施氮量对马铃薯氮素积累及功能叶片生理特性的影响

doi:10.3969/j.issn.1004-1524.2016.09.02

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (9): 1454-1461.DOI: 10.3969/j.issn.1004-1524.2016.09.02

施氮量对马铃薯氮素积累及功能叶片生理特性的影响

何彩莲¹, 郑顺林^{1, *}, 王沛裴¹, 赵婷婷¹, 袁继超¹, 何卫²

1.四川农业大学农学院,四川成都 611130;
2.西南地区作物栽培重点实验室,四川成都 611130

收稿日期:2015-12-30 出版日期:2016-09-15 发布日期:2016-11-23
通讯作者: 郑顺林,E-mail: zhengshunlin123@163.com
作者简介:何彩莲(1991—),女,四川达州人,硕士研究生,研究方向为马铃薯高产栽培技术。E-mail: hecailian818041@163.com
基金资助:
国家科技支撑计划(2012BAD06B0407); 四川省育种攻关配套项目(2011NZ0098-15-5)

Effect of nitrogen application rate on nitrogen accumulation and physiological characteristics of functional leaf in potato

HE Cai-lian¹, ZHENG Shun-lin^{1, *}, WANG Pei-pei¹, ZHAO Ting-ting¹, YUAN Ji-chao¹, HE Wei²

1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Southwest Crop Cultivation, Chengdu 611130, China

Received:2015-12-30 Online:2016-09-15 Published:2016-11-23

摘要/Abstract

摘要： 为探讨不同季节马铃薯氮素高效利用的相关机理,指导不同季节马铃薯的氮肥运筹,提高氮肥施用效率。通过春、秋季盆栽试验,比较6个施氮水平下春、秋马铃薯氮素积累及功能叶片生理特性的差异。结果表明:(1)随施氮量的增加,春马铃薯硝态氮含量增加,秋马铃薯则先增后减;春、秋马铃薯硝酸还原酶活性均随施氮量增加呈现先增后减。同等肥力下,块茎形成期与块茎成熟期春马铃薯的硝酸还原酶活性峰值比秋马铃薯分别高出35.3%、74.0%。(2)春、秋马铃薯植株氮含量、氮素积累量随施氮量的增加而增加;同一生育时期同等肥力下,春马铃薯植株氮含量、氮素积累量均高于秋马铃薯。(3)生理指标中叶片硝态氮与植株氮含量相关系数较高。

关键词: 马铃薯, 施氮水平, 硝态氮, 硝酸还原酶, 氮素积累

Abstract: Study on the mechanism of high efficiently utilizing nitrogen of potatoes in different seasons could direct the application of nitrogen fertilizer and promote the utilization efficiency of nitrogen fertilizer. Through pot culture experiment in spring and autumn, the differences of nitrogen accumulation and physiological property of function potato leaves in six nitrogen levels were studied. The results could be summarized as follows: (1) With the increasing rate of nitrogen applied, the nitrate nitrogen content of spring potatoes increased, but that of autumn potatoes decreased after first increase. And the nitrate reductase (NR) activity of potatoes both in spring and autumn increased first and then decreased. Under the same nitrogen level, the peak value of NR activity of spring potatoes in tuber formation and maturity stages were 35.3% and 74.0% higher than those in autumn. (2) The nitrogen content and nitrogen accumulation of potato plants both in spring and autumn increased with the inceasing nitrogen fertilizer. But these two indicators of potatoes in spring were both higher than those in autumn under the same growth period and nitrogen levels. (3) There was a higher correlation coefficient between the nitrate nitrogen content and the total nitrogen content in potato plants, which could induct the effective utilization of nitrogen.

Key words: potatoe, nitrogen fertilizer level, nitrate nitrogen, nitrate reductase, nitrogen accumulation

中图分类号:

S532

何彩莲, 郑顺林, 王沛裴, 赵婷婷, 袁继超, 何卫. 施氮量对马铃薯氮素积累及功能叶片生理特性的影响[J]. 浙江农业学报, 2016, 28(9): 1454-1461.

