马铃薯致病疫霉及其拮抗菌的筛选与鉴定

doi:10.3969/j.issn.1004-1524.2020.05.12

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 840-848.DOI: 10.3969/j.issn.1004-1524.2020.05.12

马铃薯致病疫霉及其拮抗菌的筛选与鉴定

刘紫英¹, 袁斌², 肖花美², 吴永飞¹, 刘小林^2,*, 胡祥飞¹

1.宜春学院化学与生物工程学院,江西宜春 336000;
2.宜春学院生命科学与资源环境学院,江西宜春 336000

收稿日期:2020-01-16 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: *,刘小林,E-mail: 13507058860@163.com
作者简介:刘紫英(1979—),女,江西万年人,硕士,副教授,研究方向为植物病理学。E-mail: yingziliu2008@163.com
基金资助:
宜春市2017年度创新驱动“5511”工程重点研发计划(20604021)

Screening, identification of Phytophthora infestans and its antagonistic bacterial strain

LIU Ziying¹, YUAN Bin², XIAO Huamei², WU Yongfei¹, LIU Xiaolin^2,*, HU Xiangfei¹

1. School of Chemical and Biological Engineering, Yichun University, Yichun 336000, China;
2. College of Life Science and Resources Environment, Yichun University, Yichun 336000, China

Received:2020-01-16 Online:2020-05-25 Published:2020-05-29

摘要/Abstract

摘要： 采用组织分离法分离鉴定马铃薯致病疫霉,同时比较3株拮抗菌对马铃薯致病疫霉的抗菌效果,以期获得1株更具有生防潜力的拮抗细菌,并加以鉴定。用涂布分离法得到3株拮抗菌,通过形态学和分子生物学鉴定马铃薯致病疫霉和这3株拮抗菌。同时比较这些拮抗菌对马铃薯致病疫霉游动孢子释放、休止孢萌发和孢子囊直接萌发的抑制效果,分析菌液对马铃薯晚疫病的预防效果。结果鉴定出1株马铃薯致病疫霉PLB-2 Phytophthora infestans和1株具有明显抑制效果的拮抗细菌WZ-502 Brachybacterium sp.。菌株WZ-502的菌液对马铃薯致病疫霉游动孢子释放、孢子囊直接萌发和休止孢萌发有较好的抑制效果。菌株WZ-502菌液浓度为3.65×10⁷ CFU·mL^-1、稀释度为10^-2~10^-3时,对马铃薯致病疫霉的抑制效果均明显,稀释度为10^-3时,马铃薯致病疫霉孢子囊萌发率和释放率均在40%以下。因此,菌株WZ-502对马铃薯致病疫霉抗菌效果显著,具有一定的生防潜力。

关键词: 马铃薯, 致病疫霉, 拮抗细菌, 游动孢子释放, 孢子囊萌发

Abstract: Phytophthora infestans was identified from potato via tissue isolation method and the antibacterial effects of three antagonistic bacterium to it were compared in order to get the potential one. They were isolated by coating method and identified by morphology and molecular biology method. The inhibitory effects of Phytophthora infestans were studied including the release of zoospore, germination of resting spore and germination of sporangium. The preventive effect of the bacterial solution was also analyzed. The results showed that a PLB-2 Phytophthora infestans and a WZ-502 Brachybacterium sp. of potato were separated and identified. The strain WZ-502 had significant inhibitory effect on the release of zoospores, direct germination of sporangium and germination of resting spores. The strain WZ-502 (concentration was 3.65×10⁷ CFU·mL^-1) at dilutions grade of 10^-2-10^-3 was effective, and the rates of sporangium germination and the release of Phytophthora infestans were both below 40% when the dilution was 10^-3. The strain WZ-502 had a good antibacterial effect on Phytophthora infestans.

Key words: potato, Phytophthora infestans, antagonistic bacteria, zoospores release, sporangium germination

中图分类号:

S435.3

刘紫英, 袁斌, 肖花美, 吴永飞, 刘小林, 胡祥飞. 马铃薯致病疫霉及其拮抗菌的筛选与鉴定[J]. 浙江农业学报, 2020, 32(5): 840-848.

LIU Ziying, YUAN Bin, XIAO Huamei, WU Yongfei, LIU Xiaolin, HU Xiangfei. Screening, identification of Phytophthora infestans and its antagonistic bacterial strain[J]. , 2020, 32(5): 840-848.

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马铃薯致病疫霉及其拮抗菌的筛选与鉴定

Screening, identification of Phytophthora infestans and its antagonistic bacterial strain

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