABBASI F, LIU J B, ZHANG H F, et al.Effects of dietary total phosphorus concentration and casein supplementation on the determination of true phosphorus digestibility for broiler chickens[J]. Italian Journal of Animal Science, 2018, 17(1): 135-144.
[32] LIU J, BOLLINGER W, LEDOUX R, et al.Effects of dietary calcium: phosphorus ratios on apparent absorption of calcium and phosphorus in the small intestine, cecum, and colon of pigs[J]. Journal of Animal Science, 2000, 78(1): 106-109.
[33] JOHNSTON S L, WILLIAMS S B, SOUTHERN L L, et al.Effect of phytase addition and dietary calcium and phosphorus levels on plasma metabolites and ileal and total-tract nutrient digestibility in pigs[J]. Journal of Animal Science, 2004, 82(3): 705-714.
[34] SELLE P H, COWIESON A J, RAVINDRAN V.Consequences of calcium interactions with phytate and phytase for poultry and pigs[J]. Livestock Science, 2009, 124(1/2/3): 126-141.
[35] MUTUCUMARANA R K, RAVINDRAN V, RAVINDRAN G, et al.Measurement of true ileal phosphorus digestibility in maize and soybean meal for broiler chickens: comparison of two methodologies[J]. Animal Feed Science and Technology, 2015, 206: 76-86
[36] EKELUND A, SPÖRNDLY R, VALK H, et al. Effects of varying monosodium phosphate intake on phosphorus excretion in dairy cows[J]. Livestock Production Science, 2005, 96(2/3): 301-306.
[37] DE OLIVEIRA M N V, JEWELL K A, FREITAS F S, et al. Characterizing the microbiota across the gastrointestinal tract of a Brazilian Nelore steer[J]. Veterinary Microbiology, 2013, 164(3/4): 307-314.
[38] MAO S Y, ZHANG M L, LIU J H, et al.Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: membership and potential function[J]. Scientific Reports, 2015, 5: 16116.
[39] CHEN Y H, PENNER G B, LI M J, et al.Changes in bacterial diversity associated with epithelial tissue in the beef cow rumen during the transition to a high-grain diet[J]. Applied and Environmental Microbiology, 2011, 77(16): 5770-5781.
[40] PURUSHE J, FOUTS D E, MORRISON M, et al.Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche[J]. Microbial Ecology, 2010, 60(4): 721-729.
[41] YANKE L J, BAE H D, SELINGER L B, et al.Phytase activity of anaerobic ruminal bacteria[J]. Microbiology, 1998, 144(6): 1565-1573.
[42] WOOD T M, WILSON C A, STEWART C S.Preparation of the cellulase from the cellulolytic anaerobic rumen bacterium Ruminococcus albus and its release from the bacterial cell wall[J]. Biochemical Journal, 1982, 205(1): 129-137.
[43] DOERNER K C, WHITE B A.Assessment of the endo-1, 4-beta-glucanase components of Ruminococcus flavefaciens FD-1[J]. Applied and Environmental Microbiology, 1990, 56(6): 1844-1850.
[44] STEVENSON D M, WEIMER P J.Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR[J]. Applied Microbiology and Biotechnology, 2007, 75(1): 165-174.
[45] SIMPSON H L, CAMPBELL B J.Review article: dietary fibre-microbiota interactions[J]. Alimentary Pharmacology & Therapeutics, 2015, 42(2): 158-179.
[46] LU K, ABO R P, SCHLIEPER K A, et al.Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis[J]. Environmental Health Perspectives, 2014, 122(3): 284-291.
[47] VANESSA K. RIDAURA, JEREMIAH J. FAITH, FEDERICO E.REY, et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice[J]. Science, 2013, 341(6150):1079.

饲料磷真消化率不同的山羊空肠菌群结构差异研究

Differences in jejunum microbes of goats with different phosphorus true digestibility

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐民民, 黄莹, 李波, 徐艳, 张帅, 姚岭芸, 王政. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.
[2]	孙筱君, 沈琦, 吴逸飞, 姚晓红, 李园成, 孙宏, 王新, 汤江武, 葛向阳. 氨氮降解微生物的筛选和初步应用[J]. 浙江农业学报, 2020, 32(9): 1683-1691.
[3]	刘均, 沈佳敏, 沈建良, 刘雅丽. 不同包装方式对货架期冷鲜鸡微生物菌相变化的影响[J]. 浙江农业学报, 2020, 32(7): 1274-1280.
[4]	林辉, 张锦, 原倩宇, 叶静, 孙万春, 虞轶俊, 俞巧钢, 马军伟. 棘孢木霉和超微粉腐殖质改善连作土壤微生态[J]. 浙江农业学报, 2020, 32(6): 1060-1069.
[5]	史传奇, 胡宝忠, 于少鹏, 孟博, 杨春雪, 刘嘉, 丁俊男. 不同处理条件下金鱼藻净水效果与微生物群落变化[J]. 浙江农业学报, 2020, 32(6): 1070-1081.
[6]	刘涛, 张翅鹏, 郝瑶玲, 邱丽娟, 黄臣臣. 硫酸盐对土壤铁矿物还原转化及砷释放的影响[J]. 浙江农业学报, 2020, 32(4): 678-684.
[7]	王保君, 程旺大, 陈贵, 沈亚强, 沈盟, 袁晔, 王蕾, 张红梅. 氮肥调控对浙北地区秸秆全量还田稻田土壤及水稻产量的影响[J]. 浙江农业学报, 2020, 32(2): 183-190.
[8]	李美霖, 陈宇眺, 洪晓富, 乔宇颖, 王青霞, 陈喜靖, 沈阿林, 喻曼. 不同氮肥管理方式对稻田土壤微生物群落结构的影响[J]. 浙江农业学报, 2020, 32(2): 308-316.
[9]	李如意, 尹军峰, 邹纯. 红茶菌的国内外研究现状[J]. 浙江农业学报, 2020, 32(12): 2291-2302.
[10]	曾学琴, 柳陈坚, 杨雪, 李晓然. 高通量测序法检测奶牛乳房炎关联微生物群落结构及多样性[J]. 浙江农业学报, 2019, 31(9): 1437-1445.
[11]	董宇飞, 吕相漳, 张自坤, 贺洪军, 喻景权, 周艳虹. 不同栽培模式对辣椒根际连作土壤微生物区系和酶活性的影响[J]. 浙江农业学报, 2019, 31(9): 1485-1492.
[12]	王燕云, 赵龙杰, 郝春莉, 蔡尽忠. 生物有机肥对不同连作年限设施黄瓜土壤微生物数量和酶活性的影响[J]. 浙江农业学报, 2019, 31(4): 631-638.
[13]	张博伟, 王海静, 宋茂勇, 谢慧君, 张建. 四溴双酚A对土壤无机氮转化的影响及其微生物学机理初探[J]. 浙江农业学报, 2019, 31(4): 639-645.
[14]	王青霞, 陈喜靖, 喻曼, 沈阿林. 秸秆还田对稻田氮循环微生物及功能基因影响研究进展[J]. 浙江农业学报, 2019, 31(2): 333-342.
[15]	葛艺, 徐绍辉, 徐艳. 根际微生物组构建的影响因素研究进展[J]. 浙江农业学报, 2019, 31(12): 2120-2130.