HE Cai-lian, ZHENG Shun-lin, WANG Pei-pei, ZHAO Ting-ting, YUAN Ji-chao, HE Wei. Effect of nitrogen application rate on nitrogen accumulation and physiological characteristics of functional leaf in potato[J]. , 2016, 28(9): 1454-1461.

参考文献

[1] 孙传范, 曹卫星, 戴廷波. 土壤-作物系统中氮肥利用率的研究进展[J]. 土壤, 2001 (2): 64-69.
SHUN C F, CHAO W X, DAI T B. Advances in the study of nitrogen use efficiency in soil crop system [J]. Soil , 2001 (2): 64-69. (in Chinese)
[2] 葛晓光, 王晓雪, 刘秀茹, 等. 试验研究长期定位施氮条件下蔬菜对氮素的吸收及利用的研究[J]. 中国蔬菜, 1998 (5): 1-5.
GE X G, WANG X X, LIU X R, et al. Studies on absorption and utilization of nitrogen under experiment of long-term and fixed position nitrogen application in vegetable field [J]. China Vegetables , 1998 (5): 1-5. (in Chinese with English abstract)
[3] 田丰, 张永成. 马铃薯光合速率与产量相关性研究[J]. 种子, 2004, 23(6): 30-31.
FENG T, ZHANG Y C. Correlation of photosynthetic rate and yield potato [J]. Seed , 2004, 23(6): 30-31. (in Chinese with English abstract)
[4] 杨宪龙, 路永莉, 同延安, 等. 长期施氮和秸秆还田对小麦-玉米轮作体系土壤氮素平衡的影响[J]. 植物营养与肥料学报, 2013, 19(1): 68-77.
YANG X L, LU Y L, TONG Y A, et al. Effects of long-term N application and straw returning on N budget under wheat-maize rotation system [J]. Plant Nutrition and Fertilizer Science , 2013, 19(1): 68-77. (in Chinese with English abstract)
[5] FAGERIA N K, BALIGAR V C. Enhancing nitrogen use efficiency in crop plants [J]. Advances in Agronomy , 2005, 88(5): 97-185.
[6] 蔡红光, 米国华, 陈范骏, 等. 玉米叶片SPAD值、全氮及硝态氮含量的品种间变异[J]. 植物营养与肥料学报, 2010, 16(4): 866-873.
CAI H G, MI G H, CHEN F J, et al. Genotypic variation of leaf SPAD value, nitrogen and nitrate content in maize [J]. Plant Nutrition and Fertilizer Science , 2010, 16(4): 866-873. (in Chinese with English abstract)
[7] 贾良良, 陈新平, 张福锁. 叶绿素仪与植株硝酸盐浓度测试对冬小麦氮营养诊断准确性的比较研究[J]. 华北农学报, 2007, 22(6): 157-160.
JIA L L. CHEN X P, ZHENG F S. The comparision of spad chlorophyll meter and sap nitrate test as N diagnosis methods for winter wheat [J]. Acta Agriculture Boreali-Sinica , 2007, 22(6): 157-160. (in Chinese with English abstract)
[8] 李占成, 李玮, 梁秀枝, 等. 叶绿素仪在玉米氮营养诊断及推荐施肥中的研究与应用[J]. 作物杂志, 2011 (4): 58-62.
LI Z C. LI W. LIANG X Z, et al. Application of chlorophyll meter in nitrogen diagnosis and fertilization in spring corn in cold zones [J]. Crops , 2011 (4): 58-62. (in Chinese with English abstract)
[9] 李志宏, 张云贵, 刘宏斌, 等. 叶绿素仪在夏玉米氮营养诊断中的应用[J]. 植物营养与肥料学报, 2005, 11(6): 764-768.
LI Z H, ZHENG Y G, LIU H B, et al. Application of chlorophyll meter on N nutritional diagnosis for summer corn [J]. Plant Nutrition and Fertilizer Science , 2005, 11(6): 764-768. (in Chinese with English abstract)
[10] 郭战玲, 沈阿林, 寇长林, 等. 不同小麦品种开花后硝酸还原酶活性与氮效率的关系[J]. 中国农学通报, 2008, 24(5): 219-223.
