外源草酸缓解马尾松根系铝毒

doi:10.3969/j.issn.1004-1524.2019.07.08

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (7): 1086-1095.DOI: 10.3969/j.issn.1004-1524.2019.07.08

外源草酸缓解马尾松根系铝毒

郭妮, 刘亚敏^*, 周文颖, 刘玉民, 张盛楠

西南大学资源环境学院,三峡库区生态环境教育部重点实验室,重庆 400715

收稿日期:2019-03-18 出版日期:2019-07-25 发布日期:2019-08-07
通讯作者: *刘亚敏,E-mail: yaminliu0511@163.com
作者简介:郭妮(1994—),女,陕西宝鸡人,硕士研究生,主要从事经济林栽培研究。E-mail:18883774200@163.com
基金资助:
中央林业改革发展资金科技推广示范项目(渝林科推〔2017〕07号)

Alleviation of aluminum stress by exogenous oxalic acid in root system of Pinus massoniana Lamb.

GUO Ni, LIU Yamin^*, ZHOU Wenying, LIU Yumin, ZHANG Shengnan

Key Laboratory of Eco-Environments in Three Gorges Region, Ministry of Education, College of Resources and Environment, Southwest University, Chongqing 400715,China

Received:2019-03-18 Online:2019-07-25 Published:2019-08-07

摘要/Abstract

摘要： 以耐铝型(FJ5)和铝敏感型(GD20)马尾松幼苗为材料,采用水培法研究外源添加草酸对铝胁迫下马尾松幼苗根系形态及生理特性的影响,并探讨控制草酸缓解马尾松根系铝毒害的主导生理因子,旨在为土壤酸化地区调控马尾松铝毒害提供理论依据。结果表明,铝胁迫下马尾松幼苗总根长、根表面积、根系体积、根系活力等均有所降低,根系抗氧化酶(超氧化物歧化酶、过氧化物酶和过氧化氢酶)活性、渗透调节物质(可溶性糖、可溶性蛋白和脯氨酸)含量均显著(P<0.05)升高,膜质过氧化程度加剧,且GD20比FJ5的变化更强烈,说明活性铝可对马尾松幼苗产生一定的毒害,且对铝敏感型的毒害作用更大。外源添加草酸后,马尾松根系活力升高,根系中丙二醛和渗透调节物质含量降低,抗氧化酶活性维持较高水平,当草酸浓度≤0.2 mmol·L^-1时,根系铝和根尖铝的含量减低,且GD20的变化幅度大于FJ5,说明外源草酸能有效缓解马尾松幼苗受到的铝毒害作用,且对铝敏感型的缓解效果好于耐铝型。外源添加不同浓度的草酸对马尾松根系生理指标的作用效果不同,以外源添加草酸浓度为0.2 mmol·L^-1时缓解铝毒害效果最好。主成分分析结果显示,脯氨酸和可溶性蛋白含量可能是控制草酸缓解马尾松铝毒害的主导生理因子。

关键词: 马尾松, 铝胁迫, 草酸

Abstract: Two different families of Pinus massoniana Lamb., namely aluminum-resistant type FJ5 and aluminum-sensitive type GD20 were used as test materials to provide theoretical basis for the regulation of aluminum toxicity in P. massoniana Lamb. in soil acidification areas. The effects of oxalic acid on root morphology and physiological characteristics of P. massoniana Lamb. seedlings under aluminum stress were studied, and the dominant physiological factors affecting alleviation effect of oxalic acid were explored. It was shown that under aluminum stress, the total root length, root surface area, root volume and root activity of P. massoniana Lamb. seedlings were reduced, while the activities of root antioxidant enzymes (superoxide dismutase, peroxidase, catalase), osmotic adjustment substances (soluble sugar, soluble protein and proline) significantly (P<0.05) increased, and membrane lipid peroxidation aggravated. The variation of these indices in GD20 was higher than that in FJ5. It indicated that active aluminum could produce toxicity to P. massoniana Lamb., and active aluminum had greater toxic effects on aluminum sensitive species. After application of exogenous oxalic acid, the root activity of P. massoniana Lamb. increased, the content of malondialdehyde and osmotic adjustment substances in roots decreased, and the antioxidant enzymes activities maintained a high level. When oxalic acid concentration did not exceed 0.2 mmol·L^-1, the aluminum content in root and root tip decreased. The variation of these indices with oxalic acid application in GD20 was greater than that in FJ5. These results indicated that exogenous oxalic acid could effectively alleviate the aluminum toxicity of P. massoniana Lamb. seedlings, and the alleviation effect on aluminum-sensitive type was better than that on aluminum-resistant type. The alleviation effect on P. massoniana Lamb. varied with different concentrations of exogenous oxalic acid, and the optimum application concentration was 0.2 mmol·L^-1. It was revealed by principal component analysis that contents of proline and soluble protein might be the dominant physiological factors to regulate alleviation effect of oxalic acid on aluminum toxicity.

Key words: Pinus massoniana Lamb., aluminum stress, oxalic acid

中图分类号:

郭妮, 刘亚敏, 周文颖, 刘玉民, 张盛楠. 外源草酸缓解马尾松根系铝毒[J]. 浙江农业学报, 2019, 31(7): 1086-1095.

GUO Ni, LIU Yamin, ZHOU Wenying, LIU Yumin, ZHANG Shengnan. Alleviation of aluminum stress by exogenous oxalic acid in root system of Pinus massoniana Lamb.[J]. , 2019, 31(7): 1086-1095.

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外源草酸缓解马尾松根系铝毒

Alleviation of aluminum stress by exogenous oxalic acid in root system of Pinus massoniana Lamb.

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