GU Z L, SHEN A L, KOU C Z. et al. The relationship between NRA and nitrogen efficiency of different wheat varieties after flowering [J]. Chinese Agricultural Science Bulletin , 2008, 24(5): 219-223. (in Chinese with English abstract)
[11] 赵洪祥. 吉林省不同年代大豆品种硝酸还原酶活性变化研究[D]. 长春: 吉林农业大学, 2007.
ZHAO H X. Studies on changes of nitrate reductase activity of soybean cultivars with the year of release in Jilin Province [D]. Changchun: Jilin Agricultural University, 2007. (in Chinese with English abstract)
[12] 隽英华, 孙文涛, 韩晓日, 等. 春玉米功能叶片生理特征及产量对施氮的响应[J]. 核农学报, 2015, 29(2): 391-396.
JUAN Y H, SHUN W T, HAN X R, et al. Response of spring maize to nitrogen application in physiological characteristics of functional leaves and grain yield [J]. Journal of Nuclear Agricultural Sciences , 2015, 29(2): 391-396. (in Chinese with English abstract)
[13] 吕丽华, 陶洪斌, 王璞, 等. 施氮量对夏玉米碳、氮代谢和氮利用效率的影响[J]. 植物营养与肥料学报, 2008, 14(4): 630-637.
LYU L H, TAO H B, WANG P, et al. The effect of nitrogen application rate on carbon and nitrogen metabolism and nitrogen use efficiency of summer maize [J]. Plant Nutrition and Fertilizer Science , 2008, 14(4): 630-637. (in Chinese with English abstract)
[14] 李潮海, 刘奎, 连艳鲜. 玉米碳氮代谢研究进展[J]. 河南农业大学学报, 2000, 34(4): 318-323.
LI C H, LIU K, LIAN Y X. The recent progress of research on carbon and nitrogen metabolism in maize [J]. Journal of Henan Agricultural University , 2000, 34(4): 318-323. (in Chinese with English abstract)
[15] 李鹏程, 董合林, 刘爱忠, 等. 施氮量对棉花功能叶片生理特性、氮素利用效率及产量的影响[J]. 植物营养与肥料学报, 2015, 21(1): 81-91.
LI P C, DONG H L, LIU A Z, et al. Effects of nitrogen application rates on physiological characteristics of functional leaves, nitrogen use efficiency and yield of cotton [J]. Plant Nutrition and Fertilizer Science , 2015, 21(1): 81-91. (in Chinese with English abstract)
[16] 王晓巍, 马欣, 周连仁, 等. 施氮对玉米产量和氮素积累及相关生理指标的影响[J]. 玉米科学, 2012, 20(5): 121-125.
WANG X W, MA X, ZHOU L R, et al. Effect of nitrogen fertilization on corn yield, nitrogen accumulation and physiology index [J]. Journal of Maize Sciences , 2012, 20(5): 121-125. (in Chinese with English abstract)
[17] 陈贵, 张红梅, 沈亚强, 等. 嘉兴地区不同基因型水稻的氮利用效率研究[J]. 浙江农业学报, 2015, 27(11): 1965-1970.
CHEN G, ZHANG H M, SHEN Y Q, et al. Characteristics of N utilization efficiency in different rice genotypes in Jiaxing area [J]. Acta Agriculturae Zhejiangensis , 2015, 27(11): 1965-1970. (in Chinese with English abstract)
[18] 郑许松, 成丽萍, 王会福, 等. 施肥调节对稻纵卷叶螟发生和水稻产量的影响[J]. 浙江农业学报, 2015, 27(9): 1619-1624.
ZHENG X S, CHENG L P, WANG H F, et al. Effects of fertilizer regulation on occurrence of leaffolder, Cnaphalocrocts medinalis and rice production [J]. Acta Agriculturae Zhejiangensis , 2015, 27(9): 1619-1624. (in Chinese with English abstract)
[19] 熊庆娥. 植物生理学实验教程[M]. 成都: 四川科学技术出版社, 2010: 42-44.
[20] 赵亚华, 高向阳. 生物化学实验技术教程[M]. 广州: 华南理工大学出版社, 2000: 146.
[21] 李合生, 孙群, 赵世杰. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 184, 191.
[22] 童庆禧, 张兵, 郑兰芬. 高光谱遥感的多学科应用[M]. 北京: 电子工业出版社, 2006: 20-80.
[23] 方慧, 宋海燕, 曹芳, 等. 油菜叶片的光谱特征与叶绿素含量之间的关系研究[J]. 光谱学与光谱分析, 2007, 27(9): 1731-1734.
FANG H, SONG H Y, CAO F, et al. Study on the relationship between spectral properties of oilseed rape leaves and their chlorophyll content [J]. Spectroscopy and Spectral Analysis , 2007, 27(9): 1731-1734. (in Chinese with English abstract)
[24] 张金恒, 王珂, 王人潮. 叶绿素计SPAD-502在水稻氮素营养诊断中的应用[J]. 西北农林科技大学学报(自然科学版), 2003, 31(2): 177-180.
WANG J H, WANG K, WANG R C. Application o f chlorophyll meter SPAD-502 in diagnosis of nitrogen status and nitrogenous fertilizer in rice [J]. Journal of Northwest A & F University ( Natural Science Edition ), 2003, 31(2): 177-180. (in Chinese with English abstract)
[25] 卿国林. 稻草覆盖对稻茬免耕秋玉米生理特征及产量的影响[J]. 贵州农业科学, 2009, 37(11): 38-40.
QING G L. Effects of rice straw mulching on physiological characteristics and yield of autumn maize with no-tillage and rice stubbles [J]. Guizhou Agricultural Sciences , 2009, 37(11): 38-40. (in Chinese with English abstract)
[26] 申丽霞, 王璞. 玉米穗位叶碳氮代谢的关键指标测定[J]. 中国农学通报, 2009, 25(24): 155-157.
SHEN L X, WANG P. Determination of C-N metabolism indices in ear-leaf of maize ( Zea mays L) [J]. Chinese Agricultural Science Bulletin , 2009, 25(24): 155-157. (in Chinese with English abstract)
[27] 李东方, 李世清, 张胜利, 等. 冬小麦叶片硝酸还原酶活性对氮反应的敏感性研究[J]. 安徽农业科学, 2006, 34(17): 27-41.
LI D F, LI S Q, ZHANG S L, et al. Responsible sensitivity of nitrate reductase activity of winter wheat to n-fertilizer [J]. Journal of Anhui Agricultural Sciences , 2006, 34(17): 27-41. (in Chinese with English abstract)
[28] 李利. 不同施氮量对马铃薯氮素吸收、积累及利用的影响[J]. 山西农业科学, 2012, 40(12): 1292-1295.
LI L. Effect of nitrogen application rates on nitrogen absorption, accumulation and utilization [J]. Journal of Shanxi Agricultural Sciences , 2012, 40(12): 1292-1295. (in Chinese with English abstract)
[29] 雷武生, 王永平. 不同氮肥运筹对丘陵地区春播马铃薯产量及氮素吸收利用的影响[J]. 广东农业科学, 2013 (21): 61-65.
LEI W S, WANG Y P. Effect of different nitrogen management strategies on nitrogen uptake, utilization and yield of spring potato in hilly regions [J]. Guangdong Agricultural Sciences , 2013(21): 61-65. (in Chinese with English abstract)
[30] 李井会. 不同氮肥运筹下马铃薯氮素利用特性及营养诊断的研究[D]. 长春: 吉林农业大学, 2006.
LI J H. Characteristics of nitrogen utilization under different nitrogen strategies and nitrogen diagnosis in potato [D]. Changchun: Jilin Agricultural University, 2006. (in Chinese with English abstract)

编辑推荐

Metrics

阅读次数

全文

1484

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	1	0	0	1483

来源	本网站	其他网站

次数	993	491
比例	67%	33%

摘要

553

最新录用	在线预览	正式出版

0	0	553

来源	本网站	其他网站

次数	549	4
比例	99%	1%

施氮量对马铃薯氮素积累及功能叶片生理特性的影响

Effect of nitrogen application rate on nitrogen accumulation and physiological characteristics of functional leaf in potato

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	梁丽琴, 杨瑞, 郜刚. 马铃薯StUOXs基因家族的生物信息学分析[J]. 浙江农业学报, 2020, 32(9): 1523-1532.
[2]	许建民, 史和娣, 史培华, 张泽洋, 徐志刚. 不同光质条件下马铃薯光响应曲线拟合模型的比较[J]. 浙江农业学报, 2020, 32(5): 753-761.
[3]	刘紫英, 袁斌, 肖花美, 吴永飞, 刘小林, 胡祥飞. 马铃薯致病疫霉及其拮抗菌的筛选与鉴定[J]. 浙江农业学报, 2020, 32(5): 840-848.
[4]	韩悌倩, 刘震, 刘玉汇, 张小静, 王丽, 张俊莲. 减氮及有机替代对马铃薯根系形态和产量的影响[J]. 浙江农业学报, 2020, 32(12): 2111-2118.
[5]	马杰, 郑好, 周平, 陈春艳, 吴瑞, 马维, 宋雷, 孙勃. 马铃薯油菜素内酯信号激酶基因StBSKs的克隆与序列分析[J]. 浙江农业学报, 2019, 31(8): 1224-1230.
[6]	杨亚亚, 吴娜, 刘吉利, 杨娜娜, 蔡明, 何海锋. 马铃薯-燕麦间作对马铃薯氮含量和土壤氮素的影响[J]. 浙江农业学报, 2019, 31(12): 1955-1962.
[7]	程亮. 青海省部分地区马铃薯Y病毒cp基因序列分析[J]. 浙江农业学报, 2019, 31(11): 1888-1895.
[8]	洪森荣, 张铭心, 叶思雨, 宁本松. 高山马铃薯种质资源遗传多样性的同工酶分析[J]. 浙江农业学报, 2018, 30(9): 1445-1453.
[9]	王信, 程亮, 王亚艺, 高旭升, 李松龄. 青海省马铃薯黑胫病病原菌的鉴定[J]. 浙江农业学报, 2018, 30(8): 1369-1375.
[10]	唐晓, 邓孟胜, 邹雪, 李立芹, 祝渊智, 王西瑶. 马铃薯StDWF1基因克隆及表达分析[J]. 浙江农业学报, 2018, 30(6): 909-917.
[11]	江林娟, 邹雪, 黄雪丽, 倪苏, 李立芹, 杨世民. 响应面法优化马铃薯茎段高效再生体系[J]. 浙江农业学报, 2018, 30(6): 918-925.
[12]	宋西娇, 谢礼, 宣裕吉, 王芳, 严成其, 陈剑平. 复合侵染的马铃薯花叶病病原诊断[J]. 浙江农业学报, 2018, 30(1): 99-105.
[13]	潘晓艺, 蔺凌云, 尹文林, 徐洋, 姚嘉赟, 袁雪梅, 郑群艳, 沈锦玉. 氨氧化菌的分离鉴定及其在罗氏沼虾育苗废水中的应用[J]. 浙江农业学报, 2017, 29(6): 894-902.
[14]	尹明华, 刘燕, 郁雪婷, 李远芳, 周榕, 范亚红, 罗玉婕, 万小霞. 怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析[J]. 浙江农业学报, 2017, 29(10): 1699-1705.
[15]	沈升法，吴列洪，李兵. 春马铃薯早收栽培的块茎生育规律[J]. 浙江农业学报, 2016, 28(3): 389-.